207results about How to "Low pre-processing requirements" patented technology

This invention discloses one EPSPS gene enzyme immune agent case and its use method in the anti-herbicide bean, wherein, the case comprises anti-EPSPS multi-clone antigen, enzyme board with the antigen, enzyme antigens for two, gene switch bean standard product, non-transfer gene bean standard, intense wash liquid, develop liquid and terminal liquid. This invention method comprises the following steps: cloning CP4-EPSPS gene from the gene switch bean expressed in the bacillus coli; then through protein purification complex property to regroup EPSPS protein antibody immune animal to get special single clone antigen and multi-clone antigen; establishing double antigen clamp enzyme immune system test sample to determine its EPSPS protein content.

The invention discloses a simple, fast and sensitive method for detecting a plasticizer (phthalate ester) in plastic, which is based on ion mobility spectrometry and detects phthalate ester in an extracting solution using a fast ion mobility spectrometry detector in combination with a simple organic solvent extraction pretreatment method. The measurement method is simple, fast and reliable. The analysis time of the method is only 0.5min, the requirement on a sample pretreatment is low, the detection time is short, the sensitivity is high, and the detection limit can be up to 1ppb.

The invention discloses a method for detecting a sulfanilamide medicine and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a sulfanilamide medicine monoclonal antibody and the sulfanilamide medicine. The sulfanilamide medicine monoclonal antibody is secreted by the hybridoma cell line SAs of a sulfanilamide monoclonal antibody, whose preservation number is CGMCC No. 3393. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the sulfanilamide medicine in products (especially milk, pork, chicken, eggs, honey, fish, shrimp and the like) eaten by animals or people. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the sulfanilamide medicine monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.

The invention provides an elisa kit for detecting cephalo-type medicine, containing: an ELIAS plate peridium of which is provided with peridium source, an enzyme label, cephalo-type medicine specific antibody working solution (contained when the peridium on the ELIAS plate is antigen and the enzyme label is enzyme label anti antibody or when the peridium on the ELIAS plate is anti antibody and theenzyme label is enzyme label antigen), ceftiofur standard solution, substrate developer, stop solution, concentrated cleaning solution and concentrated complex solution. The invention also disclosesa method applying the elisa kit for detecting cephalo-type medicine, including: firstly sample preprocessing is carried out, then the elisa kit is used for detection, and finally the detection result is analyzed. The elisa kit provided by the invention can be applied to detecting residual quantity of cephalo-type medicine in samples such as animal tissue (chicken, pork, fish and shrimp) and milk, etc, the operation is easy, the cost is low, the sensitivity is high, and the elisa kit is applied to on-site supervision and numerous sample screening.

The invention discloses an enzyme immune agent box for detecting lake drugs of animal foodstuff; it also provides a method for using the agent to detect the lake drugs residue. The agent box comprises: enzyme mark plate which coats lake drugs antigen or antibody, lake drugs mouse monoclonal antibody or polyclonal antibody working solution, enzyme mark antibody or enzyme mark lake drugs antigen, lake drugs standard solution, base material color developing solution, compression cleaning liquid, ending solution and compression twin solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The invention provides an enzyme-linked immunosorbent kit for inspecting sulfa drugs, comprising an ELISA plate which is coated with coating antigen, an enzyme label, sulfa drug specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), sulfamethoxy-isoxazole standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the sulfa drugs, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the residual amount of the sulfa drugs in animal tissues(chicken, pork, fish and shrimp), honey, eggs, milk, feeds and other samples, the operation is simple, the cost is low, the sensitivity is high and the enzyme-linked immunosorbent kit can be monitore d on-site and is applicable in screening mass samples.

The invention provides a detecting furanketone metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, furanketone metabolite specific antibody or furanketone metabolite derivative antibody, furanketone metabolite standard solution or furanketone metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washing solution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting furanketone metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the furanketone metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.

The invention discloses a magnetic particle chemiluminescence immunoassay kit of free thyroxine (FT4), belonging to the medical field of immunoassay. The kit comprises: 1) free thyroxine series calibrator; 2) magnetic particle solution enveloped by anti-fluorescein isothiocyanate monoclonal antibody; 3) free thyroxine antibody solution marked by fluorescein isothiocyanate; 4) free thyroxine markedby horse radish peroxidase; 5) chemiluminescence substrate liquid; and 6) concentrated washing liquid. The invention also discloses a preparation method of the kit. The kit in the invention has advantages of simpleness, convenience, rapidness, sensitivity, stability and the like.

The invention provides a chemoluminescence immunoassay measuring kit and a preparation method thereof for hepatitis B core antibody magnetic particles. The kit comprises a hepatitis B core antibody calibration sample, magnetic particles coated by a hepatitis B core antigen, an enzyme marker, a chemoluminescence substrate and a concentrated washing solution. The invention effectively utilizes a magnetic particle solid separation system and a chemoluminescence immunoassay technology to detect the hepatitis B core antibody. The kit has the advantages of simple and fast detection, high sensitivity, strong specificity, stability, and the like, can well satisfy the requirement of clinic diagnosis and is suitable to be effectively generalized and applied in industry.

The invention discloses a magnetic-particle separation chemiluminescence immunodetection method for glial fibrillary acidic protein (GFAP) of a human body. A kit comprises a calibration product, a quality control product reagent A, a reagent B, concentrated cleaning liquid and luminescent substrate liquid, wherein the calibration product is antigen containing a series concentrations of glial fibrillary acidic proteins and is used for establishing standard curves; the quality control product is prepared by buffering solution of antigens containing certain-concentration glial fibrillary acidic protein; the reagent A is antibody solution containing certain-concentration glial fibrillary acidic protein and labeled by magnetic particles; the reagent B is antibody solution containing certain-concentration glial fibrillary acidic protein and labeled by the alkaline phosphatase; the concentrated cleaning liquid is used for preparing cleaning liquid; the luminescent substrate liquid is luminescent substrate solution catalyzed by alkaline phosphatase (ALP). The magnetic-particle separation chemiluminescence immunodetection method disclosed by the invention has the beneficial effects that thesignal intensity and the sensitivity of immunoreaction are greatly improved, low-content substances also can generate strong chemiluminescence signals when in immunobinding, and a method with more accuracy, precision, convenience and simpleness is provided for detecting the glial fibrillary acidic protein of the human body.

The invention relates to the medical field of immunoassay, particularly provides a measuring kit of the chemiluminescence immunoassay of a CA125 magnetic particle. The kit of the invention comprises: 1) a CA125 working calibrator; 2) the mixed solution of the magnetic particle coated with a CA125 monoclonal antibody and the CA125 monoclonal antibody marked by biotin; 3) streptavidin marked by horse radish peroxidase; 4) a chemiluminescence substrate; and 5) a reaction tube. Further, the method for preparing the kit according to the invention comprises the following steps: 1) the working calibrator is prepared by CA125 pure products; 2) the mixed solution of the magnetic particle coated with the monoclonal antibody and the monoclonal antibody marked by the biotin is prepared; 3) the horse radish peroxidase is used for marking the streptavidin; 4) the chemiluminescence substrate is prepared; 5) the working calibrator, the mixed solution, the enzyme markers, the chemiluminescence substrate and the reaction tube are sub-assembled; and 6) assembly is carried out to obtain a finished product. The kit of the invention has the advantages of simpleness and convenience, fastness, sensitivity and stability and the like.

The invention provides a detecting Furacilin metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, Furacilin metabolite specific antibody or Furacilin metabolite derivative antibody, Furacilin metabolite standard solution or Furacilin metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washing solution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting Furacilin metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the Furacilin metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.

The enzyme-linked immunological kit for detecting 3-methyl quinoxaline-2-carboxylic acid (MQCA) as the quinoxaline medicine residue marker includes specific antibody for detecting MQCA and standard enzyme plate, standard enzyme secondary antibody and other enzyme-linked immunological agent coated with MQCA derivative and carrier protein coupler. The present invention is suitable for detecting mass samples, and has main reagent in liquid form, fast and convenient detecting process, high specificity, high sensitivity, high precision, high accuracy and other features.

The invention relates to the medical field of immunoassay, and concretely provides a dissociate human chorionic gonadotrophin beta-subunit magnetic particle chemiluminescence quantitative assay kit having the advantages of simplicity, rapidness, high sensitivity, wide linear range and good stability, and its preparation method. The method combines a magnetic particle immune separating technology to apply an enzymatic chemiluminescence substrate on the basis of enzyme-linked immunosorbent assay, and allows an optical signal generated by the detection of the luminescence substrate to substitute a chromogenic substrate in enzyme immune assay, so the method has the advantages of substantially improved sensitivity, simple operation and wide practicality, can be applied to open semi-automatic chemiluminescence detectors, can also be applied to full-automatic measure systems, and can realize batch and fast detection, low use cost, and easy popularization and application.

The invention provides an ELISA (enzyme linked immunosorbent assay) kit for detecting carbofuran. The ELISA kit comprises an ELISA plate coated with a carbofuran conjugated antigen, a carbofuran monoclonal antibody, an enzyme labeled antibody, a carbofuran standard solution, a substrate color development solution, a stop buffer, a cleaning solution and a re-dissolution solution. The invention further discloses a method for applying the ELISA kit to detection of the carbofuran. The method comprises the following steps: a sample is pretreated firstly, then the kit is used for detection, and a detection result is analyzed finally. The ELISA kit can be used for detecting the content of the carbofuran in vegetables and tea leaves, the operation is simple and convenient, the cost is low, the sensitivity is high, on-site supervision can be realized, and the kit is suitable for screening of a large quantity of samples.

The invention provides an enzyme immune agent box for detecting chloromycetin drugs of animal foodstuff which comprises: enzyme mark plate which coats chloromycetin drugs antigen or antibody, chloromycetin drugs mouse monoclonal antibody compression solution, chloromycetin drugs standard solution, enzyme mark material, base material color developing solution, compression cleaning liquid, ending solution and compression twin solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The invention provides an enzyme immune agent box for detecting sulpha drugs of animal organization, which uses enzyme immune method to detect the preprocessed animal organization, honey, urine, milk. The enzyme immune agent box comprises: enzyme mark plate which coats sulpha drugs antigen or antibody, sulpha drugs mouse monoclonal antibody working solution, enzyme mark antibody or sulpha drugs antigen solution, sulpha drugs standard solution, base material color developing solution, compression cleaning liquid, ending solution and compression extracting solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The invention discloses a method for detecting melamine and a special enzyme-linked immunosorbent assay kit thereof. The enzyme-linked immunosorbent assay kit for detecting the melamine comprises a hapten and a specific antibody of the melamine; and the specific antibody is the polyclonal antibody or the monoclonal antibody of the melamine. The kit adopts the high specific monoclonal antibody of the melamine, thereby ensuring the reliability of test results; the test results show that the kit is characterized by high specificity, high sensitivity, high precision and high accuracy; main reagents of the kit all adopt the form of working liquid, thereby having convenient use and low cost. The method for detecting the melamine by using the kit has simple operation, low requirements on the pre-treatment of samples and can rapidly detect large quantities of samples. Therefore, the use of the method for detecting by using the enzyme-linked immunosorbent assay kit can monitor on-site and be applicable to qualitative and quantitative screening of a large number of samples.

The present invention belongs to the field of iron and steel surface treating technology, and is especially one kind of nanometer anticorrosive paint, its preparation process and steel rail coated with the paint. The nanometer inorganic paint consists of filming material 20-60 wt%, nanometer pigment 3-8 wt%, antirust assistant 5-15 wt%, other assistants 0.025-4 wt%, and deionized water 30-70 wt%. The nanometer inorganic paint has excellent painting performance, high mechanical strength, high anticorrosive performance, high weather resistance, capacity of forming thin smooth coating with certain conducting performance, and other advantages.

The present invention discloses a method for detecting the erythromycin compounds and an enzyme linked immunoreaction reagent kit thereof which is specialized for the method. The enzyme linked immunoreaction reagent kit comprises an erythromycin specific antibody, a coatingen and an enzyme label. The erythromycin specific antibody is a monoclone antibody or polyclonal antibody of the erythromycin. The erythromycin compound is at least one in erythromycin, erythromycin thiocyanate and erythromycin ethylsuccinate. The erythromycin is erythromycin A. The enzyme linked immunoreaction reagent kit of the invention has the advantages of simple structure, convenient use, low cost, convenient carriage, high efficiency of the detecting method, accuracy and easiness. The on-spot monitoring can be executed. Furthermore the enzyme linked immunoreaction reagent kit is suitable for the qualitative and quantitative sieving of considerable samples, and can exert an important function in the detection to the erythromycin.

The invention relates to a magnetic particle chemiluminescence immune assay kit of tumor marker AFP (alpha fetal protein) and a detection method of the magnetic particle chemiluminescence immune assay kit, which belongs to the technical field of the immune detection and analysis. An AFP monoclonal antibody marked by fluorescein isothiocyanate (FITC) and a monoclonal antibody marked by alkaline phosphatase (AP) are combined with an antigen to form a sandwiched immune compound of an FITC marked antibody-antigen-AP marked antibody, and the sandwiched immune compound is similar to a sandwich structure. Then the magnetic particles which are connected with the anti-FITC antibody are added, the specific binding of the anti-FITC antibody and the FITC enables antigen-antibody complex to be connected onto the magnetic particles, the magnetic particles are directly precipitated in an external magnetic field, and the compound formed in the immune reaction manner can be separated from other non-combined substances without centrifuging. According to the kit, the chemiluminescence is combined with the magnetic particles, a reaction system which is approximate to the homogenous phase is provided, and compared with the prior art, the kit has the advantages of high sensitivity, wide linear range, high speed and the like; moreover, the product cost is greatly reduced, and the application prospect on the aspects such as the clinical inspection is promising.

The invention discloses a preparation method of magnetic particle chemiluminescence detection kit for testing human body anti-mullerian hormone content. The kit contains anti-mullerian hormone R1 reagent, anti-mullerian hormone R2 reagent, a magnetic separation reagent, standard product liquid series and chemiluminescence substrate solution; the anti-mullerian hormone R1 reagent is anti-mullerianhormone monoclonal antibody diluent marked by fluorescein isothiocyanate, the anti-mullerian hormone R2 reagent is anti-mullerian hormone antibody diluent marked by alkaline phosphatase, the magneticseparation reagent is magnetic particle diluent coated by anti-fluorescein isothiocyanate monoclonal antibody, the anti-mullerian hormone standard product liquid is formed by synthesized anti-mullerian hormone and buffer liquid, and the chemiluminescence substrate solution is Tris-HCL buffer liquid which is catalyzed by alkaline phosphatase and contains dioxane. The method greatly improves the signal intensity and sensitivity of immunoreaction, can enable low-content substances to generate a strong chemiluminescence signal when performing immune binding, so as to provide a more accurate, accurate, convenient, rapid and simple method for detection of anti-mullerian hormone.

The invention relates to an ELISA Kit and a detection method for detecting chlorpromazine in the veterinary medicine residual detection analysis technical field. The chlorpromazine ELISA Kit comprises a chlorpromazine standard solution, a developer, a concentrated cleaning solution, a termination solution and a sample diluent. In addition, the chlorpromazine ELISA Kit also comprises a chlorpromazine specificity antibody, an enzyme label plate and an enzyme label, wherein the enzyme label is an enzyme labeling chlorpromazine antigen. The ELISA Kit for detecting chlorpromazine provided by the invention has low requirements on the preliminary treatment of a sample, a simple sample preliminary treatment process and a capability of detecting a large number of samples quickly. The ELISA Kit for detecting chlorpromazine which is characterized in high specificity, high sensitivity, high precision, high accuracy, etc. will contribute much to the detection of chlorpromazine residual.

The invention discloses a new method for detecting melamine. Based on an ion mobility spectrometry technology, by using the ion mobility spectrometry as a detection means and combining a simple solvent extraction pretreatment method, the method has high detection speed and sensitivity and needs only 0.5 minute for analysis and less than 5 minutes for pretreatment; and the detection limit can reach 50ppb.

The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting mercury ions and a manufacturing method of the kit. An elisa plate enveloped by mercury ion envelope antigens, mercury-ion-resisting monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a mercury ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The kit can detect the mercury irons in a trace mode, is used for detecting the mercury irons in the environment, the soil, water, foods, medicines, cosmetics and the like, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit is low in requirement for pretreating samples, simple in treatment process, not only can be used for screening the samples in large batch, but also can perform rapid detection on the samples in small batch, and not only provides technical support for environment and food safety, but also provides effective technological means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.

The invention relates to the medical field of immunoassay, particularly provides a measuring kit of the chemiluminescence immunoassay of a CA153 magnetic particle. The kit of the invention comprises: 1) a CA153 working calibrator; 2) the mixed solution of the magnetic particle coated with a CA153 monoclonal antibody and the CA153 monoclonal antibody marked by biotin; 3) streptavidin marked by horse radish peroxidase; 4) a chemiluminescence substrate; and 5) a reaction tube. Further, the method for preparing the kit according to the invention comprises the following steps: 1) the working calibrator is prepared by CA153 pure products; 2) the mixed solution of the magnetic particle coated with the monoclonal antibody and the monoclonal antibody marked by the biotin is prepared; 3) the horse radish peroxidase is used for marking the streptavidin; 4) the chemiluminescence substrate is prepared; 5) the working calibrator, the mixed solution, enzyme markers, the chemiluminescence substrate and the reaction tube are sub-assembled; and 6) assembly is carried out to obtain a finished product. The kit of the invention has the advantages of simpleness and convenience, fastness, sensitivity and stability and the like.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content ofthe porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is appl icable in screening mass samples.

The present invention discloses a method of detecting colistin, and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit comprises the colistin and specific antibodies of the colistin; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the colistin. The kit adopts the high specificity monoclonal antibodies of the colistin, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low. The operation of the method which adopts the kit to detect the colistin is simple, the detection time is shortened, the operation have low requirements for the pretreatment of the sample, and the method can be used for simultaneously and rapidly detecting the samples in bulk. Therefore, the method which adopts the enzyme linked immunosorbent kit for detection can play a significant role in the detection of the colistin.

The invention discloses a magnetic particle-isolated chemiluminescence immunoassay for detecting Tau protein (TAU). A kit composition comprises a calibration product, a quality control product reagentA, reagent B, concentrated cleanout fluid, and luminescent substrate solution, wherein the calibration product is Tau protein (TAU) antigen in a series of concentration and used for establishing a standard curve; the quality control product is prepared from the buffer solution containing Tau protein (TAU) antigen in a series of concentration; the reagent A is the magnetic particle marked Tau protein (TAU) antibody solution in a series of concentration; the reagent B is the alkaline phosphatase marked Tau protein (TAU) antibody solution in a certain concentration; the concentrated cleanout fluid is used for preparing the cleanout fluid; the luminescent substrate solution is the alkaline phosphatase (ALP) catalyzed luminescent substrate solution. The signal intensity and sensitivity of theimmunoreactions are greatly improved, the strong chemiluminiscence signal can be produced when the low-content substance performs the immune binding, a more accurate, precise, convenient, faster and simpler method is provided for the detection of the human body Tau protein (TAU).