The invention discloses a binary anti-collision method with a security mechanism in RFID, comprising: a new low-cost security authentication mechanism and a conventional RFID binary anti-collision method. The main contribution of the invention is that the conventional electronic tag binary anti-collision method is vulnerable to various attacks such as eavesdropping and tracking, and the invention enhances the security performance of RFID by introducing a new security authentication mechanism. From the perspective of application, the present invention combines physical methods and cryptographic mechanisms, and proposes a new way to solve the RFID privacy problem, which includes a Hash encryption module, a cycle counting address selection circuit, an anti-malicious test self-destruction module, and a terminal database. The present invention adopts Hash encryption technology and a plurality of pseudonym technologies comprehensively, can be applied in distributed RFID application system, and its main technical characteristic is: do not use Hash encryption module in label, but adopt Hash encryption module in RFID reader, like this More than 20,000 circuits can be saved in the label. In addition, the establishment of multiple pseudonyms can effectively prevent being tracked. The invention not only realizes the encryption function, but also can effectively reduce the cost of the electronic label, and is beneficial to the large-scale promotion of the RFID label in the market.