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Magnetic microsphere and application thereof in hair detection

A magnetic microsphere and magnetic separation technology, applied in the field of chemical detection, can solve the problems of large operator influence, large consumption of reagents, cumbersome and time-consuming steps, etc., and achieve less consumption of organic solvents, reduce the influence of human factors, and be environmentally friendly. Effect

Active Publication Date: 2018-07-17
SHANGHAI CRIMINAL SCI TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the shortcomings of the existing detection method, which are cumbersome and time-consuming steps, large reagent consumption, greatly affected by operators, or poor reliability, the present invention proposes a magnetic microsphere and its hair detection application, and synthesizes tetraoxide by improving co-precipitation triiron colloid, and prepare magnetic styrene microspheres by styrene dispersion polymerization, and finally through divinylbenzene and N-vinyl-ε-caprolactam co-modification; the present invention utilizes magnetic solid-phase extraction technology as pretreatment technology, reduces Influenced by human factors, the extraction steps of zepam drugs in hair are improved, with high throughput, less consumption of organic solvents, more friendly to the environment, and high sensitivity

Method used

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  • Magnetic microsphere and application thereof in hair detection
  • Magnetic microsphere and application thereof in hair detection
  • Magnetic microsphere and application thereof in hair detection

Examples

Experimental program
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Effect test

Embodiment 1

[0018] This embodiment specifically includes the following steps: disperse 1.5g of ferrous chloride tetrahydrate and 3g of ferric chloride hexahydrate into a mixture of 200mL of deoxygenated water and 20mL of ethylene glycol, add 2g of polyethylene glycol 300 in Stir mechanically under nitrogen atmosphere for 30 minutes, then add 0.2g poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt (4-styrenesulfonic acid: maleic acid (molar ratio 1:1)), continue The reaction was stirred mechanically for 30 minutes, 30 mL of concentrated ammonia solution was added, and the temperature of the mixed solution was raised to 80° C. to continue the reaction for 1 hour. Gained black precipitate was washed 5 times with deionization, washed twice with ethanol, and finally dispersed in deionized water to prepare a 100 mg / mL colloidal dispersion to prepare 12nm polyethylene glycol 300 and PSSMA co-stabilized tetroxide Triferro colloidal particles.

Embodiment 2

[0020] This embodiment specifically includes the following steps: disperse 2g of ferrous chloride tetrahydrate and 3.5g of ferric chloride hexahydrate into a mixture of 200mL of deoxygenated water and 20mL of ethylene glycol, add 3g of polyethylene glycol 800 in Stir mechanically under nitrogen atmosphere for 30 minutes, then add 0.3g poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt (4-styrenesulfonic acid: maleic acid (molar ratio 1:1)), continue The reaction was stirred mechanically for 30 minutes, 30 mL of concentrated ammonia solution was added, and the temperature of the mixed solution was raised to 80° C. to continue the reaction for 1 hour. Gained black precipitate was washed 5 times with deionization, washed twice with ethanol, and finally dispersed in deionized water to prepare a 100 mg / mL colloidal dispersion to prepare 9nm polyethylene glycol 800 and PSSMA co-stabilized tetroxide Triferro colloidal particles.

Embodiment 3

[0022] This embodiment specifically includes the following steps: disperse 2 g of ferrous chloride tetrahydrate and 4.5 g of ferric chloride hexahydrate into a mixture of 200 mL of deoxygenated water and 20 mL of ethylene glycol, and add 4.5 g of polyethylene glycol 200 Under nitrogen atmosphere, mechanically stirred for 30 minutes, then added 0.4g poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt (4-styrenesulfonic acid: maleic acid (molar ratio 1:1)), Continue the mechanical stirring reaction for 30 minutes, add 30 mL of concentrated ammonia solution, and raise the temperature of the mixed solution to 80° C. to continue the reaction for 1 hour. Gained black precipitate was washed 5 times with deionization, washed twice with ethanol, and finally dispersed in deionized water to prepare a 100mg / mL colloidal dispersion to prepare 7nm polyethylene glycol 2000 and PSSMA co-stabilized tetroxide Triferro colloidal particles.

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Abstract

The invention provides a magnetic microsphere and application thereof in hair detection. After cleaned to-be-detected hair is ground, the magnetic microsphere is added and evenly mixed, and the mixture is dried after magnetic separation; then eluant is added, and the materials are evenly mixed and then subjected to secondary magnetic separation treatment; finally, supernate is obtained for liquidchromatography mass spectrometry to detect the content of oxazepam drugs. The magnetic solid-phase extraction technology is used as a pretreatment technology, so that the influence of human factors isreduced, the extraction step of the oxazepam drugs for hair is improved, the organic solvent consumption is low, the magnetic microsphere is more environmentally friendly, and the sensitivity is high.

Description

technical field [0001] The invention relates to a technique in the field of chemical detection, in particular to a detection method based on magnetic solid-phase extraction technology for extracting azepam from hair. Background technique [0002] The zepam drugs in hair are mainly located in the cuticle and medulla of the hair, and are combined with proteins, so the hair samples need to be pre-treated before the detection of the zepam drugs in the hair. Release the maazepam from the hair while also removing oil, sweat and other interfering substances from the outside of the hair. The choice of pre-treatment technology needs to consider whether the type of analyte and its metabolites will be destroyed during processing, whether the qualitative and quantitative methods used in the follow-up can achieve the purpose of analysis, etc. The current pretreatment of hair samples generally involves contacting the test object with various solvents, which may easily cause the test obje...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26G01N30/02B01J20/28B01J20/30
CPCB01J20/06B01J20/264B01J20/28009G01N30/02
Inventor 杨飞宇倪春芳汪蓉梁晨张玉荣张成功邹芸袁晓亮曹芳琦刘文斌
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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