The present invention relates to a method and composition for generating an artificial
intron and its components capable of producing
microRNA (miRNA) molecules and thus inducing specific
gene silencing effects through
intracellular RNA interference (RNAi) mechanisms, and the relative utilization thereof. The miRNA-producing
intron so generated is not only useful for delivering desired miRNA function into the
intron-mediated transgenic organisms or cells but also useful for suppressing unwanted
gene function in the transgenic organisms or cells thereof. Furthermore, the derivative products of this novel man-made miRNA-producing intron have utilities in probing
gene functions, validating
drug targets, generating transgenic animals and gene-modified plants, developing anti-viral vaccines and treating as well preventing gene-related diseases (gene therapy).