34 results about "Phenylalanine+Tyrosine" patented technology

The invention provides a hydroxyl phenylalanine tyrosine urine detecting kit, and the detecting kit is at least mixed from an acidic solution, phospho molybdic acid and/or a soluble salt of phosphomolybdic acid; the acidic solution at least contains H+, Ni2+, Hg+ and NO3-, and the concentration of phosphomolybdic acid and/or the soluble salt of phosphomolybdic acid in the detecting kit is 45-900 mmol/L. The detecting kit has a higher detection accuracy.

The invention discloses a method and device of separating and extracting amino acid products from protein hydrolysates. The method comprises the following steps that amino acid mixed solutions are attenuated by water, flow through an I-shaped column, and are washed, ethanol hydrochloric acid mixed solutions flow through the chromatographic column, collecting fluids are obtained by the combination of washing liquor and the ethanol hydrochloric acid mixed solutions, the collecting fluid is adjusted at the PH of about 5.0 to about 6.0, tyrosine can be acquired through filtration, a filter liquor is neutralized by alkali at the PH of more than 7.0, the filter liquor flows through a III-shaped column, and phenylalanine, tyrosine and/or tryptophan are acquired through separation; the ethanol hydrochloric acid mixed solutions flowed through the I-shaped column is adjusted at the PH of more than 7.0, the ethanol hydrochloric acid mixed solutions flow through a II-shaped column, and light components separation solutions, pulp components separation solutions and propanal components separation solutions can be acquired by the sequential adoption of washing, hydrochloric acid extrication and collecting the scrubbing solution in segments; A subsequent separation is conducted for the separation solutions and all the amino acid products are acquired. The method can increase variety of species and yield rate of a single amino acid, the water body for separation and extraction can be recycled, and waste water meets the requirements of environmental emissions.

The invention discloses maltose substrate specificity improved cyclodextrin glycosyltransferase and a preparation method thereof, and belongs to the field of genetic engineering and enzyme engineering. The 47th lysine (Lys) of CGTase of P.macerans strain HFB05-01 (CTCC NO:M208063) is respectively replaced by phenylalanine (Phe), tyrosine (Tyr) and proline (Pro), so that the output of AA-2G is respectively improved by 17.1%, 22.1% and 32.9%. Compared with the wild CGTase, the mutant strains are more conductive to producing AA-2G for glycosyl donors by utilizing maltose, so that important industrial application prospect is provided.

The invention belongs to the field of radiotherapeutic sensitization drugs and particularly discloses novel use of a cathepsin L inhibitor in the preparation of a radiotherapeutic sensitization drug. The invention firstly proves that the activity of cathepsin L is related to the radioresistant mechanism of glioma stem cells and is an important regulating factor in the mechanism for restoring and periodically regulating the damage to the DNA of the glioma stem cells caused by radiation, and the cathepsin L inhibitor can effectively overcome the phenomenon of radioresistance of tumor cells, so that an experimental basis is provided for proving that the cathepsin L is a new acting target position in the radiotherapy of glioma. The invention simultaneously provides the novel use of the cathepsin L inhibitor in the preparation of the radiotherapeutic sensitization drug, wherein the cathepsin L inhibitor is selected from Z-phenylalanine-tyrosine tert-butyl-nitrous ketone or cathepsin siRNA.

The invention relates to a combined ligand, a combined bionic chromatography medium and a preparation method and application thereof. The combined bionic chromatography medium uses hydrophilic porousmicrospheres as chromatography matrix, is activated by allyl bromide, is brominated and alcoholized by N-bromosuccinimide, and then is coupled with the combined ligand. The sequence of the combined ligand is phenylalanine-tyrosine-glutamic acid-5-aminobenzimidazole. The combined bionic chromatography medium has two functional groups including phenylalanine-tyrosine-glutamic acid tripeptide and aminobenzimidazole, introduces a hydrophobicity charge-inducing ligand while retaining the high selectivity characteristic of a polypeptide ligand to an antibody, makes elution conditions milder, and achieves effective antibody isolation.

The invention discloses a tetrapeptide chromatography medium taking phenylalanine-tyrosine-histidine-glutamic acid as a functional ligand, and application of the tetrapeptide chromatography medium; the tetrapeptide chromatography medium can be used for antibody separation. The tetrapeptide chromatography medium is obtained by taking hydrophilic porous microspheres as chromatography matrix, adopting allyl bromide activation and bromo-alcoholization, linking hexamethylenediamine to form a space arm, and then coupling phenylalanine-tyrosine-histidine-glutamic acid as the tetrapeptide ligand. Thetetrapeptide chromatography medium provided by the invention has good binding ability and absorption selection property to an antibody, can absorb the antibody under the neutral condition and efficiently dissociate the antibody under the weak acidic condition, is mild in separation conditions, and can be applied to separation of monoclonal antibodies from cell culture supernatant and separation ofimmunoglobulins from human serum.

The invention relates to a method for separating immune globulin IgG from human serum. The method comprises the following steps: 1) diluting the human serum by a buffer solution, adjusting a pH to 6.0to 8.0 and filtering by a filter membrane to obtain a human serum sample; 2) feeding the human serum sample into a chromatographic column, washing by a balancing buffer solution, then eluting by a eluting buffer solution and collecting eluent to obtain the immune globulin IgG solution, the chromatographic column is filled with a combined type bionic chromatography medium, the combined type bionicchromatography medium is prepared from a chromatography matrix and combined type ligand, the chromatography matrix is a hydrophilic porous microsphere with hydroxyl, and a sequence of the combined type ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole; 3) performing utlrafiltration and concentration on the immune globulin IgG solution to obtain the immune globulin IgG. The method can improve an efficiency and a purity for separating the immune globulin IgG from blood.

The invention relates to a hydrothorax microparticle metabolite combination, a kit and a method for diagnosing tuberculous pleurisy. The pleural effusion microparticle metabolite composition for diagnosing tuberculous pleurisy comprises phenylalanine, tyrosine, methionine, kynurenine, lauroyl carnitine, glycine-binding chenodeoxycholic acid, phosphatidylcholine with the ratio of 36: 7 and lysophosphatidylcholine with the ratio of 20: 2. The kit for diagnosing tuberculous pleurisy comprises an internal standard substance for detecting the concentration of metabolites in the pleural effusion microparticle sample extraction solution. A combined marker provided by the invention has the characteristics of high sensitivity and high specificity for the diagnosis of tuberculous pleurisy, at the same time, can be complementary with a traditional marker adenosine deaminase, and can be used for the auxiliary diagnosis of the tuberculous pleurisy by being combined with the traditional marker adenosine deaminase.

The invention provides a method for extracting low-sodium plant salt contained in low-nitrite sea asparagus in a saline land. The method comprises the steps of planting the sea asparagus in the saline land and extracting the plant salt, wherein the step of planting the sea asparagus in the saline land include seed processing and seedling raising, and the plant salt extracting step include subcritical carbon dioxide-liquid ammonia extraction. By adopting the method for extracting the low-sodium plant salt contained in the low-nitrite sea asparagus in the saline land, the planting yield is 3650-3700 kilograms/mu, and the carbon sequestration capacity of absorbing carbon dioxide of plants per hectare is 5.3-5.5 tons. The nitrite content contained in plant bodies is remarkably reduced, and the yield of the obtained plant salt is improved. The sodium chloride content of the obtained plant salt is 38.1-38.9%, and the obtained plant salt is rich of amino acid, proline, phenylalanine and tyrosine.

The invention belongs to a water-soluble fertilizer suitable for topdressing of apples in a coloring period and an application thereof. The fertilizer is prepared from the following raw materials: urea, industrial monoammonium, monopotassium phosphate, potassium nitrate, urea phosphate, phenylalanine, tyrosine, urine black acid, abscisic acid, gibberellin, jasmonate, ethylene, cane sugar, sodium nitroprusside and genistein. As a water-soluble topdressing fertilizer, the water-soluble fertilizer disclosed by the invention can realize topdressing in the coloring period of apples so as to realize rapid coloring of the apples, also considers the solubility of each component in the fertilizer, and can avoid the influence on the effect of the water-soluble fertilizer due to precipitation generated by interaction of the raw materials; the product has the advantages that after the bags outside the apples are picked off, leaf picking, fruit turning, flower thinning and film laying are not needed, the apples can be rapidly colored, and the fruits have both the appearance and the quality.

The invention discloses a bamboo leaf extract and a preparation method and application thereof, and relates to the technical field of natural product extraction. The bamboo leaf extract comprises a primary bamboo leaf extract obtained by primary extraction with bamboo leaves as raw materials, and a secondary bamboo leaf extract obtained by carrying out secondary processing extraction on primary extraction residues serving as raw materials. The bamboo leaf extract comprises the following raw materials: bamboo leaves, compound enzyme, amino acid, reducing sugar and auxiliaries. The compound enzyme comprises one or more of acidic cellulase, pectinase, beta-glucanase, acidic xylanase, beta-glucosidase and neutral protease, and the amino acid comprises one or more of phenylalanine, tyrosine and proline. According to the bamboo leaf extract and the preparation method and application thereof, secondary processing extraction is carried out on residues obtained after primary extraction of bamboo leaves, resources are fully utilized, and meanwhile the quality of the extract is improved to a certain degree.

The invention discloses a method for endowing collagen with a lasting antibacterial function by using supramolecular chemistry. The method comprises the following steps of firstly preparing cucurbit [7] uril of which the outer wall contains a plurality of short-chain quaternary ammonium groups, then introducing quaternary ammonium groups into the surface of collagen by utilizing the supramolecular inclusion effect of the cucurbit [7] uril on phenylalanine and tyrosine residues, and controlling the number of the quaternary ammonium groups covalently grafted on the outer wall of each cucurbit [7] uril and the length of a covalent connecting arm, and on the premise of not destroying the three-strand helical structure of the natural collagen, enadowing the natural collage with the long-acting antibacterial function.