67 results about "Synthetic antigen" patented technology

The invention discloses an anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. According to the preparation, 10-70 parts by weight of porphyromonas gingivalis thalli and thalli fragments, and 30-90 parts by weight of fusobacterium nucleatum thalli and thalli fragments are compounded into an antigen to immune an egg laying hen. The invention also discloses the specific preparation method of the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation, and toothpaste containing the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. By the IgY toothpaste which is used for treating and preventing gingivitis and ozostomia has obvious effects in preventing and treating the gingivitis and the ozostomia.

The present invention relates to synthetic antigen-presenting matrices, their methods of making and their methods of use. One such matrix is cells that have been transfected to produce MHC antigen-presenting molecules and assisting molecules such as co-stimulatory molecules. The matrices can be used to activate CD8⁺ T-cells to produce cytokines and become cytotoxic.

This invention is in the field of recombinant Plasmodium falciparum polypeptides and relates to recombinant or synthetic antigen compositions which comprise p42 antigens, and more specifically to methods and compositions for the expression of Plasmodium falciparum polypeptides in transgenic plants.

The invention provides a method of detecting small molecule substances based on chemiluminescence immunology of golden-magnetic particles. The method mainly comprises the following steps: (1) coating: a step of using a golden-magnetic particle as carriers for immunoreaction and solid separation, using a small molecule substance corresponding to a small molecule substance to be detected to synthesize an antigen and coupling the antigen onto the surface of the golden-magnetic particle; (2) enclosing: a step of enclosing blank sites on the surface of the golden-magnetic particle which have not bound to the antigen by using blocking buffer , carrying out magnetic separation and discarding supernatant; (3) reaction with the substance to be detected: a step of adding a sample to be detected, an antibody against the small molecule substance to be detected and enzyme labeled secondary antibody capable of specifically binding to the antibody into the golden-magnetic particle which has bonded with the antigen synthesized from the small molecule substance so as to form a specific antigen-antibody-enzyme labeled secondary antibody compound; (4) cleaning and (5) detection. The detection method provided in the invention has the advantages of high sensitivity, good specificity, a wide linear range, high precision, good stability, no radioactive pollution, safe operation, simpleness and rapidity.

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.

The invention relates to a detection technology for detecting paralytic shellfish poisoning, in particular to a method for preparing an anti-paralytic shellfish poisoning monoclonal antibody by using protein coupling to synthesize an antigen, and relates to the use of monoclonal antibody and gonylin GTX2/3 antigen Combination, and the preparation method of the quick detection kit that analyzes the content of gonylin GTX2/3 in the biological sample. An enzyme-linked immunoassay kit for rapid detection of the main component of paralytic shellfish poisoning, gonyellin GTX2/3, including a 96-well microtiter plate with detachable strips, enzyme-labeled secondary antibody, and buffer salt wash containing Tween-20 solution, substrate solution and stop solution, using the prepared monoclonal antibody containing specific anti-gonitoxin GTX2/3, on the 96-well ELISA plate coated with the coated antigen of the synthetic protein conjugate. The kit of the invention has the advantages of simple pretreatment, easy operation, and is suitable for on-site, fast, and large-volume sample detection. The kit has low cost and low price, and the detection limit of the kit is less than 15ng/ml.

The invention discloses a comprehensive detection method for an allergen in a TCM injection, and relates to the field of immunology and science of TCM. According to the invention, the method is based on combined ELISA (enzyme linked immunosorbent assay) principles and fingerprint chromatogram variance analysis, uses an immunoreaction of embedding antigens, IgE antibodies or anti-IgE antibodies with solid carriers and respectively carries out labeled immunoreaction between TCM injection component antigens and labeled anti-IgE antibodies, the embedded IgE antibodies and labeled antigens, the embedded IgE antibodies and known synthetic antigens, the embedded IgE antibodies and enzyme labeled antibodies, the embedded IgE antibodies and IgE antibodies in a blood sample captured by the embedded IgE antibodies, the labeled antigens or antigens and labeled antibodies; the captured IgE antibodies are combined with TCM injection components, and fingerprint chromatogram variance analysis is carried out by using GC/MS and HPLC/MS; specific IgE and corresponding allergens in human and animal blood samples allergic to the TCM injection are determined based on comprehensive consideration of information of a plurality of aspects. The detection method for the TCM injection provided in the invention has the characteristics of high specificity, high sensitivity, high efficiency and easy and convenient operation, is applicable to simultaneous detection of a plurality of components and can effectively prevent omission.

The invention discloses a drug trace detection test strip, a preparation method thereof and a drug trace detection kit. The drug trace detection test strip comprises a test strip body, wherein the test strip body comprises a substrate, and a sample pad, a combination pad, a detection film and an absorption pad which are arranged on the substrate and are sequentially connected from one end to the other end of the substrate; the combination pad is provided with at least one time-resolved fluorescent microsphere-labeled specific monoclonal antibody of drugs; the detection film is provided with atleast one detection line corresponding to each drug; each detection line is coated with a synthetic antigen of the corresponding drug; and the six drugs are one or more of morphine, phenylalanine, ketamine, tetrahydrocannabinol, cannabis synthetic hormone and cocaine. The drug trace detection test strip has the advantages of high detection speed, low cost and simple operation.

Compositions and methods for the detection of Taenia solium and the diagnosis of T. solium infection are described. The nucleotide and amino acid sequences of the antigenic T. solium polypeptides gp50a, gp50b and gp50c are provided. The compositions contain synthetic antigenic polypeptides of larval origin prepared using the sequences described herein. Probes and primers for the detection or amplification of T. solium nucleic acid molecules are also described. The polypeptides can be administered to a human or animal to protect against T. solium infection. In addition, the polypeptides are useful as research tools for studying T. solium and as reagents in assays for the detection of T. solium antibodies in a biological sample. The methods are sensitive and specific assays that utilize the stable recombinant or synthetic antigenic polypeptides or nucleic acid molecules encoding the larval polypeptides.

The invention provides a method for intensifying functions of cytokine-induced killer cells. The method comprises the following steps: analyzing and obtaining an epitope through a bioinformatics analysis method, fusing the epitope and Ii-Kye into an epitope peptide, anchoring the epitope peptide on MHC-II molecules on the surfaces of dendritic cells, and infecting the dendritic cells with recombinant adenovirus rAd-RNAi-SOCS1; and further carrying out co-cultivation on the dendritic cells and cytokine-induced killer cells, and treating tumor-bearing mice with co-cultivated cells, so that the processed dendritic cells are capable of activating the cytokine-induced killer cells, and intensifying the significant killing functions of the cytokine-induced killer cells on related tumors. The invention provides a method for treating tumors employing activated cells; and the core technology of the method has reference significance on treatment of other viroses.

The invention discloses a flavanone-3-hydroxylase antigen epitope peptide, a fusion protein and a specific antibody of the flavanone-3-hydroxylase antigen epitope peptide, and a preparation method andapplication of the antibody. The amino acid sequence of the flavanone-3-hydroxylase antigen epitope peptide is as shown as SEQ ID NO:1. After being modified, the antigen epitope peptide is subjectedto solid-phase synthesis and is coupled with a carrier protein to obtain the fusion protein used as an artificially synthetic antigen, and the amino acid sequence is as shown as SEQ ID NO:2. The specific antibody of the invention is obtained by immunizing animals by the artificially synthetic antigen and performing separation and purification. The prepared polyclonal antibody has the advantages ofhigh affinity, high specificity, high sensitivity, operation simplicity and convenience, and can perform specific binding reaction with rice flavanone-3-hydroxylase; in addition, the preparation costis low; the yield is high; and an important tool is provided for the relevant physiological function study and identification of the rice flavanone 3-hydroxylase.

The invention relates to a latex microsphere immunodetection test strip, a kit and a method. The latex microsphere immunodetection test strip comprises a sample pad, a combination pad, a detection membrane, an absorption pad and a bottom plate, wherein the sample pad, the combination pad, the detection membrane and the absorption pad are sequentially connected from one end to the other end of the bottom plate; the combination pad is coated with a specific monoclonal antibody marked by latex microspheres, the specific monoclonal antibody marked by the latex microspheres can react with drugs, and the drugs comprise phencyclidine and oxycodone; a detection line and a quality control line formed by quality control protein are arranged on the detection film, and the detection line is coated with synthetic antigens of corresponding drugs. The latex microsphere immunodetection test strip can rapidly detect oxycodone and phencyclidine in hair, is high in sensitivity and good in specificity, does not need a detection instrument, and is convenient to operate.

The invention relates to a drug detection test strip and a drug detection kit. The drug detection test strip comprises a sample pad, a combination pad, a detection film, an absorption pad and a bottom plate, wherein the sample pad, the combination pad, the detection film and the absorption pad are sequentially connected on the bottom plate; the combination pad is provided with a specific monoclonal antibody marked by luminous microspheres of at least one drug, the detection film is provided with at least one detection line corresponding to the drug, the detection line is coated with a synthetic antigen corresponding to the drug, and the drug comprises at least one or a composition of hemp, an echinoka and cocaine. The drug detection test strip can detect three common drugs in hair at the same time, is convenient to take materials, stable to store, high in sensitivity and good in specificity, can effectively improve the detection efficiency of law enforcement officers, and meanwhile, reduces the detection cost.

Novel polypeptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.