Excrement nucleic acid methylation purification method

Abstract

The invention provides an excrement nucleic acid methylation purification method. The method includes the following steps that 100 ul of an excrement solution is provided into a centrifugal tube; 1 mL of lysate is added into the centrifugal tube, wherein the lysate is prepared from 0.3N sodium hydroxide, 0.4M potassium chloride, 0.025%N sodium lauroyl sarcosine, 2mM EDTA, 0.4M Tris HCL and 1.5% triton X-100; 100 ul of a conversion solution with the ph value being 5.5 is added into the centrifugal tube, and the centrifugal tube is put in an environment at the constant temperature of 75 DEG C for 90 Min; after the material is cooled to room temperature, 10 ul of a magnetic bead solution is added, and the material is mixed to be uniform and put for 15 Min; the centrifugal tube is put on a magnetic frame for 5 Min, and clean liquid is removed; the second step to the six step are repeated till magnetic beads are separated, and supernate is transferred into another centrifugal tube.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a method for purifying feces nucleic acid methylation. Background technique [0002] Methylation refers to the process of catalytic transfer of methyl groups from reactive methyl compounds such as S-adenosylmethionine to other compounds. Various methyl compounds can be formed, or some proteins or nucleic acids can be chemically modified to form methylated products. In biological systems, methylation is enzymatically catalyzed and involved in heavy metal modification, regulation of gene expression, regulation of protein function, and ribonucleic acid (RNA) processing. [0003] DNA methylation: DNA methylation in vertebrates generally occurs at the CpG site (cytosine-phosphate-guanine site, that is, the site where cytosine is immediately followed by guanine in the DNA sequence). DNA methyltransferase catalyzes the conversion of cytosine to 5-methylcytosine. About 80%-90% of CpG ...

AI Technical Summary

Problems solved by technology

[0005] At present, the automated nucleic acid extraction process includes: sample lysis, magnetic bead adsorption, washing and purification, and nucleic acid elution. The process is complicated and there are many steps, resulting in more loss of DNA for the processed gene.