362 results about "Triton X-100" patented technology

Methods for decellularizing mammalian tissue for use in transplantation and tissue engineering. The invention includes methods for simultaneous application of an ionic detergent and a nonionic detergent for a long time period, which may exceed five days. One method utilizes SDS as the ionic detergent and Triton-X 100 as the nonionic detergent. A long rinse step follows, which may also exceed five days in length. This long duration, simultaneous extraction with two detergents produced tissue showing stress-strain curves and DSC data similar to that of fresh, unprocessed tissue. The processed tissue is largely devoid of cells, has the underlying structure essentially intact, and also shows a significantly improved inflammatory response relative to fresh tissue, even without glutaraldehyde fixation. Significantly reduced in situ calcification has also been demonstrated relative to glutaraldehyde fixed tissue. Applicants believe the ionic and non-ionic detergents may act synergistically to bind protein to the ionic detergent and may remove an ionic detergent-protein complex from the tissue using the non-ionic detergent. The present methods find one exemplary use in decellularizing porcine heart valve leaflet and wall tissue for use in transplantation.

The present disclosure is drawn to pharmaceutical compositions and oral dosage forms containing testosterone undecanoate, as well as related methods of treatment. In one embodiment, the oral dosage form can include a therapeutically effective amount of testosterone undecanoate and a pharmaceutically acceptable carrier. The dosage form can be formulated such that, when measured using a USP Type II apparatus in 1000 mL of 8 wt % Triton X-100 in water at 37° C. and 100 rpm, the oral dosage form releases at least 20% more testosterone undecanoate after the first 120 minutes than an equivalent dose testosterone undecanoate containing oral dosage form without the pharmaceutically acceptable carrier.

The present invention relates to a preparation method of titanium dioxide visible photocatalyst in which the carbon and nitrogen are doped. Said method includes the following steps: firstly, adding nitrogen source into the microemulsion formed from n-butyl titanate, inorganic acid, Triton X-100, n-hexyl alcohol and cyclohexane and making mole ratio value of nitrogen and titanium be 0.5-4, then placing the nitrogen-contained microemulsion in a reactor, making hydrothermal reaction for 13 hr at 120deg.C, finally washing the product obtained after hydrothermal reaction, separating and drying so as to obtain the invented target product.

The invention belongs to a method for preparing a nano ternary composite lithium ion anode material by utilizing a microemulsion. The method comprises the following steps: firstly, mixing a surfactant triton X-100 and a cosurfactant n-butyl alcohol with cyclohexane so as to prepare an emulgator; secondly, dissolving nickel salt, cobalt salt and manganese salt into deionized water; thirdly, preparing nickel, cobalt and manganese salt microemulsion; fourthly, preparing complexing agent aqueous alkali; fifthly, preparing alkali microemulsion; sixthly, adding the nickel, cobalt and manganese salt microemulsion into a reaction kettle; seventhly, adding alkali microemulsion dropwise into the nickel, cobalt and manganese salt microemulsion so as to prepare a turbidimetric solution; eighthly, standing the turbidimetric solution still so as to prepare a NixCoyMn(1-x-y)(OH)2 precursor; ninthly, carrying out ball-milling on the NixCoyMn(1-x-y)(OH)2 precursor and a lithium compound, mixing uniformly, and sieving; and tenthly, preparing the sieved mixture into a nano spherical LiNixCoyMn(1-x-y)(OH)2 anode material. The nano ternary composite lithium ion anode material has favorable cycle stability.

The invention provides a lysis solution for extracting nucleic acid through a magnetic bead method. The lysis solution is prepared from 0.2-0.4 N sodium hydroxide, 0.3-0.6 M potassium chloride, 0.01-0.05% sodium n-lauroyl sarcosinate, 5mM EDTA, 0.3-0.6 M Tris-HCL and 1-2% triton X-100. The lysis solution for extracting nucleic acid through the magnetic bead method can fully and effectively lyse cells to accelerate the lysis progress and also can protect nucleic acid against oxidation to prevent formation of DNA dimmers. Besides, the lysis solution is stable in property and free of influences of seasons, temperature, saline ion concentration and the like and can carry out nucleic acid adsorption by fully cooperating with magnetic beads to achieve the optimal nucleic acid extraction effect. The scrubbing solution for extracting nucleic acid through the magnetic bead method can effectively remove residual impurities and avoid loss of nucleic acid, and a small quantity of the residual scrubbing solution does not influence the PCR application effect, so that the accuracy of detection results is guaranteed.

The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W / V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.

The invention relates to a method for detecting the microelement content of liquid milk or milk powder. The method comprises the steps as follows: firstly, a standard curve is made; secondly, a sample is detected; thirdly, the detected result of the sample is compared with the standard curve, and the content of the microelement in the sample is obtained; and a detection apparatus is an inductive coupling plasma emission spectrometer. Through the diffusion function of cesium chloride, the surface activity of the sample is changed by utilizing triton x-100, and the sample is added to beryllium standard liquid to be compared, and atomized through an instrument atomizing chamber and is quantified by adopting a multi-reaction monitoring mode. The instrument detection limit of the method is 0.024 to 0.21 Mu g / ml, in an element linear range, a correlation coefficient r is above 0.999, and the recovery rate is 86.8 percent to 103.8 percent. The method has the advantages that the content of microelements in the liquid milk and the milk powder can be rapidly and accurately measured by the method, the probability of the element loss and the element pollution in the sample preparation process is low, results are accurate and reliable, the repeatability is good, and the sensitivity is high.

The invention discloses a preparation method for the molecularly imprinted-quantum dot polymer of a pyrethroid type pesticide, which is characterized by comprising the steps of (1) adding 1.8 mL of Triton X-100 in 7.5 mL of cyclohexane, magnetically stirring the obtained solution, dripping 400 mu L of quantum dots, 20-50 mu L of cross-linking agent and 100 mu L of ammoniacal liquor drop by drop, magnetically stirring the obtained solution for 2 hours, then adding 200 mu L of 25 mg / mL template molecular solution and functional monomers, and stirring the obtained solution for 8-12 hours at the room temperature; (2) after the completion of the above reaction, adding 10 mL of acetone for precipitation, centrifuging the obtained product at 9000 rpm for 10 mins, removing the supernatant, adding 6 mL of double distilled water to disperse precipitates, and then centrifuging the obtained product at 9000 rpm for 15 mins, removing the supernatant, adding a mixed liquor of ethanol and acetonitrile in the volume ratio of 8:2, extracting for 2 hours, removing template molecules and obtaining a finished product through the vacuum drying process. The method is high in sensitivity, good in stability and high in selectivity.

The invention relates to a chemiluminescent detection reagent kit based on microparticles. The chemiluminescent detection reagent kit comprises a vector coated with an antibody, an antigen or Protein A, a labelled antibody, a standard product of a determinant, a washing solution and a luminous solution, wherein the vector coated with the antibody, the antigen or the Protein A is a microparticle containing groups of -COOH, -NH2, -OH or -CHO; and the antibody, the antigen or the Protein A is coated on the surface of the microparticle in a chemical crosslinking mode. The labelled antibody is an antibody which is connected with acridinium ester or a derivant of the acridinium ester in the chemical crosslinking mode. The luminous solution comprises a hydrogen oxide and nitric acid aqueous solution and a sodium hydroxide and Triton X-100 aqueous solution. The microparticle can suspend on the sample solution, which is favorable for increasing full contact between the antibody, the antigen or the Protein A on the surface of the microparticle and the determinant and shortening the reaction time. The acridinium ester or the derivant of the acridinium ester is used as a label and can obtain a stable and high-strength signal.

The present invention relates to a method for separating metals by micellar ultrafiltration, that can be used to process radioactive waste. This process consists of adding to the aqueous solution a complexing anionic surfactant having the formula: M+=cation, for example NA+m=1 or 2 R=aliphatic group of at least eight carbon atoms, and optionally a nonionic surfactant such as Triton X-100 or Brij 35 to produce micelles (10) in the solution, and subsequently of filtering the solution through an ultrafiltration membrane (3) to retain the previously formed micelles.

The invention provides a method of preparing molybdenum disulfide microspheres through sulfurizing and reducing a soluble molybdenum source in a reversed-phase microemulsion system. The method includes a) a step of preparing a reversed-phase microemulsion A through adopting polyethylene glycol octylphenol ether (Triton X-100), n-hexanol, cyclohexane and an aqueous solution of the soluble molybdenum source as a surfactant, a cosurfactant, an oil phase and a water base respectively, b) a step of adding an aqueous solution containing a sulfur source and a reductant into the reversed-phase microemulsion A to obtain a reversed-phase microemulsion B, and c) a step of transferring the reversed-phase microemulsion B to a hydrothermal reactor, performing crystallization thermal treatment at a certain temperature for a certain period of time, separating, washing, and drying the reaction product to obtain the molybdenum disulfide microspheres. The method is simple and mild in reaction conditions. Through characterization by a scanning electron microscope and a transmission electron microscope, the molybdenum disulfide microspheres have the characteristics of being solid or hollow, smooth or wrinkled in surface, and the like.

The invention discloses a method for preparing a fiber film with structural color, which comprises preparing colloidal emulsion containing polymer microspheres; adding a coupling agent and Triton X-100, and completely mixing to obtain a mixed solution; electrospinning the mixed solution to obtain colloidal crystal fiber; and removing the coupling agent in the colloidal crystal fiber to obtain the fiber film with structural color. The invention expands coherent diffraction between light and a planar interface of a photonic crystal slab to a cylindrical interface of photonic crystal fiber, forms the photonic crystal fiber by using colloidal nanospheres via self-assembly, and prepares environmentally-friendly chemical dye-free fiber with structural color. The method of the invention has advantages of simple raw material, low cost and large-scale preparation, is free from being influenced by viewing angles, and is independent of reflection of environmental light.

The invention discloses a microenvironment construction method for three dimensional culture of cells based on a specificity extracellular matrix and an application. A specificity extracellular matrix extraction method, the specificity extracellular matrix and a photocuring material are combined to prepare hydrogel, and the invention relates to an application of the hydrogel to in vitro three dimensional culture and particularly an organ chip technique. According to the technical scheme, in accordance with tissue or organs of different density, surfactants having different decellularization capacity are selected, through combination of the manner that enzyme treatment is selected and an ammonia solution is used as a solvent, particularly-compact tissue or organs are treated, and the preferred combination of the enzyme and surfactants comprises Trypsin and a Triton X-100 solution, and the solvent is a 0.05%-0.5% ammonia solution. The extracellular matrix and a light-initiating colloid-forming material are compounded for preparing the hydrogel so as to construct cell culture microenvironment. The method has the advantages of being high in colloid-forming speed, simple in colloid-forming condition, controllable in hydrogel mechanical property and the like.

The invention discloses a yolk-eggshell structure Au@ hollow carbon nanosphere composite material and preparation and application thereof. A preparation method includes the following steps that sodium citrate is adopted for reducing chlorogoldacid, and nanogold hydrosol is prepared; the nanogold hydrosol, organic monomers, water and a Triton X-100 aqueous solution are stirred and mixed; then, an initiating agent is added into a system to initiate polymerization of the monomers, and precursors are prepared; the organic monomers are composed of aniline and pyrrole; and under the inert atmosphere, the precursors are processed in a high-temperature carbonizing manner so that the yolk-eggshell structure Au@ hollow carbon nanosphere composite material can be prepared. According to the preparation method, the tedious steps that a template is prepared, complicated surface modification is needed and the template is removed are not needed, and preparation is easy and convenient. The specific surface area and the size of the prepared material can be regulated and controlled through the carbonization condition and the concentration of the organic monomers, the specific surface area is high, and the prepared material has a good catalytic effect on nitrobenzene and p-nitrophenol.

The invention relates to an infrared phase-changing material, which relates to an optoelectronic material. The infrared phase-changing material comprises an inner shell, an outer shell, and a core material, the inner shell comprises a copolymer containing styrene and methylacrylic acid, the outer shell comprises conductive polyaniline, and the core material comprises various kinds of paraffins. The preparation method comprises the following steps: uniformly mixing the paraffins, styrene, methylacrylic acid, dodecyl mercaptan and azobisisobutyronitrile according to a certain proportion; dissolving a certain amount of sodium dodecyl sulfate and TritonX-100 in de-ionized water; completely mixing the two solutions together, moving the solutions in a four-hole bottle after ultrasonic emulsification in ice-bath, filling nitrogen, heating and agitating; after six hours, obtaining a powder sample through cooling, emulsion breaking, filtration, washing and drying; uniformly mixing a certain proportion of powder sample, aniline monomer and dilute hydrochloric acid together, and slowly adding a certain amount of ammonium persulfate and hydrochloric acid solution after cooling down; and after six hours, obtaining the final product through filtration, washing and drying. The invention solves the problem that two materials are needed for the temperature control and the reduction of the infrared emissivity, increases the associative property of different materials, and reduces the coating thickness and the actual construction links.

An air dehydration membrane is made of a hydrophilic polymer having a permeability for water vapor which is greater than its permeability for air, and having low selectivity between oxygen and nitrogen. The membrane has a hydrophilic coating, which itself may be a polymer. The coating does not affect the selectivity of the coated membrane with respect to oxygen and nitrogen, but does increase selectivity of the membrane with respect to water vapor. A preferred material for the membrane is polysulfone. Preferred materials for the coating are poly vinyl alcohol and Triton X-100. The membrane is selected such that the dominant mechanism for gas flow through the membrane is Knudsen flow. The membranes are made at low cost, and can outperform existing commercial membranes in either volumetric productivity or product recovery.

Decellularized tissue-derived extracellular matrices (ECM) and methods of generating and using same are provided. The method of generating a decellularized matrix includes the steps of: (a) subjecting the tissue to washes and a hypertonic buffer; (b) subjecting the tissue to an enzymatic proteolytic digestion with an enzyme such as trypsin; and (c) removing all cellular components from the tissue using a detergent solution which includes Triton-X-100 and ammonium hydroxide. Specifically, there is provided a decellularized myocardium-derived matrix which is completely devoid of all cellular components and hence non-immunogenic in a subject, exhibits suitable structural and mechanical properties for cardiac tissue engineering or replacement therapy of damaged cardiac tissue, and is capable of remodeling upon seeding of cells.

The invention relates to a neuron-specific enolase stabilizer. The stabilizer is prepared from a Tris.HCl buffer solution, NaCl, sucrose, trehalose, casein, bovine serum albumin, PEG6000, lysine, disodium ethylene diamine tetraacetate, dithiothreitol, glycerin, Tween-20, Triton X-100, aprotinin with the final concentration of 100-300 KIU / mL, and Proclin 300, and above substances are uniformly mixed in Millipore ultrapure water used as a solvent, is filtered by a 0.22 [mu]m filter membrane, and finally is preserved at 0-10 DEG C. The stabilizer greatly improves the preservation stability of neuron-specific enolase, prolongs the shelf life of a kit, simplification of the production technology and the doctor's detection operation process of a neuron-specific enolase detection kit, improvement of the metamorphism resistance in the preparation, transportation and storage process of the neuron-specific enolase, and reduction of inter-assay variation caused by a freeze-drying technology.

The invention relates to a fluorescent dyeing method for observing the microstructure of plants, which can effectively solve the problem of rapid film production and observation of the microstructure of plants. , the plant material was fixed in paraformaldehyde or methanol with a mass concentration of 4% configured by pH 8.0 PBS buffer solution for 20-40min, rinsed with pH 8.0 PBS buffer solution for 10-20min, and prepared by DMSO, Triton X-100 and fluorescent whitening agent 220 make staining solution, stain at room temperature for 10-60min, then rinse with PBS buffer for 10-15min, add DAPI solution dropwise for fluorescent staining of nucleic acid in cells, rinse with distilled water, and then add dropwise Glycerin, add a cover glass, observe and take pictures with a laser confocal microscope, the method of the present invention is simple, does not need a microtome, and the production time is short, and the three-dimensional structure of the plant can be observed, which is suitable for the observation of cells or tissues of various plants, clear , accurate, low cost and good effect.

The invention provides a lithium-ion-battery multiwalled carbon nanotube / molybdenum disulfide combination electrode and a preparation method. According to the preparation method, multiwalled carbon nanotube, soluble molybdate and L-cysteine are adopted as materials, glucose and triton X-100 are used as additives, dispersion uniformity of MoS2 on the surface of the carbon nanotube is improved, and through an in-situ hydrothermal reduction method, a combination electrode material of MoS2 and the multiwalled carbon nanotube is synthesized. The material has high electrochemical storage lithium specific capacity, excellent cycle performance and a high-rate feature. Meanwhile, the synthetic method has the advantages of being moderate in reaction condition, simple in process, high in yield and good in repeatability.

The invention relates to a method for preparing the conspecific allo vessel de-cell support, which comprises that using the abdominal aorta of rabbit of New Zealand, using surface activator Triton X-100 to extract the liposome of cell membrane, and digesting the parenzyme, using nuclease DNAse and RNAse to degrade the DNA and RNA of cell, to obtain the support. The invention uses the similar structures of conspecific allo materials, with better histocompatibility, to support healing after surgery, with simple preparation and high efficiency. And the invention can be transplanted with low caused immunity reaction of host, to supply physical strength and tension. The invention can be used to replace blood vessel.

An immune-fluorescence labeling method of micro-pipe frame at pollen tube on gymnosperm includes fixing pollen tube of gymnosperm on fixing liquid for 40-60min, washing pollen tube, placing pollen tube in enzyme solution for carrying out enzymolysis of 30-40 min under temperature of 20-30deg.c, washing pollen tube, setting pollen tube in Triton X-100 to penetrate there for 1-3h, washing pollen tube, adding anti-beta-tubulin single clone antibody to carry out hatching for 1-3h, washing pollen tube, adding fluorescence label to carry out shielded hatching for 1-3h for making micro-pipe frame be immune-fluorescence labeled.

The invention relates to a method for intensively removing hexachloro-cyclohexane soprocide and dichlorodiphenyl trichloroethane in soil by zero-valent iron. The method comprises the following steps: immersing reduced iron powder into a copper solution to prepare a micron Fe / Cu duplex metal material, so that the load amount of Cu is 1%; sequentially adding duplex metal and triton X-100 into the soil according to the adding amounts of 5% and 0.1%; adjusting the initial pH value of the soil to 4-5 and adjusting the water content rate of the soil to 25%-30%; and isolating oxygen and piling to react for a certain time so as to degrade and remove hexachloro-cyclohexane soprocide and dichlorodiphenyl trichloroethane in the soil. The method does not need to use complicated equipment, is simple to operate and environment-friendly; the method has economical cost and has no secondary pollution; and the method has a relatively good application prospect in the remediation of the soil polluted by low-medium concentration hexachloro-cyclohexane soprocide and dichlorodiphenyl trichloroethane.

The invention relates to an infrared magnetic phase change material, which belongs to an optoelectronic material. The structure of the infrared magnetic phase change material is as follows: an inner shell (2) is a copolymer of styrene and methacrylic acid, an outer shell (1) is made of conductive polyaniline, a core material B is paraffin (4), and a core material A is modified nano-ferrite (3). Apreparation method comprises the following steps: mixing ferrite, paraffin, styrene, methacrylic acid, lauryl mercaptan and azodiisobutyronitrile according to a certain proportion; dissolving a certain amount of lauryl sodium sulfate and Triton X-100 in deionized water; mixing the two kinds of solution for emulsification, filling nitrogen, heating and mixing; after 6 hours, breaking emulsion, leaching, washing, drying, mixing the obtained powder with aniline monomer and dilute hydrochloric acid according to a certain proportion, reducing the temperature, and dropping hydrochloric acid solution containing a certain amount of ammonium persulfate; and after 6 hours, leaching, washing and drying, thereby obtaining the final product. The invention integrates three functions within one material, i.e. microwave absorption, temperature control and reduction of infrared emission, and solves the problem that various materials are needed in the conventional multi-band protection.

The invention relates to the field of aquatic product detection, and discloses a method for detecting the heavy metal content of an aquatic product. The method comprises the following steps: 1), pretreating: removing inedible inner organs, fish skin, fish scales, fish bones and other hard tissues of the aquatic product, then crushing remaining tissues into minced meat, adding an aqueous Triton X-100 solution and a magnetic heavy metal adsorbent, mixing and stirring; 2), performing heavy metal extraction; 3), calibrating a standard curve; 4), performing heavy metal content detection. By the heavy metal detection method, strong acid and a microwave digestion instrument are not required to be used for digestion, operation is simple, safety and environment friendliness are achieved, the effectof enriching heavy metals in the aquatic product is good, the magnetic heavy metal adsorbent can be reused, remaining impurities of the aquatic product have no influence on a detection sample duringthe detection, and a detection result is accurate, so that the heavy metal detection method is a very accurate, efficient and environment-friendly detection method and has a practical significance inimproving the food safety.

The invention discloses compound treatment agents for oily sludge containing polymers and a usage method thereof, and belongs to the technical field of polymer-containing oily sludge treatment in petroleum oilfield. The compound treatment agents for the oily sludge containing the polymers comprise an agent A, an agent B, an agent C and an agent D; the agent A is composed of sodium carbonate, sodium silicate and sodium hydroxide; the agent B is composed of an OP emulsifier, Peregal, Triton X-100 and sodium dodecyl benzene sulfonate; the agent C is composed of sodium hypochlorite and hydrogen peroxide; and the agent D is composed of aluminum sulfate, sodium sulfate and polyferric sulfate. The usage method of the compound treatment agents for the oily sludge containing the polymers comprisesthe following steps: a, putting oily sludge containing polymers into a reaction kettle, adding water, and carrying out heating with stirring; b, adding the agent A and carrying out uniform stirring, adding the agent B and carrying out uniform stirring, adding the agent C and carrying out uniform stirring, and adding the agent D and carrying out uniform stirring; and c, performing standing-precipitating on the mixture of the oily sludge and the water, and separately retrieving oil, sludge and water after stratification. The compound treatment agents for the oily sludge containing the polymers and the usage method thereof are capable of realizing effective separation on the three phases of oil, sludge and water in the oily sludge containing the polymers so as to achieve reduction and resource recycling; and thus, the problem of performing harmless treatment and resource recycling on hazardous solid waste from oilfield is effectively solved.