The invention provides a portable
copper ion concentration detection method based on
click chemistry and belongs to the field of
analytical chemistry. Under the condition that
sodium ascorbate reduces cupric into cuprous as a catalyst, a 1,3-
dipole cycloaddition reaction is carried out between NDA modified with an
azide group and
DNA modified with an alkynyl group, so that
DNA modified with
cane sugar invertase is fixed on magnetic beads; The magnetic beads are separated from a solution, the cleaned magnetic beads are dissolved into a
cane sugar solution to carry out
enzymatic hydrolysis, and then a reaction solution after
enzymatic hydrolysis is measured by adopting a blood
glucose meter, so that
copper ion concentration can be detected. The portable
copper ion concentration detection method provided by the invention takes the blood
glucose meter as a platform and can portably and quickly detect the copper ion concentration on site; and the portable copper ion concentration detection method can be applied to copper ion detection in a
human body serum sample, and the portable copper ion concentration detection method has the advantages of easy operation, low cost, high sensitivity and good specificity.