92 results about "Monensin" patented technology

The invention discloses a formula of a special completely mixed pellet feed for fattening sheep and a preparation method. The formula comprises corns, barley straws, sunflower cakes, alfalfa meal, cottonseed meal, wheat middling, malt roots, soybean meal, stone powders, molasses, puffing urea, baking soda, salt, 0.2% of polymineral for mutton sheep, calcium hydrogen phosphate, multivitamins for mutton sheep, monensin, a sweetener and a flavouring agent, wherein nutrients comprise dry matters, crude protein, calcium, total phosphorus, the salt, digestible energy for sheep and coarse fiber. The preparation method comprises the steps of: mixing the stone powder, the baking soda, the salt, the 0.2% of polymineral for mutton sheep, the calcium hydrogen phosphate, the multivitamins for mutton sheep, the monensin, the sweetener and the flavouring agent to prepare a semi-finished product A; grinding the corns, the sunflower cakes, the alfalfa meal, the puffing urea, the cottonseed meal, the wheat middling, the malt root, the soybean meal and the barley straws; and evenly mixing the materials by adopting a mixing machine, preparing the materials to be granular through a granulating process, and spraying the molasses on the surface of the pellet feed through a spraying process. The special completely mixed pellet feed for fattening sheep accords with the national green food concept, and has a good development prospect.

The invention discloses a fattening cattle concentrate supplement, which comprises the following components in parts by weight: 154-470 parts of maize, 50-100 parts of bran, 61-200 parts of cotton seed meal, 100-300 parts of palm meal, 50-220 parts of soybean meal, 30-200 parts of DDGS (Distillers Dried Grains with Soluble), 5-20 parts of bicalcium phosphate, 7-20 parts of rock flour, 2-10 parts of common salt, 8-15 parts of fattening cattle premix and 3-15 parts of rumen-protected lysine, wherein the fattening cattle premix is formed by mixing cattle composite vitamin, cattle composite microelement, yiduo enzyme (for cattle), monensin, bio-yeasture and an antioxidant. The fattening cattle concentrate supplement is full in nutrition, the utilization rate of the feed can be increased, the problem of overfeeding of beef cattle is solved, the feed waste is reduced, the feeding time of the cattle can be obviously shortened, the labor time of workers is shortened, the labor intensity is reduced, and the labor quota is improved, so that the production cost of the beef cattle is greatly reduced.

The invention discloses a straw type beef cattle complete pellet feed which is prepared from corn, bean pulp, cottonseed meal, corn distillers' grains, wheat bran, flour, brewer's grains, straws, apple pomace, sucrose, salt, calcium hydrophosphate, calcium powder, baking soda, magnesium oxide, a yeast culture, a sweetening agent, rumen-protected methionine, multivitamin, a microelement additive, a complex enzyme preparation and monensin. The straw type beef cattle complete pellet feed can feed beef cattle in a fattening period, has balanced dietary nutrition so as to completely meet the production demands of beef cattle, greatly increases the digestive utilization of fibrous raw materials such as straw, ensures the normal physiological function of rumen of the beef cattle, and meanwhile greatly reduces the feeding program of farmers and increases the production efficiency. Thus, the straw type beef cattle complete pellet feed has a large market demand and an economic benefit. Meanwhile, the straw is changed into wealth through reasonable nutritional match and a special preparation mode, so that the complete pellet feed has a relatively high social value.

The invention relates to a daily-ration beef and a preparation method thereof. The daily-ration beef is prepared by grinding and mixing maize, wheat, bean pulp, bran, DDGS (distillers dried grains with soluble), cottonseed meal, urea, ruminant microecologics, a mold removal agent, stone powder, calcium hydrogen phosphate, magnesium oxide, sodium bicarbonate, salt, monensin, a beef premix, all-cotton seeds, silages (air-dried matters), alfalfa and straws. The daily-ration beef combines concentrates with roughages, is reasonable in mixing, balanced in nutrition, high in digestion rate and good in palatability, and can be used for remarkably improving the health of beef rumens, increasing the beef growth speed, remarkably promoting the health and the daily gain of the beef rumens, shortening the output time and greatly increasing the economical benefit of beef fattening.

The invention relates to fodder and a use method, in particular to concentrated calf fodder and a use method thereof. The fodder comprises protein fodder and a premix compound, wherein the protein fodder is prepared from raw materials of bean cakes, corn protein powder, cotton dregs, complex enzymes, and the like; and the premix compound is prepared from common salt, fluorspar powder, magnesium sulfate, sodium diacetate, calcium hydrogen phosphate, soda, sodium humate, monensin, vitamins and trace elements of copper, iron, manganese, zinc, cobalt, and the like. The use method comprises the following steps: manually feeding calves at age of 3-5 days with a mixture of the concentrated calf fodder and a certain amount of milk; and stopping feeding the milk when the calves are at age of 60 days; and coming into a fatting period when the calves are at age of 120 days. The calf fodder has complete nutrients, reasonable proportion and low cost and is fed to the calves in a suckling period to substitute a great amount of milk, improve the commodity rate of the milk, facilitate the growth and the development of the calves, shorten the suckling period by 50 percent, increase the weight-gaining speed by about two times, shorten the deliver time by one half, increase the current fertility rate of milk cows by about two times, realize perennial milking, facilitate mechanical milking and scaled stabling management and remarkably improve the economic benefits, the social benefits and ecological benefits of cattle rearing in a plurality of aspects.

The invention relates to a beaver rabbit feed, which belongs to the feed field. The beaver rabbit feed mainly comprises the components of robenidine hydrochloride, monensin, salinomycin, bacitracin, garlicin, feed yeast, feed grade magnesium sulphate, feed grade DL-methionine, feed grade L-lysine hydrochloride, common salt, sulfur, calcium hydrophosphate, copper element, zinc element, iron element, choline chloride and vitamin complex premix, etc. The beaver rabbit feed can ensure that the conception rate of female beaver rabbits is improved to more than 93 percent and even to 100 percent; by adopting the beaver rabbit feed, the survival rate at 60 days of the beaver rabbits in the growing period can be improved to about 95 percent, and the growth cycle can be reduced by about half a month; simultaneously the growth of the hair of the beaver rabbit can be promoted, and the density, thickness uniformity and color brightness of the hair can be improved.

The invention relates to a meat lamb efficient fattening premix compound prescription, which belongs to the technical field of an animal husbandry feed additive. The invention aims to solve the problems that a meat lamb efficient fattening premix compound which is engaged with ration fine fodder to be used is provided, the adoptive scheme is as follows: the invention is prepared by the following solid raw materials according to a certain part by weight: 0.55-0.65 parts of MnSO4H2O, FeSO4,7H2O, CuSO4,5H2O, KI, and Na2SeO3,5H2O, 21.5-23.5 parts of CoCl2,6H2O, MgO, NaHCO3, Na2SO4, vitamin A, vitamin D, vitamin E, and stone dust, other parts of Ca(HPO4)2, monensin, bone powder, urea, hawthorn, malt, dried orange peel, fleece-flower root, schisandra chinensis, ligusticum wallichii, black bean and wheat middling. The invention is capable of being widely used as a meat lamb fattening feed additive.

The invention relates to a novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and a culture method. The fermentation unit of the monensin can be increased, the fermentation period can be shortened and the fermentation cost can be reduced by use of the formula of the culture medium and a fermentation control process, and moreover, the source of raw and auxiliary materials is avoided from environmental influence to the utmost extent, the sufficient supply of the raw and auxiliary materials is guaranteed, and then stable high-efficiency production of the monensin is realized.

The invention discloses a meat sheep fine complementary particle, relating to feed for sheep. The meat sheep fine complementary particle comprises corn, DDGS (Distillers Dried Grains with Soluble), wheat middling, whitewashing bran, molasses, rapeseed meal, maize peel, castor bean meal, meat and bone meal, compound protein, calcium hydrophosphate, middling flour, dynamite, salt, baking soda, magnesium oxide, lysine, mirabilite, antioxidant, choline, cattle multiple vitamin, sweetener, monensin, strong frankincense and fine limestone. The meat sheep fine complementary particle has the characteristics of abundant nutrition and good palatability; and according to the characteristic of rumen microorganism growth, digestion is accelerated, and the use ratio of the feed is improved. When the meat sheep fine complementary particle is used, various crop straws, wickers and leaves are cooperated and mixed for feeding; and the ratio of the feed to meat is (2.5-3):1, growth speed is high, cost is lowered, and the benefit of food culturists is improved.

The invention relates to a total mixed ration in white goat fattening stage and a producing method thereof. The total mixed ration in white goat fattening stage compriseds coarse fodder, concentrate supplement and water, wherein the quality ratio of the mentioned coarse fodder, concentrate supplement and water is 5. 5:4. 5:2- 3. The mentioned coarse fodder is composed of silage, green forage, hay and straw feed, and the mentioned concentrate supplement consists of the combination of corn, bean pulp, cottonseed meal, brewer's grain, calcium hydrophosphate, salt, vitamin complex, trace elements and monensin. For the mixed ration produced through the invention, the coarse fodder and concentrated feed are mixed evenly with comprehensive and balanced nutritional ingredients avoiding picky eating of mutton sheep to effectively decrease the nutritional and metabolic diseases with the occurrence rate reduced by 35%, which is suitable for application in large-scale farm of white goat fattening stage and will improve economic benefit of cultivation.

The concentrated feed additive for beef cattle and mutton sheep consists of chalcanthite 0.3-0.6 wt%, ferrous sulfate monohydrate 0.3-0.6 wt%, manganese sulfate monohydrate 0.6-1 wt%, zinc sulfate monohydrate 2.6-3.0 wt%, I 1.5-1.8 wt%, Se 0.5-0.8 wt%, Co 1-1.4 wt%, magnesia 6.8-7.1 wt%, vitamin A 0.3-0.5 wt%, vitamin D3 0.1-0.2 wt%, vitamin E 0.6-1 wt%, monensin 1.8-2.2 wt%, urease inhibitor 0.4-0.6 wt%, Chinese medicinal stone 28-35 wt%, and starch 45-55 wt%. The concentrated feed additive for beef cattle and mutton sheep can provide vitamins, minerals, trace elements, etc comprehensively to promote the growth of beef cattle and mutton sheep.

The invention belongs to the technical field of animal feed processing and particularly relates to total mixed ration fodder for mutton sheep at the fattening stage. According to the total mixed ration fodder, alfalfa, corn, distillers' grains, soybean meal, malt sprout, corn fiber, cottonseed meal and molasses are mixed and smashed and then are sieved by a 40-mesh sieve, and mixed powder A is obtained; stone powder, calcium hydrophosphate, bentonite, baking soda, sodium chloride, sodium sulfate, montmorillonite, ammonium chloride, monensin, 0.5% of compound premix feed and the mixed powder A are put in a mixer to be mixed and conditioned, the conditioned material is granulated, granular fodder is obtained, packaged and graded, and a finished product is obtained. By comparison with a conventional feeding and fattening method, the marketing time of fattening lambs fed on the prepared fodder is advanced by 15-25 days, the mutton sheep averagely grow 300 g or more every day and the daily average feed intake is 2.5 jin in the process when the fattening lambs are fattened from 17.5 kg to 65 kg or higher, so that the cost of a breeding farmer is substantially reduced, and the benefits are remarkable.

The invention relates to feed for calves in early weaning period. The feed is characterized by containing the following raw materials in percentage by weight: 40 to 60 percent of steamed corn flakes, 5 to 15 percent of extruded soybean meal, 5 to 10 percent of soybean meal, 5 to 10 percent of full-fat soybean, 2 to 6 percent of zein powder, 0 to 10 percent of wheat bran, 0 to 7 percent of sunflower meal, 5 to 10 percent of whey powder, 2 to 6 percent of skimmed milk powder, 1 to 3 percent of glucose, 1 to 2 percent of calcium hydrogen phosphate, 0.2 to 0.8 percent of table salt, 1 to 2 percent of stone powder, 0.2 to 0.3 percent of 0.2 wt% bovine E-mastitis formulation, 0.1 to 0.3 percent of methionine, 0.1 to 0.3 percent of yeast culture and 0.01 to 0.02 percent of monensin. The feed has the advantage that the proportion and level of plant proteins in calf feed are optimized according to the physiological development and immune response characteristics of calves, and the feed has the characteristics of having total nutrition and good palatability, being easy to be digested, saving milk, reducing the calf raising cost and increasing the calf survival rate.

The invention discloses a method for producing ethanol through fermentation of a starch bearing material via a composite fungistat raw material. The method comprises the following steps: crushing the starch bearing material into powder; preparing powder slurry with a dry matter mass concentration of 30 to 33% from the crushed starch bearing material, water and a clear liquid; pretreating the powder slurry by adding acidic protease, amylase and a composite fungistat into the powder slurry, adjusting a pH value to 4.0 to 4.5 and stirring the powder slurry so as to prepare a pretreated mash, wherein the composite fungistat contains penicillin and monensin; and adding glucoamylase, yeast and nutritive salt into the pretreated mash and carrying out fermentation. A mature fermented mash prepared by using the method has high alcohol concentration and high starch liquor yield; application of the composite fungistat enables the concentrations of lactic acid and acetic acid in raw material fermentation to be reduced, mixed fungus pollution to be reduced and the alcohol concentration and the starch liquor yield of the mature fermented mash to be further improved.

The invention discloses a preparation method of a complete pellet sheep feed. The complete pellet sheep feed comprises black locust leaves, selenium-enriched alfalfa, peas, banana stems and leaves, edible mushroom residue, a traditional Chinese medicine additive, monensin, rumen by-pass amino acid, yeast powder, compound trace elements, multivitamins, molasses, soybean meal, calcium hydrogen phosphate, and edible salt; the preparation method includes steps of crushing, fermentation, drying, granulation, coating and the like. The complete palletized sheep feed is rich and balanced in nutritionand wide in sources of raw materials, the preparation method is simple and convenient, and short in preparation time, and the volume ratio of the feed is reduced; secondly, the moisture content in thefeed is well controlled in the preparation process, the finished feed product is not easy to mildew, and the survival rate of live bacteria in the feed is also improved. The shelf life is extended, the feed is easy to transport and store, the cost of sheep breeding is reduced, and the method has a good prospect.

The invention discloses a preparation method of a monensin premix. The preparation method comprises the following specific steps: preparing cinnamon streptomyce strain; seed flask culture and seed tank culture are performed; when the age of the seed is 20-24h, the pH of a culture solution is 6.6-7.1, the biomass is above 10%, and microscopic examination shows that mycelia are thick and reticular, are stretched and darkly stained and are free of foreign bacteria, performing fermentation culture is performed; when the residual oil amount is below 0.7%, the biomass is above 40%, the mycelium concentration is 40-50%, and the monensin titer is not lower than 40000U / mL, fermentation is finished; monensin is extracted by a one-step spray drying method, wherein the prepared finished monensin product has the monensin content of above 20% and the grain rate of above 75%. According to the preparation method, the fermentation process is simple, so that the fermentation unit can be effectively increased and the production cost can be reduced; usages of an animal-derived nitrogen source and a chemical organic solvent are avoided.

The invention discloses a method of detecting monensin by immunomagnetic bead purification-enzyme-linked immunoassay and a special monoclonal antibody used therein. The accession number of a monensin monoclonal antibody hybridoma cell strain MON (hybridoma cell strain MON for short) provided by the invention is CGMCC No.8952. The monoclonal antibody secreted by the hybridoma cell strain MON also belongs to the scope of protection of the invention. Immunomagnetic beads obtained by coupling the monoclonal antibody and magnetic beads also belong to the scope of protection of the invention. The immunomagnetic beads are used for enriching and purifying MON molecules in a sample, the concentrated sample is detected by indirect competitive ELISA finally, and the MON content to be measured is calculated. The method can detect MON samples exceeding the limit of detection (1 ng / mL) of common immunodetection methods, and effectively improves the detection sensitivity.

The invention relates to a calf appetite stimulating feed for reducing weaning stress and increasing daily gain of calves, namely Duniule, belonging to the field of dairy cattle breeding feeds. The calf appetite stimulating feed is prepared by the steps of carrying out steam tempering on the following raw materials in parts by weight: 20-30 parts of corns, 30-40 parts of bean pulp, 6-10 parts of corn germ pulp, 5-12 parts of soybean hull, 0.8-1.6 parts of table salt, 2-3 parts of mountain flour, 1.5-2.5 parts of calcium hydrogen phosphate, 0.5-1.0 part of baking soda,0.2-0.4 part of potassium chloride, 1-2 parts of lactose, 0.06-0.08 part of compound vitamins, 0.3-0.5 part of compound microelements, 1-2 parts of a yeast culture, 0.1-0.3 part of immunopolysaccharide and 0.02-0.05 part of monensin, granulating, mixing the raw materials with preprocessed raw materials, and spraying molasses. The calf appetite-stimulating feed is specifically prepared by the following steps: mixing the raw materials in a formula, carrying out tempering for 20 seconds, maintaining the steam temperature at 85-92 DEG C, granulating, standing for 55-65 seconds in a holder, and carrying out cooling formation in a cooling room so as to form granules A with the diameters of 3.0mm-3.3mm; softening the corns in water, carrying out high-temperature tabletting, and cooling so as to prepare tabletted corns B; softening barley in water, carrying out high-temperature tabletting, and cooling so as to prepare tabletted barley C; and finally mixing 50-60 parts of the granules A, 20-30 parts of the tabletted corns B and 10-15 parts of the tabletted barley C according to the formula, adding 3-8 parts of the molasses, and spraying and mixing for 45 seconds, so as to obtain a granule-tabletted cereal combined feed, namely calf appetite stimulating feed-Duniule.

The invention discloses a method for detecting monensin and a dedicated enzyme-linked immune kit. The enzyme-linked immune kit comprises a monensin special antibody, and a coating antigen and an enzyme label; the coating antigen is a coupling compound of the half antigen and carrier protein of the monensin or an antiantibody; the enzyme labelis an enzyme-labeled antiantibody or the half antigen of the enzyme-labeled monensin; when the coating antigen is the coupling compound of the half antigen and the carrier protein of the monensin, the enzyme label is the enzyme-labeled antiantibodie; when the coating antigen is the antiantibody, the enzyme label is the half antigen of the enzyme-labeled monensin. The enzyme-linked immune kit in the invention has the advantages of simple structure, convenient operation, low price, and convenient carrying; the detection method is high efficient, accurate and simple; and the kit is suitable for screening mass samples on site. The kit in the invention plays an important part in detecting the monensin.

Disclosed are a Coryneform bacterium having resistance to an antibiotic, monensin, and producing L-lysine, and a process for producing L-lysine by a direct fermentation, which comprises culturing said microorganism in a fermentation medium, accumulating L-lysine in the resulting culture broth, and recovering L-lysine therefrom.

The invention belongs to the technical field of veterinary drug residue analysis and discloses a high performance liquid chromatogram pre-column derivatization reaction analysis method for a hydrogen substitution derivatization reaction of carboxyl compounds of polyetherionophores. Carboxyl groups contained in polyetherionophores react with a fluorescent reagent of 1-(bromoacetyl) pyrene, and the products have extremely-high fluorescence. The following reaction conditions are optimized: a 18-crown ether-6 solution is used as a phase transfer catalyst; a 1-(bromoacetyl) pyrene solution is used as a derivatization reagent; the reaction is conducted in a water bath at 30-70 DEG C for 150 min; after being cooled, the products are detected through a high performance liquid chromatograph. The excitation wavelengths of the derivatives are 360 nm; the derivatives have the highest fluorescence intensity when the emission wavelengths of the derivatives are 420 nm; the minimum detectable amount is 50 Mug / L; the derivatives show linear response to peak areas within the range of 200-1600 [mu]g / L; the result reproducibility is high; the variable coefficient of 3 concentrations and 15 repetitions is smaller than 7.8%; all indexes can meet the residue analysis requirements of the three polyetherionophores of monensin, salinomycin and lasalocid.

The invention relates to a preparation method of a composite plant powder for minimizing methane output of rumen culture. Garden balsam, Atractylodes lancea and Yedo violet which are dried to constant weight are crushed according to a certain proportion to prepare the composite plant powder. By the addition of 0.2%, 0.5% and 1.0% of the powder, the output of methane of rumen culture within 24 hours are respectively reduced by 83.62%, 96.89% and 98.67% in comparison with a control group. In comparison with the methane output in a monensin treatment group, the methane output by the addition of 0.5% and 1.0% of the powder is respectively reduced by 23.69% and 32.31%. In the meantime, the powder can also increase the output of total volatile aliphatic acid and reduce propionic / acetic acid ratio, and has a better effect than monensin. It shows through results that the composite plant powder has a substantial effect of reducing methane as well as performance for improving rumen fermentation, and can replace antibiotics to regulate and control rumen fermentation and control methane generation. Therefore, health and environmental problems derived from antibiotics can be effectively eliminated.

The invention discloses a premix compound for fattening beef cattle. The premix compound comprises the components: 2.1-5.2% of compound trace element, 0.15-0.9% of compound vitamin, 93.9-95.4% of carrier, 0.25% of sulfur, 0.075% of aureomycin and 0.375% of monensin. The invention further discloses a premix compound proportion for different fattening periods of snowflake beef and a preparation method of the premix compound.

The present invention discloses a hystrix hodgsoni meat quality improving nutritional feed which belongs to the technical field of hystrix hodgsoni breeding. The nutritional feed is composed of the following materials (parts by weight): 100-120 corn flour, 80-90 peanut bran, 70-80 whole milk powder, 20-25 fresh water fish meal, 2-3 livestock glutamic acid, 1-2 multi-vitamins, 0.5-1 monensin, 0.5-1 massive gainer, 2-4 Scutellaria baicalensis, 2-3 orange peels, 2-4 Gardenia jasminoides, 2-4 folium cortex eucommiae and 1-2 salt. The nutritional feed enables the healthy growth of hystrix hodgsoni and supplements hystrix hodgsoni with plenty of energy, increases lean meat percentage and improve meat quality of hystrix hodgsoni.

The invention provides a mutton sheep fattening period concentrate supplementing material formula.The mutton sheep fattening period concentrate supplementing material is prepared from, by weight, 15-25% of corn, 10-15% of wheat bran, 4-6% of wheat middling, 8-12% of germ rice dregs, 6-10% of millet oil bran, 2-4% of peanut meal 11-15% of cottonseed meal, 2-5% of rapeseed meal, 7-9% of whitewashing fiber, 7-9% of malt sprouts, 3.0-3.5% of molasses, 0.5-0.8% of urea, 1.0-1.8% of baking soda, 0.4-0.55% of magnesium oxide, 0.6-0.85% of calcium hydrophosphate, 1.5-2.5% of mountain flour, 0.8-1.1% of salt, 0.4-0.6% of a composite premix compound and 0.01-0.03% of monensin with the purity being 20%.According to the mutton sheep fattening period concentrate supplementing material, various grain and oil processing by-products with the high nutritive value and low price are adopted, nutrients are balanced, the nutrient requirement for the mutton sheep fattening period is sufficiently met, and low cost of feed is achieved.

The invention discloses a compound Chinese herbal medicine condensed feed additive for meat goats in Karst mountainous areas in the southwest. The additive has a reasonable ratio and comprehensive nutrition, and is suitable for growth of meat goats in Karst mountainous areas in the southwest. The compound Chinese herbal medicine condensed feed additive for goat comprises the following ingredientsby weight: 0.25-0.55% of blue vitriol, 0.2-0.65% of ferrous sulphate monohydrate, 0.5-0.9% of manganese sulfate monohydrate, 2.5-3.0% of monohydrate zinc sulphate, 1.4-1.9% of iodine, 0.6-1.0% of selenium, 1.0-1.4% of cobalt, 7.1-7.5% of magnesium oxide, 0.3-0.35% of vitamin A, 0.1-0.2% of vitamin D3, 0.6-1% of vitamin E, 1.5-2.0% of monensin, 5.0-5.5% of compound Chinese herbal medicine, 25-35% of shell powder and 40-50% of corn flour, wherein the compound Chinese herbal medicine comprises 5% of epimedium, 25% of sweet wormwood, 45% of honeysuckle flower and 25% of green tea. The additive has a reasonable ratio, comprehensive nutrition, reliable quality, so that the additive can enable meat goats to grow fast, effectively reduce goat alimentary canal pathogen, remove and adsorb mycotoxinin forage grass, and prevent the forage grass from moulding.

The invention relates to the field of feed additives and discloses a feed addition material, a preparation method thereof and a feed additive. The feed addition material comprises the ingredients in parts by weight: 1 to 20 parts of beta-cyclodextrin, 10 to 80 parts of monensin and 1 to 10 parts of glycyrrhizic acid. The invention also provides the feed additive, which comprises the feed additionmaterial, a filling agent, a bonding agent and a disintegrating agent. The feed addition material uses the beta-cyclodextrin as coating materials, the influence on the animal palatability caused by the monensin is avoided, in addition, the glycyrrhizic acid is added for prompting the absorption of the monensin in animal bodies and the persistent medicine effect taking, the animal growth can be more perfectly promoted, the coccidiosis is prevented and treated, and the feed addition material can be applied to the preparation process of feed additives.

The invention discloses an efficient total mixed ration for fattening Haimen goat and a production method thereof. The efficient total mixed ration is prepared from coarse feed, concentrated feed, additives and water, wherein the mass ratio of the coarse feed to the concentrated feed is 6:4, and the additives comprise microelements for mutton sheep, compound vitamins and monensin, wherein the weight of the microelements for mutton sheep is 0.1 of the sum of the weights of the coarse feed and the concentrated feed, the weight of the compound vitamins is 0.01% of the sum of the weights of the coarse feed and the concentrated feed, the weight of the monensin is 0.07% of the sum of the coarse feed and the concentrated feed, and the mass content of water is 30-59%. According to the efficient total mixed ration, the feed intake can be effectively increased, nutritional metabolic diseases are reduced, and the weight of the goat can be increased.

The invention discloses a method for improving the detection rate of cytokines secreted by peripheral blood leukocytes. The method comprises the following steps: S1, taking whole blood and adding a culture solution to dilute the whole blood; S2, adding phorbol ester and ionomycin calcium salt, carrying out culturing for 6 hours, and adding monensin in the later 4 hours of the culture so as to obtain cells to be detected; S3, adding a cell surface labeled antibody into the cells to be detected for staining of the cells to be detected, dissolving red blood cells with a whole blood sample, carrying out washing with a staining buffer, adding polyformaldehyde for lucifugal immobilization, adding a membrane permeating buffer for membrane permeation, adding bovine serum albumin and mouse serum for blocking, adding a cytokine antibody for staining, carrying out washing with membrane permeating buffer, and carrying out washing with a staining buffer to obtain stained cells; and S4, carrying outdetection with a flow cytometer. The method of the invention has a wide application range and high ratio of cytokine secreting cells, and experimental data and control data have statistical significance.