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Method for preparing erythromycin thiocyanate

A technology of erythromycin thiocyanate and erythromycin, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low yield, high production cost, complicated operation, etc., and achieve product High yield and purity, suitable for mass production, and simple operation

Active Publication Date: 2012-09-12
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a simple and efficient method for preparing thiocyanate for the low purity of erythromycin thiocyanate product, impure color, high production cost, low yield and complicated operation in the prior art. A new approach to erythromycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. If not otherwise specified, the percentage sign "%" involved in the examples refers to the mass percentage; but the percentage of the solution, unless otherwise specified, refers to the grams of solute contained in 100ml of the solution. The preparation of embodiment 1 erythromycin thiocyanate

[0017] Preparation of erythromycin fermented liquid: using Streptomyces ruber (Streptomyces ruber) as fermentation strain, the preparation process is:

[0018] The seed tank culture medium is 6.0% of dextrin, 3.0% of soybean cake powder, 0.3% of ammonium sulfate, 1.0% of sodium chloride, 1.0% of calcium carbonate, and 2.0% of soybean oil, prepared with water. After the culture medium is sterilized, when the temperature of the tank drops to 34°C, insert the strain of Streptomyces rubrum into the seed tank by the differential pressure method. The...

Embodiment 2

[0025] The preparation of embodiment 2 erythromycin thiocyanate

[0026] The preparation method of erythromycin fermentation broth is the same as that described in Example 1.

[0027] 1) Take 170L of the above-mentioned erythromycin fermentation broth, the erythromycin content is 624.1g, heat it to 40°C, and filter it through a 0.01μm ceramic membrane. During the ceramic membrane filtration period, you need to add 425L water for dialysis. Concentrate to obtain 170L of membrane concentrate, add 1.47L of 20% sodium hydroxide solution to adjust the pH to 8.5.

[0028] 2) The alkaline membrane concentrate was passed through the treated resin column at a flow rate of 2BV / h to absorb impurities, and 171.47L of effluent was collected.

[0029] 3) Adjust the pH of the effluent to 10.3 with 20% sodium hydroxide solution, enter the extraction tower at a flow rate of 2.83ml / rev, and simultaneously feed butyl acetate into the extraction tower at a flow rate of 2.83ml / rev, stir, and colle...

Embodiment 3

[0032] The preparation of embodiment 3 erythromycin thiocyanate

[0033] The preparation method of erythromycin fermentation broth is the same as that described in Example 1.

[0034] 1) Take 55.7L of the above-mentioned erythromycin fermentation broth with a content of 223.5g of erythromycin, heat it to 45°C, and filter it through a 0.01μm ceramic membrane. The filter membrane was concentrated to obtain 55.7 L of membrane concentrate, and 250 ml of 20% sodium hydroxide solution was added to adjust the pH to 8.5.

[0035] 2) Pass the concentrated alkaline membrane solution through the treated resin column at a flow rate of 2BV / h to absorb impurities, and collect 55.95L of effluent.

[0036] 3) Adjust the pH of the effluent to 10.2 with 20% sodium hydroxide solution, enter the extraction tower at a flow rate of 2.83ml / rev, and simultaneously feed butyl acetate into the extraction tower at a flow rate of 2.83ml / rev, stir, and collect 4.6L of the upper layer Butyl acetate phase...

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Abstract

The invention provides a method for preparing erythromycin thiocyanate. The method comprises the following steps of: removing impurities from erythromycin fermentation liquor, and treating by using alkaline liquor; and extracting by using butyl acetate, collecting an organic phase, adding a sodium thiocyanate solution into the organic phase, controlling the pH value to be 4.0 to 6.0, stirring, crystallizing and drying to obtain erythromycin thiocyanate powder. According to the method, the erythromycin fermentation liquor is used as a raw material, and the refined erythromycin thiocyanate is obtained by impurity removal and a mode of combining extraction and the salifying of sodium thiocyanate. The method is easy to operate and low in cost, the requirement of mass production can be met, and a product is high in yield and purity.

Description

technical field [0001] The invention relates to the field of chemical industry, in particular to a method for preparing erythromycin thiocyanate from erythromycin fermentation liquid. Background technique [0002] Erythromycin is an alkaline glycoside synthesized from erythromycin lactone, erythromycin deoxysugaramine and erythromycin branch sugar, and can be divided into erythromycin A, erythromycin B, erythromycin C, erythromycin D and Erythromycin E. It is a white or off-white crystalline powder, easily soluble in alcohols and acetone, the solubility in water is 2mg / ml (25°C), and decreases with the increase of temperature, and the solubility is the smallest at 55°C. [0003] An organic base composed of it, in which amino acid molecules form a cyclic polypeptide structure, and fatty acids are located at the end of the polypeptide structure. According to the different types of terminal fatty acids, it is divided into enramycin A and enramycin B, and enramycin is a mixtur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06
Inventor 李荣杰
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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