Method for detecting melamine and specific enzyme-linked immunologic reagent kit
An enzyme-linked immunosorbent reagent and melamine technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitable for on-site monitoring sample screening, high skill requirements for inspectors, and complicated equipment and equipment, so as to shorten the detection time Time-saving, easy-to-use, high-sensitivity effects
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Embodiment 1
[0037] Example 1. Preparation and use of a kit using a hapten-carrier protein conjugate as a coating source and an enzyme-labeled secondary antibody as an enzyme marker
[0038] 1. The detection principle of the kit with the melamine hapten and carrier protein conjugate as the coating source and the enzyme-labeled secondary antibody as the enzyme marker is as follows:
[0039] When the coating on the microwell strip of the microtiter plate was originally a conjugated melamine hapten and carrier protein, after adding the standard solution or sample solution to the microwell of the microtiter plate, the melamine-specific antibody was added, and the residue in the sample The melamine on the ELISA plate competes with the melamine-coupled antigen for the melamine-specific antibody, and then the enzyme-labeled anti-antibody is added for amplification, and the color is developed with a chromogenic solution. The absorbance value of the sample is negatively correlated with the content o...
Embodiment 2
[0141] Embodiment 2, the kit that is used to detect melamine can also have following several kinds:
[0142] 1. The original coating is a specific antibody, and the enzyme marker is an enzyme-labeled hapten kit
[0143] (1) The working principle of this kit is:
[0144]When the melamine-specific antibody described in Example 1 is pre-coated on the microwell strip, after adding the sample solution or standard solution, add the enzyme-labeled hapten solution. The melamine or melamine standard in the sample competes with the enzyme-labeled antigen for the melamine-specific antibody coated on the microtiter plate, and the color is developed with a chromogenic solution. The absorbance value of the sample is negatively correlated with the content of melamine in the sample, and compared with the standard curve The content of melamine in the sample can be obtained. At the same time, the concentration range of melamine in the sample can be roughly judged by comparing the color of the...
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