The invention discloses a
fluorescence immunochromatographic
assay detection test paper for
kanamycin residues and a preparation method of the
fluorescence immunochromatographic
assay detection test paper. The
fluorescence immunochromatographic
assay detection test paper comprises a sample
cushion, an
antibody binding
cushion, an NC
cellulose membrane and a bibulous paper, wherein the sample
cushion, the
antibody binding cushion, the NC
cellulose membrane and the bibulous paper are sequentially and mutually overlapped through a bottom plate with adhesives from bottom to top; a
coating detection line, namely a T line and a
quality control line, namely a C line are sequentially arranged at one end close to the
antibody fixation cushion, of the NC
cellulose membrane; the antibody fixation cushion is used for bearing conjugates of fluorescence labeling antibodies; the T line which is sprayed and drawn by a
coupling coating antigen Kana-BSA and a C line which is sprayed and drawn by a goat anti-rat secondary antibody IgG are separately arranged on the NC cellulose membrane from top to bottom; a fluorescence
labelling probe is made from
rare earth fluorescence
silicon dioxide nanometer particles, and the fluorescence labeling probe is bound to the antibodies by using
aldehyde group
glucan as a cross-linking
reagent; the preparation conditions of the fluorescence immunochromatographic assay test paper strip are inspected by a gel imaging
system and a fluorescence chart reader; and the
fluorescence intensity of the T line is detected by the fluorescence chart reader, and the quantitative detection is completed through a
standard curve of the
kanamycin antigen concentration.