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Preparation method of allogenic blood vessel decellularised scaffold

A decellularized scaffold and allogeneic technology, which is applied in the field of preparation of allogeneic vascular decellularized scaffolds, can solve the problems of limited sources of autologous blood vessels, low long-term patency rate, and inability to guarantee clinical use, etc., to achieve cell adhesion, Good tissue compatibility and biodegradability, favorable exchange effect

Inactive Publication Date: 2007-08-08
THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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AI Technical Summary

Problems solved by technology

At present, the sources of available autologous blood vessels are limited, and most operations cannot be performed at all; artificial blood vessels, especially artificial blood vessels with a diameter of less than 4-6 mm, have low short-term and long-term patency rates and cannot be used clinically.

Method used

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  • Preparation method of allogenic blood vessel decellularised scaffold
  • Preparation method of allogenic blood vessel decellularised scaffold
  • Preparation method of allogenic blood vessel decellularised scaffold

Examples

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Embodiment Construction

[0035] Take the abdominal aorta of New Zealand white rabbits, about 1.0cm in length, soft in texture and good in compliance, as shown in Figure 1; soak in TBS at 4°C for 2h; secondly, use surfactant 1% TritonX-100 at 4°C for 12h to extract The lipid component of the cell membrane destroys the cell membrane structure; then use trypsin to digest the allogeneic blood vessels, that is, 0.125% trypsin is digested at 37°C for 12 hours to degrade part of the tissue, improve tissue permeability, and facilitate reagents to enter the interior of the cell Then use nuclease DNAse, RNAse to degrade various DNA and RNA components in the cell, that is, digest 12h at 37°C with 0.5mg / ml DNase and 0.5mg / ml RNase at a ratio of 1:1, and wash 3 times with TBS to reach The purpose of complete removal of cells. Such processed allograft blood vessels can be used for arterial grafting instead of the removed arterial blood vessels.

[0036] During the transplantation operation, New Zealand white rabbi...

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Abstract

The invention relates to a method for preparing the conspecific allo vessel de-cell support, which comprises that using the abdominal aorta of rabbit of New Zealand, using surface activator Triton X-100 to extract the liposome of cell membrane, and digesting the parenzyme, using nuclease DNAse and RNAse to degrade the DNA and RNA of cell, to obtain the support. The invention uses the similar structures of conspecific allo materials, with better histocompatibility, to support healing after surgery, with simple preparation and high efficiency. And the invention can be transplanted with low caused immunity reaction of host, to supply physical strength and tension. The invention can be used to replace blood vessel.

Description

technical field [0001] The invention relates to a preparation method of a blood vessel decellularized scaffold, in particular to a preparation method of an allogeneic blood vessel decellularized scaffold. Background technique [0002] Arterial ischemic diseases, mainly represented by arteriosclerotic vascular diseases, are an important cause of death. [0003] Before the present invention, arterial bypass surgery was one of the main methods for treating arteriosclerotic vascular diseases, that is, using autologous blood vessels or artificial blood vessels as graft materials to perform bypass surgery. At present, the sources of available autologous blood vessels are limited, and most operations cannot be performed at all; artificial blood vessels, especially those with a diameter of less than 4-6 mm, have low short-term and long-term patency rates and cannot be used clinically. Contents of the invention [0004] The purpose of the present invention is to overcome the above...

Claims

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Application Information

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IPC IPC(8): A61L29/00
Inventor 冉峰刘长建周敏夏成勇
Owner THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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