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Method for detecting monensin and its special ELISA reagent kit

An enzyme-linked immunosorbent reagent, monensin technology, which is applied to measurement devices, cells modified by introducing foreign genetic material, instruments, etc., can solve the problems of high cost, limited promotion and application, cumbersome processing and measurement operations, etc. Easy to use, efficient detection method, convenient and easy to implement

Inactive Publication Date: 2008-12-03
BEIJING WANGER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, high performance liquid chromatography, gas chromatography-mass spectrometry and other instrumental methods are mainly used for the detection of monensin drug residues in animal tissues. However, the sample pretreatment and determination of instrumental methods are cumbersome and expensive, which limits their popularization and application.

Method used

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  • Method for detecting monensin and its special ELISA reagent kit
  • Method for detecting monensin and its special ELISA reagent kit
  • Method for detecting monensin and its special ELISA reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Preparation and detection of a kit using the conjugate of monensin hapten and carrier protein as the coating source

[0028] 1. The detection principle of the kit using the conjugate of monensin hapten and carrier protein as the coating source is as follows:

[0029] When the microwell strip is pre-coated with monensin-conjugated antigen, after adding the sample solution or standard solution, immediately add the enzyme-labeled secondary antibody, and then add the monensin-specific antibody solution, the remaining monensin in the sample The monensin drug and the monensin-coupled antigen coated on the ELISA plate compete with the monensin-specific antibody, and the enzyme-labeled anti-antibody is added for amplification, and the color is developed with a chromogenic solution. The absorbance value of the sample is related to the monensin The content of monensin drug is negatively correlated, and the residual amount of monensin in the sample can be obtained by co...

Embodiment 2

[0097] Embodiment 2, test kit sensitivity, accuracy and shelf life test

[0098] 1. Standard product precision test:

[0099] From the kits of different batches (01 batches, 03 batches, and 06 batches) prepared in different time periods in step 3 in Example 1, 10 kits were respectively extracted, and 20 kits were respectively extracted from the enzyme-linked plate of each kit. measure the absorbance value (OD value) of 9 μg / L monensin standard solution, and calculate the coefficient of variation. The results are shown in Table 1, indicating that the coefficients of variation of each batch of 10-time standard products are all between 5.4% and 13.1%, meeting the requirement that the precision is less than or equal to 25%.

[0100] Table 1. Standard repeatability test (CV%)

[0101]

[0102] 2. Sample precision and accuracy test

[0103] 1. Sample precision test:

[0104] Add monensin to pig muscle and pig liver tissue without monensin to make the final concentration 10 μg...

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Abstract

The invention discloses a method for detecting monensin and a dedicated enzyme-linked immune kit. The enzyme-linked immune kit comprises a monensin special antibody, and a coating antigen and an enzyme label; the coating antigen is a coupling compound of the half antigen and carrier protein of the monensin or an antiantibody; the enzyme labelis an enzyme-labeled antiantibody or the half antigen of the enzyme-labeled monensin; when the coating antigen is the coupling compound of the half antigen and the carrier protein of the monensin, the enzyme label is the enzyme-labeled antiantibodie; when the coating antigen is the antiantibody, the enzyme label is the half antigen of the enzyme-labeled monensin. The enzyme-linked immune kit in the invention has the advantages of simple structure, convenient operation, low price, and convenient carrying; the detection method is high efficient, accurate and simple; and the kit is suitable for screening mass samples on site. The kit in the invention plays an important part in detecting the monensin.

Description

technical field [0001] The invention relates to a method for detecting monensin and a special ELISA reagent kit thereof. Background technique [0002] Monensin is a polyether antibiotic widely used in the treatment of animal diseases. Its main mechanism of action is to interfere with the normal exchange of sodium and potassium ions in coccidian cells, allowing a large amount of sodium ions to enter the cells, resulting in The concentration of sodium ions in the cells is high, the osmotic pressure rises, and more water enters the coccidia. In order to eliminate the excess sodium in the cells, the coccidia must use energy to pump out the excess sodium, and eventually the limited energy is used up. ; Then the sodium and potassium ion exchange was interrupted and stopped, and the cells swelled, deformed, ruptured, and disintegrated. Monensin can also affect the energy metabolism in the rumen of ruminants, improve rumen fermentation, increase the output ratio of propionic acid a...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577G01N33/535C12N5/12
Inventor 沈建忠何方洋肖希龙冯才伟万宇平马孝斌赵正苗罗晓琴冯月君
Owner BEIJING WANGER BIOTECH
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