102 results about "Tissue reconstruction" patented technology

A material fixation system is particularly adapted to improve the tendon-to-bone fixation of hamstring autografts, as well as other soft tissue ACL reconstruction techniques. The system is easy to use, requires no additional accessories, uses only a single drill hole, and can be implanted by one person. Additionally, it replicates the native ACL by compressing the tendons against the aperture of the tibial tunnel, which leads to a shorter graft and increased graft stiffness. It is adapted to accommodate single or double tendon bundle autografts or allografts. It also provides pull out strength measured to be greater than 1000 N.

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

Methods for decellularizing mammalian tissue for use in transplantation and tissue engineering. The invention includes methods for simultaneous application of an ionic detergent and a nonionic detergent for a long time period, which may exceed five days. One method utilizes SDS as the ionic detergent and Triton-X 100 as the nonionic detergent. A long rinse step follows, which may also exceed five days in length. This long duration, simultaneous extraction with two detergents produced tissue showing stress-strain curves and DSC data similar to that of fresh, unprocessed tissue. The processed tissue is largely devoid of cells, has the underlying structure essentially intact, and also shows a significantly improved inflammatory response relative to fresh tissue, even without glutaraldehyde fixation. Significantly reduced in situ calcification has also been demonstrated relative to glutaraldehyde fixed tissue. Applicants believe the ionic and non-ionic detergents may act synergistically to bind protein to the ionic detergent and may remove an ionic detergent-protein complex from the tissue using the non-ionic detergent. The present methods find one exemplary use in decellularizing porcine heart valve leaflet and wall tissue for use in transplantation.

A tissue cell growth-promoting solution produced by this invention comprising water containing at least 1 to 500 ppm of active oxygen, when applied to a wound, supplies active oxygen originating from outside the biobody to supplement the active oxygen produced by the biobody's own protective system cells such as neutrophils and macrophages which gather at the wound site, thus increasing the concentration of active oxygen at the site of the wound, mimicking a state in which a large quantity of such bio-signals is secreted by the biobody itself, to promote the reconstruction of tissues, the action corresponding to the last of the four main steps involved in wound healing biochemical processes of "blood vessel reaction", "blood vessel coagulation", "inflammation", "reconstruction of tissues" and which would otherwise have to rely on the natural healing power of the biobody itself.

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

The invention belongs to the technical field of biological materials and tissue repair and particularly relates to a multi-layer porous scaffold and a preparation method thereof. A suitable porous material is selected, after being clipped according to set size and requirements, the material is bonded by pore-forming adhesive which contains mixture of components of polymer / pore-forming particles / solvent, and pore-forming agent is removed after solidification and bonding, thus preparing the porous scaffold which has multi-layer structures communicated with each other. The scaffold has multi-layer structures, porous transition layers are among the layers of the scaffold, and the pores in an upper layer and a lower layer of the scaffold are communicated with each other; and the scaffold is suitable for transition of different cells and fusion of tissues in a process of tissue remodeling, and weakened separation between layers of the remolding tissue is avoided. The scaffold is suitable for a bionic three-dimensional cell scaffold which repairs a multi-layer tissue and other application fields.

An aromatic polyanhydride having a repeating unit with structure (I) wherein Ar is a substituted or unsubstituted aromatic ring and R is a difunctional organic moiety substituted on each Ar ortho to the anhydride group. Ortho-substituted bis-aromatic dicarboxylic acid anhydride monomers and ortho-substituted bis-aromatic dicarboxylic acid intermediates thereof are also disclosed, as well as implantable medical devices, such as scaffolding implants for tissue reconstruction, drug delivery systems prepared from the aromatic polyanhydrides, as well as therapeutic oral dosage forms and treatment methods.

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

A porous scaffold is disclosed, the porous scaffold comprising electrospun polymeric nanofibers, wherein an average diameter of a pore of the porous scaffold is about 300 μm is disclosed. An average diameter of the polymeric nanofibers ranges from about 100 to 400 nm. The scaffold may comprise a plurality of particles, the particles being greater than about 1 μm in diameter. Methods of fabricating scaffolds, methods for generating tissue and methods of using scaffolds for tissue reconstruction are also disclosed.

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

This invention describes methods for the creation of 3D biologically inspired tissue engineered scaffolds with both excellent interfacial mechanical properties, and biocompatibility and products created using such methods. In some cases, a combination of nanomaterials, nano / microfabrication methods and 3D printing can be employed to create structures that promote tissue reconstruction and / or production. In other embodiments, electrospinning techniques can be used to create structures made of polymers and nanotubes.

The present invention is directed to methods of manufacturing bioactive gels from ECM material, i.e., gels which retain bioactivity, and can serve as scaffolds for preclinical and clinical tissue engineering and regenerative medicine approaches to tissue reconstruction. The manufacturing methods take advantage of a new recognition that bioactive gels from ECM material can be created by digesting particularized ECM material in an alkaline environment and neutralizing to provide bioactive gels.

The invention belongs to the technical field of bomedical material preparation, in particular relates to human tissue engineering support loaded with controlled-release cell growth factor and provided with hollow silicon dioxide ball with kernel and a preparation method and applications thereofThe human tissue engineering support is prepared in the following steps: the hollow silicon dioxide submicron ball loaded with cell growth factors and provided with kernel is self-assembled with gelatin to form a compound ball which is composed of the hollow silicon dioxide submicron ball loaded with cell growth factors and provided with kernel and the gelatin; then the compound ball is loaded to macromolecule polymer fiber base material obtained by the electric spinning method, thus obtaining the human tissue engineering support.With hollow silicon dioxide submicron ball with kernel as a slow release carrier of the cell growth factors, cell proliferation and differentiation is promoted by loading and slowly releasing the cell growth factors.The human tissue engineering support of the invention is an all-round imitation of the cell growth microenvironment, thus enjoying broad application prospect in the field of tissue engineering support covering tissue repair and tissue reconstruction.

Base materials for tissue regeneration which enable the tissue regeneration, have mechanical properties needed in the tissue regeneration and can disappear via degradation in vivo after the completion of the tissue regeneration; and implantable materials with the use of the same. The above-described base materials for tissue regeneration comprise polyrotaxane, wherein biocompatible groups having bulky substituents have been introduced via hydrolyzable bond into both ends of a linear molecule penetrating plural cyclic molecules, or a polyrotaxane hydrogel having a network structure formed by crosslinking the cyclic molecules to each other, the biocompatible groups to each other, or the cyclic molecules to the biocompatible groups in each polyrotaxane molecule.

The present invention relates to a cell construct cultured in vitro characterized in that it comprises (i) animal cells cultured in vitro in conditions ensuring formation of a three-dimensional tissue structure and (ii) an endogenous extracellular matrix in which said cells are imprisoned, and in that it comprises, among said cells, cells having a mineralization and / or ossification capacity. The invention is particularly useful in the fields of grafts and implantology (tissue reconstruction or repair), as well as in the pharmaceutical industry, for research on tissue and analysis of the toxic or beneficial profile of molecules capable of being used in pharmaceutics development and / or pharmaceutical compositions.

The present invention provides an implantable prosthesis comprising a viscera separating barrier that defines a shell having a pocket with an opening that receives an insert. It is contemplated that the insert comprises at least one of an absorbable mesh, a non-absorbable mesh, or an absorbable and non-absorbable mesh combination inserted into the pocket through the opening. In preferred embodiments the viscera separating barrier is acellular collagen, and the absorbable mesh is selected from at least one of a polyglactin, a polyglycolic acid, a polyglactin and a polylactic acid, and the non-absorbable mesh comprises a polypropylene mesh. It is contemplated that the viscera separating barrier acts to reduce or even eliminate attachment of viscera to the prosthesis to encourage rapid cell penetration and revascularization; the non-absorbable mesh will provide immediate, reliable in-growth; and the absorbable mesh will give stability to the prosthesis.

A method and apparatus is provided for creating an internal reconstruction tissue graft. Templates may be used to create a multitude of patterns in a variety of tissue reconstruction grafts. An apparatus may be used to create an internal tissue graft for reconstruction through either compression and / or removal of segments. An apparatus may be used, through either compression and or removal of segments of a preformed template made of synthetics and or metal that mirrors a template that can be used as an internal tissue graft for reconstruction. In a method, such as using software analysis and an apparatus, the physical properties of the tissue graft and its pre- and post-operative properties and appearance may be measured.

The invention discloses a preparation method of a composite soft-tissue patch. Sustained-release microspheres containing human cell culture secretion are mixed with gel solution, then the mixture is compounded with an acellular dermal matrix under vacuum, and after drying and sterilization, the composite soft-tissue patch is obtained. The prepared composite patch can improve the biocompatibility and the bioactivity of the dermal matrix effectively. The experiment shows that compared with the single acellular dermal matrix, the patch can promote cell proliferation and collagen formation effectively and has better biocompatibility and better tissue remolding. The patch can be widely applied to closure of various wound surfaces, repair of various soft-tissue defects and reinforcement of weakplaces and can be prepared into micro particles to be used for filling various fine and weak or dented places by injection, thus having wider application prospect in clinic.

A method and apparatus is provided for creating an internal reconstruction tissue graft. Templates may be used to create a multitude of patterns in a variety of tissue reconstruction grafts. An apparatus may be used to create an internal tissue graft for reconstruction through either compression and / or removal of segments. An apparatus may be used, through either compression and or removal of segments of a preformed template made of synthetics and or metal that mirrors a template that can be used as an internal tissue graft for reconstruction. In a method, such as using software analysis and an apparatus, the physical properties of the tissue graft and its pre- and post-operative properties and appearance may be measured.

The invention discloses a method for preparing a bacterial cellulose compounded amnion extracellular matrix material containing collagen, and relates to a preparation technology of tissue engineering biomaterials. The method comprises the following steps: activating a strain capable of secreting bacterial cellulose to prepare a seed mash; mixing the seed mash with strain concentration of 30-50wt% and a culture medium containing a collagen solution, and putting the mixture into a culture container to stand and culture to obtain a bacterial cellulose / collagen film; immersing an amnion extracellular matrix film in the culture medium, laying the amnion extracellular matrix film on the surface of the bacterial cellulose / collagen film after the amnion extracellular matrix film is completely wet; further standing and culturing for 1-6 hours, purifying and drying to obtain the bacterial cellulose compounded amnion extracellular matrix material containing collagen. The method is simple and practical in preparation process, low in cost and wide in material sources; the prepared compound material has an excellent spatial three-dimensional network, good biocompatibility and high mechanical strength, and can be used as a tissue engineering scaffold material and applied to tissue reconstruction.

The present invention relates to the field of implants. In particular, the present invention relates to an implant for tissue reconstruction which comprises a scaffold structure that includes a void system for the generation of prevascularized connective tissue with void spaces for cell and / or tissue transplantation. Moreover, the present invention relates to a method of manufacturing such an implant, to the internal architecture of such an implant, to a removal tool for mechanical removal of space-occupying structures from such an implant, to a kit comprising such an implant and such a removal tool, to a removal device for the removal of superparamagnetic or ferromagnetic space-occupying structures from such an implant, as well as to a guiding device for providing feedback to a surgeon during the procedure of introducing transplantation cells into the void spaces generated upon removal of space-occupying structures from such an implant.

The invention belongs to the technical field of medical biological materials and discloses a compound biological patch for repairing abdominal wall defects. The compound biological patch is prepared from a biological patch layer and a bletilla striata fiber mesh layer which covers the surface of one side of the biological patch layer, wherein the bletilla striata fiber mesh layer is an electrospunnano-grade bletilla striata fiber mesh. According to the compound biological patch disclosed by the invention, the bletilla striata fiber mesh layer is combined with a traditional biological patch coating film, so that the anti-infection capability and the wound repairing capability of the compound biological patch are effectively improved; meanwhile, after the compound biological patch is implanted into an organism, the mechanical strength change is higher than needed mechanical strength all the time and tissue reconstruction can be accelerated; existing technical bottlenecks of the biological patch are conducive to solving and the treatment effect of repairing the abdominal wall defects is effectively improved.

The invention relates to an amnion-based biological material and a preparation method and uses thereof, and relates to a biological material. The amnion-based biological material is a de-epithelialized amniotic tissue. The preparation method of the amnion-based biological material i.e. the de-epithelialized amniotic tissue comprises the following steps that: the amniotic tissue is de-epithelialized to remove amniotic epithelial cells so as to obtain the amnion-based biological material. The amnion-based biological material can be used as a mucosal epidermis repair promoting material or epithelial cell repair promoting material so as to promote the tissue reconstruction of the ocular surface, the oral cavity, the respiratory tract, the stomach and the vaginal mucosal. The implementation process is safe, reliable and easy. The source of amnion is wide, and the de-epithelialized amnion can be conveniently prepared. The amnion transplant surgery is simple to operate and easy to implement.

The invention relates to a Chinese yam rice and a preparation method thereof. Rice and Chinese yam are adopted as the raw materials to be processed into Chinese yam rice by twin-screw extrusion and tissue reconstruction. The technical key points lie in that the Chinese yam rice comprises, by mass, 5.0-99.9 parts of rice and 0.1-50.0 parts of Chinese yam powder. The preparation method includes: subjecting the raw materials to crushing, sieving, mixing and conditioning, then carrying out extrusion, granulation and drying, and performing packaging to obtain the finished product. The Chinese yam rice prepared by the method not only has rich nutrition and the functions of invigorating spleen and harmonizing stomach, strengthening physical fitness, and enhancing the body immunity, but also has the cooking characteristics of high quality natural rice. The cooked rice has good texture, and the chewiness and viscoelasticity conform to the consumption preferences in rice diet.

[Problems] A purpose of the invention is to provide highly porous 3D scaffolds made of biodegradable polymer such as poly-lactic acid, which can be preferably used for cell culture.Another purpose of this invention is to provide a method for seeding cells homogeneously inside the above mentioned 3D highly porous scaffolds.Another purpose of this invention is to provide a top-down processing method for obtaining planar porous 2D scaffolds starting the above mentioned highly porous scaffolds to be used as building blocks for thick tissue reconstruction.[Solution] Highly porous scaffolds made of biodegradable polymer such as poly-lactic acid can be fabricated by following steps:(i) biodegradable polymer is dissolved in dioxane at the defined concentration;(ii) a vessel containing the solution is mounted on a metal plate thermally driven by a cold finger to a defined temperature below 10° C., and then the vessel is kept on the plate, until the solution undergoes complete phase separation; and(iii) the obtained solids are immersed into an alcoholic aqueous solution to leach out the dioxane.

The invention discloses a placenta tissue matrix material. A placenta extracellular matrix is prepared by taking placenta tissue as a raw material through the technologies of tissue preservation, tissue treatment, sterilization, virus inactivation and the like, the placenta extracellular matrix retains a natural matrix structure and basic biological characteristics, and the extracellular matrix particularly retains receptors of various active factors. A preparation method comprises the following steps: tissue pretreatment; sterilization and virus inactivation; tissue DNA removal; decellularization; and preparation of various forms such as gel, sponge or powder from the acellular matrix according to specific clinical requirements. The acellular matrix disclosed by the invention is a specialscaffold material with an active factor receptor, can actively attract active ingredients in a living body into the material, is beneficial to accumulation of cell active ingredients, supports cell migration, adhesion, proliferation and differentiation, and can accelerate damaged tissue repair and local tissue reconstruction.