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Anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY antibody, preparation method and toothpaste thereof

A technology of Porphyromonas and Fusobacterium nucleatum, applied in the direction of antibodies, antibacterial immunoglobulins, dentistry, etc., to achieve obvious effects

Active Publication Date: 2013-04-03
上海美加净日化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Technicians cannot know in advance that anti-Porphyromonas gingivalis and Fusobacterium nucleatum composite IgY are more effective than individual anti-Porphyromonas gingivalis IgY and individual anti-Porphyromonas more effective against Fusobacterium nucleatum IgY

Method used

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  • Anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY antibody, preparation method and toothpaste thereof
  • Anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY antibody, preparation method and toothpaste thereof
  • Anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY antibody, preparation method and toothpaste thereof

Examples

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Embodiment 1

[0036] Preparation of compound specific IgY preparation against Porphyromonas gingivalis and Fusobacterium nucleatum:

[0037]A. Porphyromonas gingivalis and Fusobacterium nucleatum culture steps

[0038] A.1 Bacterial species: Porphyromonas gingivalis and Fusobacterium nucleatum;

[0039] A.2 Porphyromonas gingivalis (P.g) ATCC33277 strain and Fusobacterium nucleatum (Fn) ATCC10953 strain were taken, and the Porphyromonas gingivalis and Fusobacterium nucleatum (Fn) ATCC10953 strains were separated and cultured on BHI-S medium, respectively. bacilli, and were identified and confirmed as Porphyromonas gingivalis and Fusobacterium nucleatum to obtain Porphyromonas gingivalis cells and cell fragments, Fusobacterium nucleatum cells and cell fragments;

[0040] B. Immunogen preparation steps:

[0041] B.1 Take 10 to 70 parts by weight of Porphyromonas gingivalis cells and cell fragments, and 30 to 90 parts by weight of Fusobacterium nucleatum cells and cell fragments to form a ...

Embodiment 2

[0052] Preparation of compound specific IgY preparation against Porphyromonas gingivalis and Fusobacterium nucleatum:

[0053] A. Porphyromonas gingivalis and Fusobacterium nucleatum culture steps

[0054] A.1 Bacterial species: Porphyromonas gingivalis and Fusobacterium nucleatum;

[0055] A.2 Porphyromonas gingivalis (P.g) ATCC33277 strain and Fusobacterium nucleatum (Fn) ATCC10953 strain were taken, and the Porphyromonas gingivalis and Fusobacterium nucleatum (Fn) ATCC10953 strains were separated and cultured on BHI-S medium, respectively. bacilli, and were identified and confirmed as Porphyromonas gingivalis and Fusobacterium nucleatum to obtain Porphyromonas gingivalis cells and cell fragments, Fusobacterium nucleatum cells and cell fragments;

[0056] B. Immunogen preparation steps:

[0057] B.1 Take 10-70 parts by weight of Porphyromonas gingivalis thalline and thalline fragments, and 30-90 parts by weight of Fusobacterium nucleatum thallus and thalline fragments to...

Embodiment 3

[0068] Separately take Porphyromonas gingivalis cells and cell fragments as bacterial liquid; prepare anti-Porphyromonas gingivalis specific IgY extracts separately. Refer to Example 1 for the rest of the preparation.

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Abstract

The invention discloses an anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. According to the preparation, 10-70 parts by weight of porphyromonas gingivalis thalli and thalli fragments, and 30-90 parts by weight of fusobacterium nucleatum thalli and thalli fragments are compounded into an antigen to immune an egg laying hen. The invention also discloses the specific preparation method of the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation, and toothpaste containing the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. By the IgY toothpaste which is used for treating and preventing gingivitis and ozostomia has obvious effects in preventing and treating the gingivitis and the ozostomia.

Description

technical field [0001] The present invention relates to IgY antibody and its preparation method and the application of this IgY preparation, especially to anti-Porphyromonas gingivalis and F. Toothpaste for gum inflammation and bad breath caused by two bacteria. Background technique [0002] The incidence of gingival and periodontal diseases accounts for more than 70% of the population in my country, and Porphyromonas gingivalis is the main suspected cause of periodontal diseases such as chronic gingivitis, chronic periodontitis and aggressive periodontitis bacteria, whose pili can bind to glyceraldehyde-3-phosphate dehydrogenase on the surface of oral epithelial cells, invade gingival epithelial cells and gingival fibroblasts, and escape immune clearance. Porphyromonas gingivalis produces dipeptidyl aminopeptidase to degrade connective tissue and promote increased MMP-2 and MMP-1 activity. The arginine- and lysine-specific cysteine ​​proteases (RgpA, RgpB, Kgp) of Porphyro...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K8/64A61K8/81A61K8/86C07K16/12A61P1/02A61Q11/00
Inventor 陈健芬罗鑫龙孙晓青张萍
Owner 上海美加净日化有限公司
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