The invention relates to the technical field of
virus detection. The invention provides a
rapid detection technology of high-sensitivity pathogenic
nucleic acid for non-diagnosis and treatment purposes. The
rapid detection technology comprises the following steps of (1) carrying out isothermal amplification on
recombinase; and (2) carrying out color developing by
colloidal gold. According to the
rapid detection technology for the high-sensitivity pathogenic
nucleic acid, isothermal
nucleic acid amplification-
colloidal gold color development is used for
pathogen field detection, special equipment and
reagent are not needed, meanwhile, the
pathogen extraction step is omitted,
pathogen nucleic acid signals can be captured and amplified to the maximum extent,
sputum, blood, excrement and othersamples of a patient can be directly detected, and the technology is very suitable for
field detection, bedside diagnosis of hospitals, crowd gathering and living scenes such as schools and airportsand application under the condition of few other resources, and is particularly suitable for scenes where results need to be obtained rapidly. The defects that in the prior art, pathogen nucleic aciddetection depends on large equipment and
reagent, the detection period is long, the sensitivity is low, and pathogen needs to be extracted can be overcome.