84 results about "Pig liver" patented technology

The invention relates to the fields of analytical chemistry and food safety, in particular to a method for detecting the residual quantity of multiple alkaline drugs in animal derived food. Based on the vortex mixed extracting of acetonitrile, isopropanol and citric acid buffer solutions, the purification of a hydrophilic polystyrene-divinylbenzene solid phase extraction column and a cation exchange solid phase extraction column and the liquid phase chromatography-mass spectra determination, the method can detect the residual quantity of multiple alkaline drugs in pork, pork liver, eggs, shrimps and milk, such as beta-receptor agonists,sulfonamides, benzodiazepines, nitroimidazoles, benzimidazoles and triphenylmethanes. The method has the advantages of simple operation, fast and accurate detection and high efficiency.

The invention provides an immune colloidal gold test paper card for testing quinolones, comprising a reaction film, a sample pad, a conjugate release pad, a water absorbing pad, and a back liner, wherein, the reaction film comprises a test zone coated with quinolones-carrier protein conjugate and a quality control zone coated with sheep-anti-mouse IgG; the conjugate release pad coats quinolones monoclonal antibody-colloidal gold marker. The invention also provides the method using the test paper card to test quinolones, which comprises the following steps: firstly, pre-treating sample; secondly, testing the sample with the test paper card; thirdly, analyzing test result. The invention can be used to test residual quantity of quinolones in animal derived food such as chicken, chicken liver, pork, pig liver, urine, and honey, and has the advantages of simple operation, high sensitivity, fast test, low cost, on-site supervision, and satisfaction of test requirement of a large quantity of samples.

The present invention provides a colloid immunity test paper card for testing medicine sulfaquinoxaline dioxin, and includes a reaction membrane, a sample pad, a combination releasing pad, a drinking pad, and a back pad. The present invention is characterized in that a testing area with a coupling object of the coating sulfaquinoxaline dioxin medicine-carrier albumen and a quality control area ofthe coating goat-anti-mouse lgG are arranged on the reaction membrane. The combination releasing pad coats the sulfaquinoxaline dioxin medicine monoclonal antibodies- colloid metal marker. The present invention also provides a method to use the above medicine sulfaquinoxaline dioxin, and the method includes the following steps: pre-processing the sample, using the test paper card to conduct testing, and analyzing the test result. The test paper card provided by the present invention can be used to test the medicine residue of the sulfaquinoxaline dioxin in the Animal-derived food such as the chicken, the chicken liver, the pork, pig liver, the urine, and the honey. The present invention has simple operation, low cost, high sensitivity, and quick test speed; is applicable for large-scale popularization and mass sample screening; and can be controlled on-spot.

The invention relates to the field of food safety detection, and in particular discloses a detection method for a kanamycin A enzyme-linked aptamer and an application of the kanamycin A enzyme-linked aptamer. By a streptavidin-biotin-aptamer mode, kanamycin A can be detected; furthermore, a detection condition is optimized; and finally a condition with the lowest detection limit is obtained and the condition is high in sensitivity and specificity. According to the method, the kanamycin A in milk, pork, chicken, pork liver and honey can be detected according to the specificity; and the method has a wide application prospect.

Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line and assessed for their potential application in artificial liver devices. The study included assessments of growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production. The PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles. PICM-19H cells display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. Ultrastructural analysis of the PICM-19B monolayers show that the roughly cuboidal cells display basal-apical polarization and are joined by tight junction-like complexes. Other ultrastructure features are similar to those of PICM-19H cells except that they possess numerous cell bodies resembling mucus vacuoles. The PICM-19B cells possess relatively high levels of GGT activity, but retain some inducible CYP450 activity, and some ammonia clearance and urea synthesis ability. These data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. In vitro models of the liver are needed to replace animal models for the rapid assessment of drug biotransformation and toxicity. A unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. These cells have many activities associated with xenobiotic phase I and phase II metabolism lacking in other liver cell lines. The PICM-19H cell line was also compared to the tumor-derived human HepG2 C3A cell line and to primary cultures of adult porcine hepatocytes. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing and further support their use in artificial liver device technology.

The invention discloses a separation method and application of hepatic cells peripheral to pig portal veins or hepatic cells peripheral to hepatic veins. By means of the method, a great number of hepatic cells peripheral to the portal veins or hepatic cells peripheral to the hepatic veins which are high in activity can be separated out of a pig liver, the obtained cells can maintain good morphological characteristics, hepatocellular functions and cell multiplication activity of primary hepatic cells, and a basis is provided for separation and identification of the hepatic cells peripheral to the portal veins or the hepatic cells peripheral to the hepatic veins, in vitro study on cell metabolism differences of the hepatic cells peripheral to the portal veins or the hepatic cells peripheral toward the hepatic veins and deeper exploration on liver nutrient substance metabolism.

The invention discloses a screening method of Anhui local pig breed meat quality trait candidate genes. The screening method comprises following steps: (1) selection of reference animal models; (2) sample collection, wherein after pig slaughter, longissimus dorsi, heart, and liver tissues are collected; (3) conventional meat quality index determination, wherein meat color, pH value, and tenderness of longissimus dorsi are determined; (4) determination of longissimus dorsi IMP content via chromatography; (5) determination of longissimus dorsi IMF content via Soxhlet extraction; and (6) determination of expression level of key genes in pig liver, heart, and longissimus dorsi in IMP and IMF forming processes via RT-PCR, and data statistic analysis. The screening method is simple in operation, low in cost, and ideal in detection results.

The invention provides a colloidal gold immunologic test paper card for detecting enrofloxacin drugs, comprising a reaction film, a sample pad, a conjugate releasing pad, a water absorbing pad and a back lining, wherein the reaction film is provided with a test zone coated with the enrofloxacin drugs, namely carrier protein conjugates and a quality control zone coated with sheep-anti-mouse IgG; and the conjugate releasing pad is coated with a monoclonal antibody of the enrofloxacin drugs, namely a colloidal gold label. The invention also provides a method for applying the test paper card to detect the enrofloxacin drugs, including the following steps of sample preliminary treatment, detection by using the test paper card and detected result analysis. The test paper card can be used for detecting the residual quantity of the enrofloxacin drugs in animal foods, such as pork, pork liver, chicken, chicken liver, serum, fishes and shrimps, has the advantages of simple operation, high sensitivity, rapid detection speed and low cost, and can monitor on site and satisfy the requirement of screening a plurality of samples.

The invention discloses pig blood and liver blood-replenishing sauce which belongs to the technical field of foods and is mainly made from pig blood, pig liver, pepper, aniseed, ginger, dried red pepper, salt, monosodium glutamate, dried salted and fermented soya paste, sesame, peanut, garlic, peanut oil and sugar as raw materials according to the following steps of (1, slicing pig blood, blanching with boiled water and drying, cleaning and cooking the pig liver for later use; 2, adding the pepper, the aniseed, the ginger, the dried red pepper, the dried salted and fermented soya paste, the sesame, the peanut and the garlic in a product obtained in the step 1 for mixing, then grinding by using a meat grinder into a mud material for later use; and 3, boiling the peanut oil, pouring the mud material in the step 2 into a pot, adding a proper amount of pure water, repeatedly drying, stewing with slow fire for 3-5min, stopping fire and adding the salt, the sugar and the monosodium glutamate, uniformly stirring, and loading in a bottle for making the pig blood and liver blood-replenishing sauce.

The present invention relates to a preparation technology of pig liver peptide powder. The preparation technology comprises the following steps: (1) pig liver homogenizing, lipase enzymatic degreasing, protease hydrolyzing and ultrafiltering are conducted to obtain liver peptide liquid, and the liver peptide liquid is spray-dried to obtain a finished product. The enzymatic hydrolysis method is combined with ultrafiltration membrane separation technology to extract and purify small molecular liver peptides. The preparation technology is simple in processing processes, short in production cycle,high in production efficiency, low in cost, and suitable for large-scale industrial production. The obtained pig liver peptide powder is high in quality and has a crude protein content of 80% or more, and the small molecular liver peptides (with a relative molecular weight between 180 and 1,000 Da) account for 70% or more of the protein.

The invention discloses a method of detecting a content of phenylethanolamine A and a detection kit. According to the method, immunogens and coating antigens are prepared by synthesizing hapten derivatives of the phenylethanolamine A and crosslinking the derivatives with carrier proteins. Four kinds of polyclonal antibodies of rabbit anti-phenylethanolamine A through immunizing animals are obtained and all the four antibodies can be used for immunoassay of the phenylethanolamine A. The invention further provides an ELISA detection kit containing the antigens and / or antibodies. The ELISA method built by the invention is high in sensitivity and strong in specificity. Four kinds of samples: pig kidney, pig liver, pork and pig forage, are added with different amounts of the phenylethanolamine A for an addition and reclamation test, and the test shows that the detecting method provided by the invention is accurate, and has relatively good correlation with an LC-MS-MS method.

The invention relates to the technical field of separation and extraction of bioactive substances, in particular relates to a clean production technique for the separation and the extraction of catalase, and provides an environment-friendly technique for the separation and the extraction of catalase. The technique can realize zero-discharge of waste water and waste residue during the process of processing and producing catalase and radically solve the environment pollution problem in the prior technique of processing and producing catalase without influencing the yield and quality of catalase. The technique is suitable for the processing and the production of catalase by using the raw material of the liver of various animals such as pig, cattle, ship, chicken, duck, goose, fish, etc after autolysis treatment, and is also suitable for the processing and the production of catalase by using the catalase-containing fermentation liquid obtained through the fermentation and the cultivation of microbe. According to the invention, taking pig liver as an example, 2 kg of catalase liquid (50,000 U / ml) can be prepared from 1 kg of pig liver, the water consumption is reduced to lower than 2.2 kg, and 230 g of the solid substances with protein content being higher than 60% can be obtained at the same time.

The invention discloses a large-scale culture method of immortalized pork liver cells, which comprises: the immortalized pork liver cells collected are inoculated in a spinner bottle, rotating culture is carried out in a three-layer culture system, sodium alginate solution with a concentration of 1.5 percent to 2.0 percent is used for adjusting the concentration of the immortalized pork liver cells to 1 to 2.5 mulpied by 10<6> / mL; the immortalized pork liver cells obtained through readjustment of the concentration is prepared into immortalized pork liver cell micro-capsule by one-step method with alginic acid-chitosan; and the invention further discloses a microencapsulized immortalized pork liver cell. The microencapsulized immortalized pork liver cell can be used as liver cell source for the transplantation of biotype artificial liver or liver cell. The HCMV gBn1 antibody prepared has the advantages of strong specificity and high sensitivity.

The invention provides an efficient gene knockout vector based on a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeat / Cas9) system. The efficient gene knockout vector comprises atleast two DNA (deoxyribonucleic acid) fragments which are serially connected, target at different genes or different loci of genes and are designed according to sgRNA (small guide ribonucleic acid) target loci. Simultaneous knockout of double genes or multiple genes can be efficiently, rapidly and conveniently achieved, and meanwhile, damage to transfection cells can be reduced. The invention further provides a method for knocking out a pig liver enriched gene 1, and pig fibroblast of which diallele genes pLeg1a and pLeg1b are simultaneously knocked out.

The invention discloses a temperature-based real-time evaluation method and a temperature-based real-time evaluation device for the Young's modulus of a microwave ablation tissue. The method can be used to estimate the hardness parameter (Young's modulus, E) of the tissue in real time according to an obtained temperature (T) of the tissue in a microwave ablation process. According to the method, firstly, a large amount of synchronous change data of T and E in the microwave ablation process is acquired by building an in-vitro pig liver microwave ablation temperature and Young's modulus real-time synchronous acquisition system; and secondly, a plurality of groups of E-T relation equations are established by data fitting to acquire a final E-T relation model. The method establishes correlation between T and E, and can calculate the hardness parameter of the tissue by measuring the temperature of the tissue. The method has great significance for evaluating the real-time curative effect ofmicrowave ablation of tumors, and has important values for establishing a multi-mode microwave ablation curative effect evaluation system.

The invention belongs to the technical field of separation and extraction of bioactive substances, and in particular relates to a technique for preparing catalase and liver peptide by using raw material of animal liver. The technique is suitable for processing and production of catalase by using the raw material of animal liver after autolysis processing, and provides a novel technique of jointly producing catalase and liver peptide. The technique has the advantages that catalase and liver peptide can be obtained at the same time; no waste water and waste slag are discharged in the whole processing and production process; and environment pollution problem can be radically solved in the prior catalase processing and production technique. After providing catalase to animal liver, the remaining ezyme of liver peptide hydrolysates the product, so as to be utilized as a novel natural drug raw material or a functional health food raw material and widely used in the fields of drugs, health-care products, food, etc. By adopting the invention for operation, taking pig liver as an example, 2 kg of catalase (50,000 U / ml) can be prepared from 1 kg of pig liver, at the same time, 200 g of liver peptide powder with protein content reaching 70% can be obtained, and the water consumption is reduced to lower than 2.2 kg.

A process for preparing the hydrogen peroxidase by extracting method includes immersing fresh pig liver or ox liver in water for dissolving the hydrogen peroxidase contained in them in the water, adding penicillin, centrifugal separation, adding chitin solution to flocculate other proteins, frame filtering with diatomite, ultrafiltering, adding NaCl for deposition, adding formaldehyde solution for sterilizing and clarifying. The product can be used to decompose the residual H2O2 in dyeing fabric. Its advantages are high extracting efficiency, less loss of enzyme activity and no environmental pollution.

The invention discloses a method for preparing a seriously contaminated antique silk substitution sample. The method comprises the following steps of: A) putting commercially available habotai and a rusty iron product together in a humid environment for 10-15 days so that the commercially available habotai is stained with rust; B) soaking the commercially available habotai stained with rust in fresh pig blood for 12 h and then putting in a mixture of pig meat and pig liver according to a weight ratio of 1: (20-50), and standing for 5-7 days at a cool airtight place, wherein the mixture contains pig fat, pork lean, pig liver and water in a ratio of 2: 1: 1: 4; C) taking out of the commercially available habotai, scraping carrion attached to the surface of the habotai, performing aging treatment on the contaminated sample in a double aging way of ultraviolet rays and ozone, irradiating by means of an ultraviolet treatment box at a radiation intensity of 4000-4600 mu w / cm<2>, and simultaneously supplying ozone into the treatment box for 30 min every hour, wherein the ozone output quantity is 400 mg / h; and turning over the treated commercially available habotai every hour and treatingthe habotai for 50-80 hours. Compared with the prior art, the method is capable of simulating a cultural relic authentically, simple in operation and low in cost.

The invention discloses a microwave ablation tissue Young modulus real-time evaluation method based on a reduced scattering coefficient. The hardness (Young modulus, E) of a tissue is estimated in real time according to the obtained reduced scattering coefficient (mu's) of the tissue in the microwave ablation process. The method comprises the following steps: first, establishing an in-vitro pork liver microwave ablation reduced scattering coefficient and Young modulus real-time synchronous acquisition system to obtain a large amount of mu's and E synchronous change data in the microwave ablation process; and then, establishing a plurality of groups of E-mu's relation equations through data fitting, and obtaining a final E-mu's relation model by taking a determination coefficient (goodnessof fit) of each group of equations as a weight. According to the method, the correlation between mu's and E is established, and the hardness parameter of the tissue can be calculated by measuring thereduced scattering coefficient of the tissue. The method has great significance in evaluating the real-time curative effect of tumor microwave ablation, and has important value in establishing a multi-mode tumor microwave ablation curative effect evaluation system.

The invention relates to a microorganism feed additive capable of protecting pig livers and a preparation method of the microorganism feed additive. All strains adopted by the microorganism feed additive are screened out from intestinal tracts of animals, and a strain compound culturing method is adopted, so that an antagonism mechanism of the strains is avoided, and fermented products are optimized; and in the whole production technology, beneficial microorganisms are furthest maximized, and a main fermented product namely glutathione is wholly recovered from intracellular and extracellular positions. According to the microorganism feed additive and the preparation method thereof disclosed by the invention, oxygen free radicals can be effectively eliminated, so that the stability of liver cell membranes is improved, the activity of liver enzymes is promoted, the absorption of iron can also be promoted, the integrity of red cell membranes can be maintained, the biosynthesis of DNA is maintained, the normal growth of cells is maintained, and cell immunity is maintained; as a micro-ecologic preparation, the microorganism feed additive can effectively promote the digestion and the absorption of the feed by the animals, the lean meat percentage is increased, toxin disoperation can be alleviated, the feed conversion rate is increased, and the production capacity of bred animals is improved.

The present invention relates to the field of biotechnology, and discloses an anti-beta receptor agonist cluster-specific monoclonal antibody hybridoma cell line and a monoclonal antibody secreted bythe cell line. According to the present invention, the hybridoma cell line is prepared from salbutamol artificial antigen, and the secreted monoclonal antibody can simultaneously recognize salbutamol,clenbuterol, terbutaline, brombuterol, cimbuterol and other beta-receptor agonists, and has cluster specificity; the immunoaffinity chromatography gel prepared from the monoclonal antibody and the immunoaffinity chromatography gels of other beta-receptor agonists such as ractopamine are mixed as the machine measurement pretreatment purification reagent, a variety of clenbuterol hydrochloride components in pork, pig liver and pig urine samples can be efficiently detected by combining the machine measurement pretreatment purification reagent, HPLC-FLD and LC-MS / MS, and the high accuracy and high sensitivity can be achieved.

The invention provides a process for producing polyaluminum ferric chloride by gasifier ash. The process comprises the steps of raw material selection, leaching, acid washing, solid-liquid separation and drying and crystallization. The raw materials are gasifier ash. In the acid washing step, the mass ratio of the filtering slag to the acid liquid is 31:77; pig liver pulp is added into a reaction kettle; the addition of the pig liver pulp is 1 / 10 to 1 / 6 of the mass of the filter slag; the reaction temperature is 75 to 80 DEG C; the reaction time is 30min; the drying temperature is 42 to 50 DEG C. The process for producing polyaluminum ferric chloride by gasifier ash has the advantage that the product yield is 96.5 to 99.5 percent.

A nonlinear correction method of cell DNA detection equipment comprises the following steps: setting an index model transformation adjusting parameter, a Bayer mode analysis adjusting parameter, and an image segmentation threshold compensation parameter; adjusting the microscope in cell DNA detection equipment, placing a standard pig liver correction slice, setting a scanning zone, then starting to scan; obtaining a scan image after scanning, obtaining quality control parameters through system calculation on the scan image, comparing the obtained quality control parameters with standard quality control parameters in the system, adjusting the index model transformation adjusting parameter and Bayer mode analysis adjusting parameter according to the deviation; and repeating the steps mentioned above until the deviation between the calculate quality control parameters and corresponding standard quality control parameters in the system falls in an allowable error range so as to complete the correction. The detection equipment is corrected and adjusted by adjusting several parameters of a nonlinear mode and adopting a limited iterative method, and the corrected equipment can be applied to normal work quickly.

The invention belongs to the technical field of separation and extraction of bioactivator, in particular relating to a process for extracting hepatocyte growth-promoting decoction from waste water and waste residues after extraction of catalase. The process takes animal liver as raw material to prepare catalase by homogenate, solution soaking, solid-liquid separation and primary membrane separation; waste residues merge with primary membrane penetrating liquid after being hydrolyzed by enzyme process and removed by filtering, and suffers from secondary membrane separation and cryoconcentration to obtain the hepatocyte growth-promoting decoction. The decoction can prepare medicine of solution of hepatocyte growth-promoting factors after being purified, or prepare functional food with function of protecting liver after being cold-dried. Taking pork liver for an example, 1kg pork liver can be processed to obtain 2L of liquid caralase with enzyme activity unit being 50000U / mL by operating according to the invention, 200mL of hepatocyte growth-promoting decoction is obtained simultaneously with index of stimulation being 2.3 and total amino acid being 62.91g / 100g.

The invention provides a pig frozen semen basic diluent. The basic diluent comprises glucose, citric acid, cholesterol, L-proline, Tris and double distilled water. A frozen pig semen cryopreservationliquid further comprises the following components in percentage by volume: 20% of fresh yolk, 1*10<6> IU / L of penicillin, 1*10<6> IU / L of streptomycin, 3%-4% of glycerinum and 1%-2% of Equex.STM. A pig fresh-keeping semen diluent further comprises isotonic skimmed milk. According to the invention, the survival rate of local pig fresh semen in the long-distance transportation process can be effectively maintained, and the damage of pig sperms in the freezing process is prevented or significantly reduced by adding pig liver-derived cholesterol, L-proline and an antifreeze agent Equex.STM; and through an effective thawing method, the operation is convenient, and the sperm motility and quality are improved.

The invention discloses an in-vitro construction method of a pig liver tissue organoid model. The method comprises the steps of shearing and cleaning the liver tissues of a newborn piglet, and addingdigestive juice for incubation and digestion to obtain organoid fine particles; mixing the organoid fine particles with matrix gel, and performing inoculation; and after the matrix gel is solidified,performing incubation for 4 days by using an induction culture medium, then performing culture by using an expansion culture medium, and replacing the expansion culture medium once every 3-4 days, soas to obtain the pig liver tissue organoid model. The construction method is simple, rapid in construction and high in operability; the organoid model is constructed directly through the organoid fineparticles of a newborn animal; the organoid model not only can truly reflect the internal environment in a living pig model, but also can also be used for studying liver growth and lipid metabolism in the living pig model at the molecular cell level; and sampling of living pigs is reduced.

The invention discloses a method for primary culture of pig hepatocytes, which is characterized in that it includes sequentially obtaining the liver, briquetting liver tissue, culturing liver cells, and identifying primary cells, wherein the briquetting of liver tissue is obtained by The liver was placed in a sterilized petri dish, cut into pieces, placed in a 30-60 mesh sieve and rolled, and the filtered liver tissue pieces were collected in a sterilized container. The present application adopts the method of culturing tissue blocks by rolling them with mesh sieves, and obtains suitable pig liver tissue blocks by rolling them with mesh sieves, the size of which is suitable for adherent growth, and the growth of liver cells is good.

The invention relates to a novel compound nutrition product with a liver injury repairing effect. According to the product, anthony pig liver peptide powder is selected to be used as the principle raw material, and various nutrients such as choline, inositol, zinc gluconate and methionine of the scientific proportioning, vitamin B, vitamin C and vitamin E are added. The liver injury repairing product is advanced in technology, high in biological activity, and capable of repairing liver injuries for various reasons, promoting hepatic cell regeneration, preventing fat from being accumulated in hepatic cells and enhancing the physiological functions of the liver.

The invention discloses a pig liver and spleen duct stem cell separation and three-dimensional organoid culture method. The method comprises two parts of dissociating pig liver and spleen tissue witha two-step method to obtain duct stem cells and carrying out three-dimensional culture on pig liver and spleen organoids. According to the three-dimensional organoid culture method based on pig liverand spleen duct stem cell separation, the technical problem of pig liver and spleen organoid establishment is solved, and the application of the organoid technology in animal nutrition research is promoted.

The invention discloses a preparation method for a superparamagnetic ferriferrous oxide nanoparticle, belonging to the field of ferriferrous oxide nanoparticles. The preparation method comprises the following steps: extracting free trivalent iron ions from a pork liver; then adding divalent iron and allowing nanometer ferriferrous oxide to the produced through alkaline adjustment; and then carrying out calcining and magnetic separation so as to obtain superparamagnetic ferriferrous oxide. The ferriferrous oxide nanoparticle prepared in the invention has water solubility, superparamagnetism, good dispersibility and uniform particle size distribution and be applied to the technical fields of biomedicine and biology.