The invention relates to the technical field of traditional Chinese medicine preparations, in particular to an oral skin regeneration promoting oil for treating oral cavity burn and scald and a preparation method and application thereof. The oral skin regeneration promoting oil is prepared from, by weight, 10-30 parts of scutellaria baicalensis, 10-30 parts of coptis chinensis, 10-30 parts of amurcork tree bark, 10-30 parts of Chinese rhubarb, 10-30 parts of astragali radix, 10-30 parts of angelica sinensis, 10-30 parts of garden burnet, 10-30 parts of rehmannia glutinosa, 10-30 parts of angelica dahurica,10-30 parts of blackend swallowwort root, 10-30 parts of bletilla striata, 10-30 parts of frankincense, 10-30 parts of myrrh, 3-8 parts of borneol and 1000-1500 parts of edible vegetableoil, wherein the skin regeneration promoting oil is prepared by heating and mixing the materials in batches. Staining results show that the inflammatory cell infiltration of wound tissues of oral cavity burn and scald site of a rat group using the skin regeneration promoting oil is obviously reduced, the tissue structure is relatively complete, the skin regeneration promoting oil has the effectsof treating oral cavity burn and scald caused by boiling water, acid and alkali. The oral toxicity experiment of the skin regeneration promoting oil show that there is no significant difference in body weight, liver and kidney function of rats compared with a blank group, and no obvious influence is produced.