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Preparation method for superparamagnetic ferriferrous oxide nanoparticle

A ferroferric oxide and superparamagnetic technology, applied in iron oxide/iron hydroxide, nanotechnology, nanotechnology, etc., can solve the problems of capillary blockage, poor water solubility, long synthesis cycle, etc., and achieve uniform particle size distribution , low cost and simple preparation process

Inactive Publication Date: 2016-08-10
TRUSYN CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: aiming at preparing nano-Fe 3 o 4 Particles are prone to agglomeration between particles during washing, filtering and drying, and their water solubility is poor, resulting in a long synthesis cycle, and they are used in biomedicine due to the problem that capillaries may be blocked due to excessive particle size, providing a superparamagnetic tetroxide The preparation method of ferric iron nanoparticles, the present invention extracts free ferric iron ions from pig liver, and then adds divalent iron to make it generate nanometer iron ferric oxide under the regulation of alkalinity, and finally undergoes calcination, magnetic The superparamagnetic ferric iron tetroxide can be obtained by separation, and the ferric ferric oxide obtained in the present invention has water solubility, superparamagnetism, good dispersion and uniform particle size distribution, and can be well applied in the fields of biomedicine and biotechnology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] Weigh 200g of fresh pork liver, mince the liver with a meat grinder, take 20g of minced pork liver into a 500mL conical flask, add deionized water according to the solid-liquid ratio of 1:6, stir and mix Then adjust the pH to 2.0 with a concentration of 1.5mol / L hydrochloric acid, add 0.3% pepsin-hydrochloric acid solution to the mixture after adjustment, and react with constant temperature shaking at 37℃ and 100r / min for 2h; after the end of the shaking reaction, use the concentration Adjust the pH to 6.0 with 1mol / L sodium bicarbonate solution. After adjustment, add 1% trypsin-bile salt solution of total mass. Stir and mix well. Put the dialysis bag containing 50mL of distilled water into the conical flask. The flask is placed in a shaker and shaken at 37°C and 100r / min for 3 hours. After the shaking, the dialysis bag is taken out; the mixed liquid in the conical flask is filtered to obtain the filtrate, and then the liquid in the dialysis bag and the filtrate are taken...

example 2

[0030] Weigh 250g of fresh pork liver, mince the liver with a meat grinder, take 25g of minced pork liver into a 500mL conical flask, add deionized water according to the solid-liquid ratio of 1:6, stir and mix Then use 1.5mol / L hydrochloric acid to adjust pH 2.3, add 0.4% total mass of pepsin-hydrochloric acid solution to the mixture after adjustment, and react with constant temperature shaking at 37℃ and 110r / min for 2.5h; after shaking reaction, use Adjust the pH to 6.3 with a sodium bicarbonate solution with a concentration of 1mol / L. After the adjustment, add 2% trypsin-bile salt solution of total mass, stir and mix well, put the dialysis bag containing 50mL of distilled water into the conical flask, Place the Erlenmeyer flask in a shaker and oscillate for 3.5h at 37°C and 110r / min. After shaking, take out the dialysis bag; filter the mixture in the Erlenmeyer flask to obtain the filtrate, and then take out the liquid and filtrate in the dialysis bag Mix by volume ratio 1:...

example 3

[0034] Weigh 300g fresh pork liver, mince the pork liver with a meat grinder, take 30g minced pork liver into a 500mL Erlenmeyer flask, add deionized water according to the solid-liquid ratio of 1:6, stir and mix After adjusting the pH 2.5 with a concentration of 1.5mol / L hydrochloric acid, add 0.5% pepsin-hydrochloric acid solution of total mass to the mixture after adjustment, and react with constant temperature shaking at 37℃ and 120r / min for 3h; after the shaking reaction, use the concentration Adjust the pH to 6.5 with 1mol / L sodium bicarbonate solution, add 3% total mass of trypsin-bile salt solution after adjustment, stir and mix well, put the dialysis bag containing 50mL of distilled water into the conical flask, put the cone The flask is placed in a shaker and shaken at 37°C and 120r / min for 4 hours. After the shaking, the dialysis bag is taken out; the mixed liquid in the conical flask is filtered to obtain the filtrate, and then the liquid in the dialysis bag and the ...

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Abstract

The invention discloses a preparation method for a superparamagnetic ferriferrous oxide nanoparticle, belonging to the field of ferriferrous oxide nanoparticles. The preparation method comprises the following steps: extracting free trivalent iron ions from a pork liver; then adding divalent iron and allowing nanometer ferriferrous oxide to the produced through alkaline adjustment; and then carrying out calcining and magnetic separation so as to obtain superparamagnetic ferriferrous oxide. The ferriferrous oxide nanoparticle prepared in the invention has water solubility, superparamagnetism, good dispersibility and uniform particle size distribution and be applied to the technical fields of biomedicine and biology.

Description

technical field [0001] The invention discloses a preparation method of superparamagnetic iron ferric oxide nanoparticles, which belongs to the field of iron ferric oxide nanometers. Background technique [0002] In recent years, superparamagnetic iron oxide nanoparticles have been widely used in the fields of biomedicine and biotechnology, such as gene-targeted drug delivery, cell separation, automated DNA extraction, hyperthermia, and magnetic resonance. The provided nano-magnetic beads must have the characteristics of biocompatibility, water solubility, superparamagnetism, etc., and also require a long time in blood vessels, that is, they can escape the phagocytosis of the reticuloendothelial system in the body. In addition, the particle size range of magnetic beads also has certain requirements: on the one hand, it needs to have a larger particle size to generate sufficient mechanical force; on the other hand, if the particle size is too large, it may block capillaries, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08B82Y30/00H01F1/11
CPCC01G49/08B82Y30/00C01P2004/32C01P2004/64C01P2006/42C01P2006/80H01F1/11
Inventor 吴蓉蓉王统军
Owner TRUSYN CHEM TECH
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