The invention relates to a spectinomycin colloidal gold detection card. According to the method, a sample liquid absorbing part, a colloidal gold labeling part, a detection reaction part and a water absorbing part are orderly stuck on a backing of test paper. The detection reaction part is coated by an antigen 1 bar which is applied to detection and is used as a detection line, and at the same time is coated by an IgG1 bar which resists the second species of animal protein and is used as a reference line. The rapid detection test paper bar has high specificity, can carry out semi quantitative detection, can be used at the environmental temperature of 4 to 40 DEG C, and is suitable for an individual farmer, a food hygiene and quality inspection department, customs and other animal source food to carry out rapid detection on spectinomycin residues. The spectinomycin colloidal gold detection card provided by the invention has the advantages of high specificity, high sensitivity, simple and convenient operation and the like, and can carry out semi quantitative detection.