99 results about "Erythromycin thiocyanate" patented technology

The present invention discloses a method for detecting the erythromycin compounds and an enzyme linked immunoreaction reagent kit thereof which is specialized for the method. The enzyme linked immunoreaction reagent kit comprises an erythromycin specific antibody, a coatingen and an enzyme label. The erythromycin specific antibody is a monoclone antibody or polyclonal antibody of the erythromycin. The erythromycin compound is at least one in erythromycin, erythromycin thiocyanate and erythromycin ethylsuccinate. The erythromycin is erythromycin A. The enzyme linked immunoreaction reagent kit of the invention has the advantages of simple structure, convenient use, low cost, convenient carriage, high efficiency of the detecting method, accuracy and easiness. The on-spot monitoring can be executed. Furthermore the enzyme linked immunoreaction reagent kit is suitable for the qualitative and quantitative sieving of considerable samples, and can exert an important function in the detection to the erythromycin.

The invention provides a method for preparing erythromycin thiocyanate. The method comprises the following steps of: removing impurities from erythromycin fermentation liquor, and treating by using alkaline liquor; and extracting by using butyl acetate, collecting an organic phase, adding a sodium thiocyanate solution into the organic phase, controlling the pH value to be 4.0 to 6.0, stirring, crystallizing and drying to obtain erythromycin thiocyanate powder. According to the method, the erythromycin fermentation liquor is used as a raw material, and the refined erythromycin thiocyanate is obtained by impurity removal and a mode of combining extraction and the salifying of sodium thiocyanate. The method is easy to operate and low in cost, the requirement of mass production can be met, and a product is high in yield and purity.

The invention discloses a preparation method of erythromycin thiocyanate; the preparation method comprises the following steps of: (1) washing erythromycin thiocyanate crude product by 40-60 DEG C purified water, dissolving into 38-48 DEG C acetone, wherein the volume of acetone is 1-4 times of that of erythromycin thiocyanate crude product, adjusting pH (potential of hydrogen) to be 9.0-10.5, then washing by saturated NaCl, separating phases, throwing water phase away, so as to obtain acetone solution containing erythrocin alkali; and (2) adding thiocyanate into the erythrocin alkali acetone solution obtained in the step (1), acidifying, crystallizing, filtering, washing by purified water and drying, finally the erythromycin thiocyanate product is obtained. The erythromycin thiocyanate prepared by the method is excellent in quality, high in yield, low in production cost, and excellent in industrial application prospect.

The invention relates to a treatment process and device of wastewater produced by erythromycin thiocyanate. The treatment process comprises the following steps of: evaporating high-concentrate wastewater produced by erythromycin thiocyanate by an evaporator in cycle, mixing evaporated low-concentrate water with low-concentrate wastewater produced by the erythromycin thiocyanate, then carrying out hydrolytic acidification, adjusting the pH value, carrying out anaerobic microbial decomposition, precipitating the decomposed wastewater after treatment by active sludge, carrying out ozone oxidization treatment on a clear liquid, and precipitating and discharging after treatment by the active sludge. The wastewater disclosed by the invention adopts the biological treatment process of combining 'four-effect evaporator, hydrolytic acidification, two-stage contact oxidation process and advanced treatment (ozone-Fenton reaction)'. Thus, the treatment device is low in energy consumption, small in investment, high in efficiency, and stable and reliable in treatment effect.

The invention provides a method for refining erythromycin thiocyanate, which comprises the following steps: (1) fetching erythromycin thiocyanate crude product, dissolving in a solvent, treating with alkali, removing insoluble substances, adding thiocyanate, adding acid for adjusting pH to 5.5-8.3; (2) precipitating solid, washing, drying and obtaining the erythromycin thiocyanate finished product; wherein, the solvent in the step (1) is methanol, ethanol or isopropanol; Or, the solvent is a mixed solvent containing more than two ingredients among methanol, ethanol, isopropanol, water, acetone, dichloromethane, ethyl acetate and butyl acetate; in the mixed solvent, the contents of the methanol, ethanol or isopropanol are above 30% w / w. The invention also provides a preparation method of erythromycin thiocyanate. The invention overcomes the technology prejudice that the mixing and dissolving of solvent and water can not reduce the erythromycin impurity, and not only the purity and the yield of the erythromycin thiocyanate finished product are not reduced, but also the solvent cost is substantially saved and the technology step is simplified.

The invention relates to the technical field of crystallization engineering, in particular to a preparation method of erythromycin thiocyanate. The method comprises the following steps that a solventis added into crude erythromycin thiocyanate, a sodium hydroxide solution is used for regulating the pH value, and a mixed solution is obtained; sodium chloride solids are added into the mixed solution, standing is performed after stirring is performed, and phase splitting is performed to a supernate I; then, a saturated sodium chloride solution continues to be added into the supernate I, standingis performed after stirring is performed, phase splitting is performed, and a supernate II is obtained; a sodium thiocyanate solution is added into the supernate II, then, glacial acetic acid is added to regulate pH, reduced pressure distillation is performed, cooling is performed to reach 15-30 DEG C, and centrifuging is performed to obtain a wet erythromycin thiocyanate product; the wet erythromycin thiocyanate product is smashed and dried to obtain erythromycin thiocyanate. The preparation method of the erythromycin thiocyanate has the advantages that the technology is simple, control is easy, little wastewater is produced, the yield of the prepared erythromycin thiocyanate is high, and the purity of the prepared erythromycin thiocyanate is high.

The invention provides a refining method of erythromycin thiocyanate. The refining method of the erythromycin thiocyanate mainly comprises the following steps of: (1), preliminarily discoloring erythrocin filtrate by a flocculating agent to remove impurities, extracting the purified solution by a special-purpose erythromycin solvent, and further discoloring the extracting solution by active carbon to remove impurities; and (2), inverting the treated erythrocin extracting solution to a purifying water phase by phase inversion, extracting the thiocyanate in the water phase to a solvent mixture of n-butyl acetate and cyclohexane, thereby obtaining an erythrocin crystallization solution by utilizing a two-step crystallization method, and separating and drying to obtain an erythromycin thiocyanate finished product. The content of erythrocin A in the finished product is more than 83%. In order to solve the defect that the content of erythrocin A in the erythromycin thiocyanate finished product, which is prepared by extracting the erythrocin from erythrocin filtrate by utilizing special-purpose solvent, crystallizing the extracting solvent and finally separating and drying in a conventional production process, is low, the refining method of the erythromycin thiocyanate can be used for improving the content of the erythrocin A in the erythromycin thiocyanate finished product by discoloring the filtrate and the extracting solution to remove impurities and adopting a two-step crystallizing process after phase inversion.

The invention discloses a preparation method of erythromycin 6,9 imino ether. The method comprises the following steps: 1) with lower alcohols as reaction solvents, erythromycin thiocyanate reacts with hydroxylamine to generate thiocyanate salts of erythromycin; 2) water and water-immiscible low-polar organic solvents are added dropwise into the reaction mixture when the content of erythromycin detected with HPLC is less than 0.5%, lye is added into the mixture, after the mixture is fully mixed, to achieve a pH between 9 and 12, the mixture is allowed to stand to stratify, and a water layer is removed; 3) mesyl chloride or toluene sulfochloride is added into an organic layer for reactions of the Beckman arrangement to generate erythromycin 6,9 imino ether. The method provided by the invention simplifies operations by removing extraction of erythromycin oxime and salts of erythromycin oxime, and thus the conversion rate of erythromycin is improved and the cost of production equipment and energy is reduced. By means of the technology provided by the invention, the weight yield and the HPLC purity of erythromycin 6,9 imino ether synthesized by erythromycin thiocyanate can achieve more than 72% and more than 92% respectively.

The compound viral disease preventing and treating medicine preparation for silk worm consists of erythromycin thiocyanate as main medicine component and chlorine dioxide powder as auxiliary medicine component in the weight ratio of 1 to 3-10, preferably 1 to 4-6 and packed separately. When the medicine preparation is used, chlorine dioxide powder is first dissolved, erythromycin thiocyanate is then added for mixing homogeneously, water in 500 times is added and the medicine liquid is sprayed to mulberry leaf fed to silk worm to prevent and treat viral disease.

The invention belongs to the technical field of medicine intermediate synthesis and discloses an erythromycin A oxime clean synthetic method and specially-combined reaction equipment for the same. The method includes: using polar solvent to dissolve erythromycin thiocyanate, inorganic acid salt of hydroxylamine and water-soluble alkali, adding dehydrating agent, controlling certain outlet pressure through the specially-combined reaction equipment, constantly boiling for dehydration, completely performing oximation of erythromycin A after a certain time, cooling to enable complete precipitation of oximate, filtering, alkalizing the oximate conventionally, continuing oximation synthesis of oximate mother liquor after a certain quantity of raw materials are supplemented, and cycling so to enable the erythromycin thiocyanate and auxiliary materials to be fully used to improve overall yield. The specially-combined reaction equipment mainly comprises a water bath 1, a reaction vessel 2, a dephlegmator 3, a separator 4, a condenser 5, a stirring motor 6, a buffering bottle 7, a vacuum meter 8, a regulating valve 9, a vacuum pump 10 and a thermometer 11.

The present invention relates to an erythromycin thiocyanate emulsion, wherein erythromycin thiocyanate is weighed, is mixed with a dispersing agent in water, is blended with an auxiliary solvent, is subjected to thickening through a carrageenan, and then is subjected to volume metering to prepare the finished product. According to the present invention, with detection, the emulsions with different contents can be completely and uniformly mixed with water, the solubility of the emulsion is far higher than the monomer erythromycin thiocyanate, the character is stable and no precipitation is generated after long-term storage, and the agents with a plurality of concentrations can be prepared according to requirements of different diseases, such that the use is convenient.