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Preparation technology of pig liver peptide powder

A preparation process, the technology of pig liver peptide powder, which is applied in the field of protein peptide preparation, can solve the problems that there are not many liver peptide powder technology methods and there are no patent reports, and achieve simple process flow, ensure stability and biological activity, and production efficiency high effect

Inactive Publication Date: 2019-07-02
陈石良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are not many in-depth research and development of animal liver in China, and there is no patent report on the process of using pig liver to prepare liver peptide powder.

Method used

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  • Preparation technology of pig liver peptide powder
  • Preparation technology of pig liver peptide powder

Examples

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Effect test

Embodiment 1

[0021] (1) Take 100kg of fresh pig liver that has passed the quarantine, remove the fascia, remove blood, wash, wash, add deionized water 3 times the weight of the pig liver, and homogenize with a homogenizer to obtain liver serum; ( 2) Heat the liver serum to 40°C, adjust the pH to 8.5, add 0.1% lipase to the total weight of the liver serum, stir for 5 minutes, stop stirring, keep warm for 60 minutes, then heat up to 95°C to inactivate the enzyme for 15 minutes , after cooling to room temperature, centrifuge at 4000rmp for 15 minutes, collect the precipitate, add deionized water 3 times the weight of the precipitate to the precipitate and stir evenly to obtain the defatted liver protein slurry; (3) raise the temperature of the defatted cerebroprotein slurry to 45°C, add 0.15% of the total weight of pig liver protease, adjust the pH value to 8.0, stir for 5 minutes, stop stirring, keep warm for 8 hours, heat up to 95°C to kill the enzyme for 15 minutes, cool to room temperature...

Embodiment 2

[0023] (1) Take 100kg of fresh pig liver that has passed the quarantine, remove the fascia, remove blood, wash, wash, add deionized water 3 times the weight of the pig liver, and homogenize with a homogenizer to obtain liver serum; ( 2) Heat the liver serum to 42°C, adjust the pH value to 8.8, add 0.08% lipase of the total weight of the liver serum, stir for 5 minutes, stop stirring, keep warm for 60 minutes, then heat up to 95°C to inactivate the enzyme for 15 minutes , after cooling to room temperature, centrifuge at 4000rmp for 15 minutes, collect the precipitate, add 4 times the weight of the precipitate to the deionized water and mix well to obtain the defatted liver protein slurry; (3) raise the temperature of the defatted liver protein slurry to 45°C, add 0.2% of the total weight of pig liver protease, adjust the pH value to 8.2, stir for 5 minutes, stop stirring, keep warm for 6 hours, heat up to 95 ° C to kill the enzyme for 15 minutes, cool to room temperature, and ob...

Embodiment 3

[0025] (1) Take 100kg of frozen fresh pork liver that has passed the quarantine, thaw the fascia, remove blood, wash, add deionized water twice the weight of the pig liver, and homogenize with a homogenizer to obtain liver serum; (2) Heat the liver serum to 45°C, adjust the pH value to 9.0, add 0.05% lipase to the total weight of the liver serum, stir for 5 minutes, stop stirring, keep warm for 60 minutes, heat up to 95°C to inactivate the enzyme for 15 minutes, cool After reaching room temperature, centrifuge at 4000rmp for 15 minutes to collect the precipitate, add deionized water 5 times the weight of the precipitate to the precipitate and stir evenly to obtain the defatted liver protein slurry; (3) raise the temperature of the defatted liver protein slurry to 50°C, add pig liver 0.3% of the total weight of protease, adjust the pH value to 8.5, stir for 5 minutes, stop stirring, keep warm for 4 hours, heat up to 95°C to kill the enzyme for 15 minutes, cool to room temperatur...

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Abstract

The present invention relates to a preparation technology of pig liver peptide powder. The preparation technology comprises the following steps: (1) pig liver homogenizing, lipase enzymatic degreasing, protease hydrolyzing and ultrafiltering are conducted to obtain liver peptide liquid, and the liver peptide liquid is spray-dried to obtain a finished product. The enzymatic hydrolysis method is combined with ultrafiltration membrane separation technology to extract and purify small molecular liver peptides. The preparation technology is simple in processing processes, short in production cycle,high in production efficiency, low in cost, and suitable for large-scale industrial production. The obtained pig liver peptide powder is high in quality and has a crude protein content of 80% or more, and the small molecular liver peptides (with a relative molecular weight between 180 and 1,000 Da) account for 70% or more of the protein.

Description

technical field [0001] The invention relates to a preparation method of protein peptide, in particular to a preparation process of pig liver peptide powder. Background technique [0002] Liver is one of the main by-products of pigs after slaughter, accounting for about 1.4% of pig body weight. Pork liver is extremely rich in nutrients. Every 100 grams of pork liver contains 21.3 grams of protein, twice that of pork, 4.5 grams of fat, 1.4 grams of carbohydrates, and contains a variety of vitamins and mineral elements. my country is a big pig producing country, with more than 500 million pigs slaughtered every year. According to the weight of each pig liver of 1.5 kg, the annual output of pig liver will reach 750,000 tons. Therefore, the pig liver raw materials in my country are not only very cheap, but also extremely rich in resources. However, due to various reasons, except that a small amount of pig liver is used for cooking or primary processing products, a considerable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 陈石良
Owner 陈石良
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