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A method and equipment for separating and extracting amino acid products from protein hydrolyzate

A protein and amino acid technology, applied in chemical instruments and methods, preparation of organic compounds, preparation of thioethers, etc., can solve the problem of no separation and extraction, and achieve the effect of expanding the degree of separation, high yield and good separation effect

Active Publication Date: 2016-03-30
广州博采生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the amino acid molecular composition of any protein, aromatic amino acid substances (phenylalanine, tyrosine, tryptophan) occupy a considerable proportion, but in this patent, there is no separation and extraction of aromatic amino acid substances

Method used

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  • A method and equipment for separating and extracting amino acid products from protein hydrolyzate
  • A method and equipment for separating and extracting amino acid products from protein hydrolyzate
  • A method and equipment for separating and extracting amino acid products from protein hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Put 100 grams of pig blood meal protein into an 800mL round bottom flask, add 300mL of 6mol / L concentrated hydrochloric acid, heat to above 110°C, hydrolyze for 20 hours, collect the protein hydrolyzate, dilute it with water, let it flow through a 10cm diameter , 15cm high, type I activated carbon adsorption chromatographic column with granular activated carbon body inside, and then wash the chromatographic column with clean water, and use 0.1mol / L hydrochloric acid to prepare a mixed solution of ethanol hydrochloric acid with 50% ethanol concentration to flow through the chromatographic column. After layering the column, wash the chromatographic column with water, combine the water washing liquid and the washing liquid after washing with the ethanol hydrochloric acid mixed solution to obtain the collected liquid, and recover the ethanol by distillation to obtain 150mL protein concentrate and 128mL ethanol solution with a volume percentage of less than 30%. , then use 2m...

Embodiment 2

[0036]Put 100 grams of duck feathers in an 800mL round bottom flask, add 200mL of 10mol / L concentrated hydrochloric acid, heat to above 110°C, hydrolyze for 7 hours, collect the protein hydrolyzate, dilute with water, let it flow through a 10cm in diameter, high 15cm, type I activated carbon adsorption chromatographic column with granular activated carbon body inside, and then wash the chromatographic column with water, and use 0.1mol / L hydrochloric acid to make a mixed solution of ethanol and hydrochloric acid with 50% ethanol concentration to flow through the chromatographic column Finally, wash the chromatographic column with clear water, combine the washing liquid and the washing liquid after washing with the ethanol hydrochloric acid mixed solution to obtain the collected liquid, after distilling and recovering the ethanol, obtain 150mL protein concentrate, and then adjust the protein concentration with 2mol / L ammonia solution The pH value of liquid reaches 5.6, and static...

Embodiment 3

[0039] Put 100 grams of unpeeled rapeseed cake in an 800mL round bottom flask, add 300mL of water to soak for 6 hours, and grind it into a paste, collect the rapeseed cake slurry, adjust the pH value to 7.0, and add 500 units / mL of bacteria Protease was hydrolyzed at 40°C for 3 hours, heated to 100°C and kept for 0.5 hours to inactivate the enzyme, adjusted the pH to 5.0, added 400 units / mL mycoprotease, hydrolyzed at 32°C for 3 hours, raised to 100°C and maintained for 0.5 hours to inactivate the enzyme; Then add 50 units / mL papain and hydrolyze at 31°C for 3 hours, raise the temperature to 100°C and keep it for 0.5 hours to inactivate the enzyme; then add 400 units / mL mycoprotease and hydrolyze at 32°C for 3 hours, filter with Buchner funnel to completely inactivate the enzyme , to obtain the amino acid mixed solution generated by enzymatic hydrolysis; adjust the pH value to 2.5 with 0.1mol / L hydrochloric acid, let it flow through a type I activated carbon adsorption chromato...

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Abstract

The invention discloses a method and equipment for separating and extracting amino acid products from protein hydrolyzate, comprising the following steps: diluting the amino acid mixed solution with water, making it flow through a type I column, washing with water, and then flowing the amino acid mixed solution with ethanol hydrochloric acid After passing through the chromatographic column, combine the washing liquid and the mixed solution of ethanol hydrochloric acid and adjust the collected liquid to a pH value of 5.0~6.0, filter to obtain tyrosine, then neutralize the filtrate with alkali until the pH value is greater than 7.0, and pass it through Type III column to separate phenylalanine, tyrosine and / or tryptophan; adjust the pH value of the mixed solution of amino acids flowing through the type I column to be greater than 7.0, make it flow through the type II column, and then adopt Washing with water, freeing with hydrochloric acid, collecting the washing liquid in sections to obtain the separation solution of bright component, preserved component and C component; and then performing subsequent separation on these separation solutions to obtain all amino acid products. This method can increase the number of single amino acid varieties and yield The water body in the separation and extraction can be recycled, and the waste water meets the environmental protection discharge requirements.

Description

technical field [0001] The invention relates to a method and equipment for separating and extracting amino acid products from protein hydrolyzate. Background technique [0002] At present, the methods for preparing amino acid products are mainly hydrolyzed protein method and microbial fermentation method. These two methods for preparing amino acid products are all carried out in a large amount of water. Wastewater containing amino acid products will seriously pollute the surrounding environment during the discharge process, failing to meet the requirements of environmental protection discharge. [0003] In addition, because the physicochemical properties of various amino acid products are similar or even equal, they will interfere with each other during the separation and extraction process, hindering the process of separating and extracting all amino acid products. It is also for this reason that a large number of animals incidentally produce protein resources such as hair...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C323/58C07C319/28C07C319/20C07C319/22C07C279/14C07C277/08C07D233/64C07C229/08C07C227/40C07C227/18C07D207/16C07C229/26C07C229/24C07C229/22C07C229/36C12P13/22C12P13/06C12P13/12C12P13/24C12P13/10C12P13/14C12P13/20C12P13/08C12P13/04
Inventor 邹学满邹凌邹远飞
Owner 广州博采生物科技有限公司
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