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Hericium erinaceus mycelium and culture method thereof

A Hericium erinaceus and leucine technology, applied in the field of microbial engineering, can solve the problems of difficulty in large-scale production, stable industrial quality, and easy contamination of miscellaneous bacteria, so as to achieve easy bacterial infection, promote anti-tumor, and ensure safety. sexual effect

Active Publication Date: 2014-12-10
HUNAN XINHUI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, due to the problem that traditional solid fermentation is easy to contaminate miscellaneous bacteria, traditional solid fermentation is difficult to produce on a large scale, industrialized, and with stable quality. These are technical problems that need to be solved

Method used

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  • Hericium erinaceus mycelium and culture method thereof
  • Hericium erinaceus mycelium and culture method thereof
  • Hericium erinaceus mycelium and culture method thereof

Examples

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Embodiment 1

[0025] (1) Cultivation of Hericium erinaceus mycelium

[0026] (1) Mother strain culture: prepare the mother culture medium, the ingredients are 400 grams of potato, 42 grams of kana gum, 30 grams of glucose, 10 grams of yeast extract, 10 grams of peptone, 2 grams of potassium dihydrogen phosphate, dihydrogen phosphate Potassium 1g, magnesium sulfate 1g, vitamin B 1 80 mg, Vitamin B 2 40 mg, boil the mother culture medium, put it into a test tube, inoculate Hericium erinaceus strains into the mother culture medium, place it in an incubator for cultivation, and cultivate it at 25°C for 10 days until the slant of the test tube is full of bacteria , that is, the mother strain. The Hericium erinaceus strains used were purchased from Fujian Sanming Fungi Research Institute.

[0027] (2) Original seed culture: Prepare the original seed medium, the ingredients are 70 grams of cottonseed hulls, 20 grams of wheat bran, and 10 grams of corn flour, mix well, bottle, plug the bottle mo...

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Abstract

The invention discloses hericium erinaceus mycelium and a culture method thereof. The hericium erinaceus mycelium contains amino acids and vitamins of relatively high content as well as seven cyclic dipeptides, ergosterol and carboxymethylcellulose activities, wherein the seven cyclic dipeptides are respectively cyc(valine-tyrosine), cyc(leucine-tyrosine), cyc(phenylalanine-tyrosine), cyc(phenylalanine-phenylalanine), cyc(leucine-leucine), cyc(leucine-alanine) and cyc(valine-alanine). The culture method comprises the following steps: culturing a stock strain, culturing a seed strain, culturing a cultispecie strain, performing primary'orientated' biotransformation and performing secondary'orientated' biotransformation. The hericium erinaceus mycelium which is cultured by using the culture method is relatively good in anti-tumor curative effect.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to Hericium erinaceus mycelia containing seven kinds of cyclic dipeptides, ergosterol and carboxymethyl cellulase activity and a cultivation method thereof. Background technique [0002] Hericium erinaceus mycelium is the mycelium obtained by targeted biotransformation of the fungus Hericium erinaceus (Bull.) Pers. It has been used to treat gastrointestinal diseases for more than 2000 years. A series of small Molecular compounds such as erinacines are believed to have nerve regeneration and repair damaged nerve tissue through the blood-brain barrier. Hericium erinaceus is also anti-ulcer, anti-inflammatory, anti-microbial, immune regulation, improving liver function, anti-aging, and lowering blood sugar and blood lipids, improve anti-hypoxic ability, increase cardiac output, and improve circulation, etc., medical preparations containing Hericium erinaceus are widely used in Chin...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61P35/00C12R1/645
Inventor 何述金常耀台
Owner HUNAN XINHUI PHARMA
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