39 results about "Immunoglobulin IgG" patented technology

The invention discloses a health food for improving immunity and a preparation method thereof. The health food is prepared from the following raw materials in part by weight: 1 to 3 parts of dendrobium officinale, 2 to 6 parts of American ginseng and 6 to 18 parts of Chinese wolfberry. Pharmacological experiments prove that: the health food has an obvious effect of promoting an immune system, canobviously improve a phagocytic index of macrophage of immunosuppressed mice and increase coefficients of immune organs in nonspecifc immune experiments, can improve the conversion rate of lymphocytes, improve the level of immunoglobulin IgG, IgA and IgM in serum of the immunosuppressed mice and assist in conditioning immunodeficiency disease, and diseases such as weakened immune system, low resistance and the like.

The invention relates to a screening method for a hybridoma cell line able to secrete an anti-bovine immunoglobulin IgG monoclonal antibody for bovine immunoglobulin IgG detection, the hybridoma cell line, the monoclonal antibody secreted thereby and application in the field of bovine immunoglobulin IgG detection. The hybridoma cell is preserved in China Center For Type Culture Collection, Wuhan University at Luojiashan, Wuchang, Wuhan City in Hubei Province, and the preservation number is CCTCC No:2013183. The anti-bovine immunoglobulin IgG monoclonal antibody secreted by the hybridoma cell has the advantages of strong specificity, large affinity and high titer and the like, and can be widely applied in the detection reagent or detection equipment field of bovine immunoglobulin IgG. A bovine immunoglobulin IgG rapid detection card built based on the immunochromatography principle can be used for detection of bovine immunoglobulin IgG in colostrum and its products, bovine blood and other samples, and has significant advantages compared with conventional detection methods in the aspects of specificity, sensitivity and detection efficiency, etc.

The invention discloses a fusion protein comprising an Fc domain of IgG and an extracellular domain of an EB virus envelope glycoprotein. The fusion protein is represented by the following formula: P-E-F, wherein P represents a secretion signal peptide, E represents an amino acid sequence of the extracellular domain of the EB virus envelope glycoprotein gp350, and F represents the amino acid sequence of the Fc domain of the IgG. It is found for the first time that after the fusion of the Fc domain of the immunoglobulin IgG with the envelope glycoprotein gp350 from the surface of the EB virus,the immunogenicity in vivo is significantly improved. After immunization with the fusion protein, the total serum titer an immunized animal, the serum specific neutralizing antibody titer and the serum in vitro viral infection blocking efficiency are significantly higher than that of a non-fused control protein. The fusion protein using the Fc domain of the immunoglobulin IgG and the EB virus membrane glycoprotein is adopted as an evidence for the efficacy of an EB virus vaccine and has important practical and theoretical significance and application prospects for prevention and treatment of EB virus-related diseases.

The invention relates to a preparation method of a microcapsule with a function of immune isolation. The method comprises a step of self-assembly through respective layer alternation of a calcium alginate-chitosan microcapsule prepared through complex coaceration and sodium alginate solution as well as chitosan solution. The aperture of the microcapsule is controlled accurately, so that the microcapsule can pass through bovine serum albumin (BSA), but can not pass through immunoglobulin IgG. Therefore, the calcium alginate-chitosan microcapsule with the function of immune isolation can be obtained.

The present invention relates to the field of biomedicine, and specifically provides a human interleukin 10-Fc fusion protein, which is formed by fusing human interleukin 10 with an Fc fragment of immunoglobulin IgG through a connecting peptide; wherein, The Fc fragment is selected from one of human IgG1, IgG2 or IgG4; the amino acid sequence of the human interleukin 10 is shown in SEQ ID No:1. The present invention is formed by fusion of human IL-10 and human immunoglobulin Fc fragment through a specific connecting peptide, which prolongs the half-life of human IL-10 in vivo and increases its stability in vivo. In addition, the present invention provides The human interleukin 10-Fc fusion protein can be used for the treatment of immune diseases and cancer.

The invention discloses a silicon-based rhodamine fluorescent dyeing reagent as well as its preparation method and application, belonging to the fields of organic chemistry and biochemistry. The present invention uses the rhodamine derivatives substituted with silicon atoms as the basic skeleton, and synthesizes a silicon-based rhodamine fluorescent substance with a large Stokes shift and high fluorescence intensity capable of labeling immunoglobulin IgG by modifying different forms of aromatic amines. The staining reagent can be used for in vitro SARS-CoV-2 virus-specific antibody fluorescent ELISA detection. The silicon-based rhodamine fluorescent staining reagent of the present invention has a large Stokes shift (>140nm), can effectively avoid the mutual interference of excitation and emission light, and has high detection sensitivity, and the fluorescent ELISA detection method established based on the fluorescent antibody can be applied Microplate readers with different bandwidths have a wide range of applications.

The invention relates to a method for separating immune globulin IgG from human serum. The method comprises the following steps: 1) diluting the human serum by a buffer solution, adjusting a pH to 6.0to 8.0 and filtering by a filter membrane to obtain a human serum sample; 2) feeding the human serum sample into a chromatographic column, washing by a balancing buffer solution, then eluting by a eluting buffer solution and collecting eluent to obtain the immune globulin IgG solution, the chromatographic column is filled with a combined type bionic chromatography medium, the combined type bionicchromatography medium is prepared from a chromatography matrix and combined type ligand, the chromatography matrix is a hydrophilic porous microsphere with hydroxyl, and a sequence of the combined type ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole; 3) performing utlrafiltration and concentration on the immune globulin IgG solution to obtain the immune globulin IgG. The method can improve an efficiency and a purity for separating the immune globulin IgG from blood.

The invention provides a method for detecting immunoglobulin IgG based on a monodisperse polymer and application thereof. The method comprises the following steps: (1) transferring a sample to be detected into a solid-phase extraction column, performing leaching after affinity chromatography solid-phase extraction, and performing eluting to obtain an eluent, wherein filler of the solid-phase extraction column comprises monodisperse polymer microspheres and protein G connected to the monodisperse polymer microspheres through covalent bonds; and (2) analyzing the eluent by using high performanceliquid volume exclusion chromatography to obtain the content of immunoglobulin IgG in the sample to be detected. According to the method, monodisperse polymer microspheres are used as a binding matrix of protein G, IgG of various subtypes can be captured, other proteins in a sample are not captured, the sensitivity and specificity are good, quantitative analysis is carried out by adopting high performance liquid volume exclusion chromatography, and an obtained chromatogram is good in peak pattern, free of bifurcation and trailing and high in sensitivity and specificity. The method is suitablefor analysis of complex matrix samples such as food and health care products.

The invention relates to a screening method for a hybridoma cell line able to secrete an anti-bovine immunoglobulin IgG monoclonal antibody for bovine immunoglobulin IgG detection, the hybridoma cell line, the monoclonal antibody secreted thereby and application in the field of bovine immunoglobulin IgG detection. The hybridoma cell is preserved in China Center For Type Culture Collection, Wuhan University at Luojiashan, Wuchang, Wuhan City in Hubei Province, and the preservation number is CCTCC No:2013183. The anti-bovine immunoglobulin IgG monoclonal antibody secreted by the hybridoma cell has the advantages of strong specificity, large affinity and high titer and the like, and can be widely applied in the detection reagent or detection equipment field of bovine immunoglobulin IgG. A bovine immunoglobulin IgG rapid detection card built based on the immunochromatography principle can be used for detection of bovine immunoglobulin IgG in colostrum and its products, bovine blood and other samples, and has significant advantages compared with conventional detection methods in the aspects of specificity, sensitivity and detection efficiency, etc.

The invention discloses a staphylococcus protein A, a purification preparation method and application thereof. The invention mainly relates to the construction of a recombinant plasmid by linking a genetically engineered protein ProteinA gene into an expression vector, and then introducing it into a prokaryotic host cell for expression. And and ion exchange chromatography purification, can obtain the target protein with a purity of more than 95%. The purification preparation method of the invention has the characteristics of simple purification process and high yield. At the same time, the prepared ProteinA has good immunoglobulin IgG binding ability, alkali resistance and thermal stability. In addition, the affinity medium prepared by the solid-phase carrier coupled with ProteinA has the advantages of good IgG adsorption effect, high sample loading capacity and alkali resistance. The ProteinA protein prepared by the invention and the medium prepared by coupling can play an important role in the field of monoclonal antibody purification and immunodiagnosis research.

The invention provides a bacillus hainei LY‑23, a bacterial agent, its application and products using the same, and relates to the field of microorganisms. The Bacillus hainei LY-23 is isolated from the large intestine content of a healthy adult black donkey, can utilize a variety of carbohydrates, and has good enzyme activity, among which protease activity, amylase activity and cellulase activity are all better, especially It has extremely strong cellulase activity; it can also improve the animal's digestibility of nutrients in the feed, and increase the content of animal serum immunoglobulin IgG. Based on the function of Bacillus hainenii LY‑23, it can be applied to fermentation, preparation of feed additives, feed, products for improving the intestinal environment of equine animals and products for improving the immunity of equine animals.

The invention discloses an EHV-1 serum antibody detection kit as well as a preparation method and application. The EHV-1 serum antibody detection kit takes a plastic small box as a shell, and a kit inner core is composed of a prefabricated NC film and filter paper; the kit shell is of a cuboid structure; the NC film is coated with purified EHV-1 gD inclusion body protein and pig immunoglobulin IgG respectively; the purified EHV-1 gD inclusion body protein is used as a detection antigen; the pig immunoglobulin IgG is used as quality inspection protein; the purified EHV-1 gD inclusion body protein and the pig immunoglobulin IgG are assembled according to a sequence of a shell cover, the NC film, the filter paper and a shell bottom; a gold label SPA is used as a second antibody, PBS is used as a coating solution, and PBS is used as washing liquid; and an EHV-1 antibody condition in a horse blood serum sample is detected by adopting an antigen and antibody specific combining principle. The method is rapid, visible and simple to operate, is suitable for basic staff to use and has a wide application value.