67 results about "Lymphocyte transformation" patented technology

The invention discloses a health food for improving immunity and a preparation method thereof. The health food is prepared from the following raw materials in part by weight: 1 to 3 parts of dendrobium officinale, 2 to 6 parts of American ginseng and 6 to 18 parts of Chinese wolfberry. Pharmacological experiments prove that: the health food has an obvious effect of promoting an immune system, canobviously improve a phagocytic index of macrophage of immunosuppressed mice and increase coefficients of immune organs in nonspecifc immune experiments, can improve the conversion rate of lymphocytes, improve the level of immunoglobulin IgG, IgA and IgM in serum of the immunosuppressed mice and assist in conditioning immunodeficiency disease, and diseases such as weakened immune system, low resistance and the like.

A Chinese medicine for regulating immunity and suppressing tumor is prepared from pilose asiabell root and astragalus root (1:1). Its preparing process is also disclosed.

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purity forsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

The invention aims to provide lactobacillus rhamnosus having bacteriostatic and immunity enhancement function, with the preservation number: CCTCC NO: M 2013355. The lactobacillus rhamnosus provided by the invention can be applied in the biological products which are medicines, food or health products. The lactobacillus rhamnosus has very powerful bacteriostatic ability, can obviously improve the achroacyte conversion and HCso of a mouse, phagocytosis rate and index of macrophage and NK cell activity, can enhance normal mouse immunity, and therefore can be widely applied in the medicines, food or the health products.

The invention relates to a prunella spike polysaccharide composite with immunity activity, and relative production, wherein its production comprises that: boiling the dry fruit of Prunella vulgaris L, extracting, depositing with alcohol, filtering the components whose molecule weight is higher than 3000Da, filtering and purifying. The polysaccharide content is higher than 50%, the polysaccharide molecule is higher than 5000Da. And it is formed by A, B-1, B-2, C, and D. The invention can improve lymphocyte transfer gain and induce IFN- gamma, to be used as active component to prepare the drug that improves the immunity of mammal.

The invention discloses a health product for improving bone density and immunity, which active ingredients have the following weight ratios: 60-140 radix puerariae extract, 120-280 D-dextrosamine and 120-280 calcium. The advantages of the health product in the invention are in that: rapid releasing, easy for absorbing, improving arthrosis pain caused by bone loss, preventing and treating various diseases caused by cartilage tissue cataplasia; distinctly increasing ingestion action of liver and spleen macrophage to ingest carbon granules, distinctly accelerating lymphocyte transformation and raising phagocytosis rate of the macrophage to obviously improve nonspecific immunity function of human body. Accordingly, the invention has good and fast effect for increasing bone density and immunity.

The invention relates to a process for producing biscuit food, in particular cakes containing Chinese medicinal herb polysaccharides, calculated by weight ratio, the constituents of the raw material include flour 100-150 parts, salad oil 30-35 parts, sugar powder 20-25 parts, ammonium bicarbonate 1-3 part, edible Chinese medicinal polysaccharides 0.05-0.1 part, the producing process consists of modulating into flour dough with 20-30 parts of water, modeling and baking.

The invention discloses a method for preparing immunoactive peptide by taking red pine seed meal as a raw material, belonging to the technical field of agricultural product deep processing and comprehensive utilization of byproducts thereof. The red pine seed meal immunoactive peptide with molecular weight of 3,000-10,000 Da, protein content of 92-96 percent, moisture of 2-4 percent, 0.5-1.5 percent of ash content, 0.40-0.60 percent of lymphocyte conversion value and 45-65 percent of NK (Natural Killer) cell activity is obtained through raw material pretreatment, complex enzyme controllable enzymolysis, membrane separation, vacuum concentration and vacuum freeze drying processes, and immunoactive evaluation is performed by using lymphocyte conversion and NK cell activity measurement. The selected red pine seed meal is originated from the byproducts produced after oil expression of the red pine seed meal by a cold expression method, and is not in contact with any organic extraction solvent, the red pine seed meal immunoactive peptide is conveniently and effectively separated, and the original quality and functions of the red pine seeds of Changbai mountains are maintained.

The invention relates to an infusion protein of an IDB (Infectious Bursal Disease) virus VP2 protein and an LTB (Leukotrienes B) of enterotoxin of escherichia coli, wherein the amino acid sequence of the infusion protein is SEQ ID NO: 1. According to the invention, the supervirulent VP2 protein of the IBDV (Infectious Bursal Disease Virus) is expressed with the LTB protein of enterotoxin of escherichia coli with a mucosal immunologic adjuvant in a fusion manner, and an expression product is emulsified to prepare a vaccine to make SPF chickens which are 21 days old immune. An immune reference and a non-immune reference for a subunit vaccine of VP2 are set. After 7 days of immunity, the lymphocyte transformation rate is determined, and after 21 days of immunity, blood is collected to determine the valence of antibody of immunodiffusion of serum agar and toxic substances are counteracted together with the non-immune reference. The result shows that vaccine immunity groups can resist virulent attach. The lymphocyte transformation rate and the serum valence of antibody are remarkably higher than the immune reference group of VP2, so that the infusion protein has an excellent immunogenicity.

The invention provides a method for preparing natural antibiotics- recombined chicken- beta Buchner's bodies Gal-9. The invention employs molecular biology technology to clone chicken- beta Buchner's bodies Gal-9 gene from chicken, and expresses chicken- beta Buchner's bodies Gal-9 protein outside chicken body by using proper expression host. It is found by further research that the chicken- beta Buchner's bodies Gal-9 possesses wide anti bacteria activity and immunologic enhancement. The recombined chicken- beta Buchner's bodies Gal-9 is characterized in that: (1) ir can inhibit and kill bacillus coli, salmonella, Micrococcus pyogenes and so on, (2) prevents avian infectious brunchitis virus, (3) increases antibody for avian infectious brunchitis virus concentration by 10- 20 % in dimefox after oral administration for one week by chicken, improves apleen lymphocyte blastogenesis by 40- 50%.

The invention provides a method for preparing natural antibacterial agent- recombination chicken- beta defensins Gal-9-Gal-8 bi-molecule protein. The invention employs molecular biology technology and colones chicken- beta defensins Gal- 8 and Gal-9 gene inside chicken and expresses defensins Gal-9-Gal-8 bi-molecule protein outside chicken body by employing proper expression host. The recombined chicken- beta defensins Gal-9-Gal-8 bi-molecule protein is characterized in that: (1) it can inhibit and kill bacillus coli, salmonella and salmonella and so on, (2) it can resist avian infectious brunchitis and newcastle disease virus, (3) it can increase the antibody for avian infectious brunchitis in dimefox by 10-20%, antibody for newcastle disease virus by 10-20% and increase chicken splenocyte lymphocyte conversion rate by 60-70%.

The invention belongs to the technical field of cell mediums, and in particular relates to a human peripheral blood lymphocyte medium and a preparation method thereof. The medium provided by the invention mainly consists of the following components: an RPMI1640 medium, fetal bovine serum, collagen, sodium hyaluronate, glucagon, thyrotropin releasing hormone, total glucosides of paeony, linoleic acid, butanediamine, gluconic acid iron salt hydrate, glutathione and polylysine. The medium provided by the invention can be used for effectively inhibiting microorganisms and viruses, and the medium is good in reagent stability and high in transformation rate of cultured lymphocytes and lymphocyte division index.

The invention provides a compound feed additive for enhancing broiler chicken immunity. The compound feed additive is prepared by 28-50 parts of rhodiola glucoside, 15-27 parts of hydrochloric acid berbamine, 9-22 parts of pueraria root extract, 7-20 parts of schisanhenol and 6-16 parts of eupolyphaga powder. The compound feed additive can effectively promote growth of broiler chicken immune organs, obviously improve the level of immune globulin IgG in serum, the lymphocyte transformation rate and the leukocyte phagocytic ability, increase the number of T-lymphocytes and enhance the body liquid and the cellular immune function of broiler chickens, and therefore the incidence rate of diseases and the use frequency of antibiotic and other medicine are reduced, and direct economic and social benefits are achieved.

The invention belongs to the technical field of cell culture media, and concretely relates to a human peripheral blood lymphocyte culture medium, which mainly contains the following components and concentration thereof: an RPMI1640 liquid culture medium, Ham's 12, bovine serum, hyaluronic acid 3, casein phosphopeptides, phytagglutinin, lyciumbarbarum polysaccharide, baicalin and nisin. The culture medium provided by the invention can be beneficial for effectively inhibiting actions of microorganisms and viruses, promoting cell proliferation, and shortening cell culture cycle, and has the advantages of high lymphocyte conversion rate and lymphocyte division index, and good reagent stability.

An application of baikal skullcap polysaccharides in preparation of a veterinary drug is disclosed. The baikal skullcap polysaccharides are used for preparing an additive in a veterinary drug enhancing immune functions of animals, and are prepared through extraction of baikal skullcap plants. The concentration of the baikal skullcap polysaccharides in the veterinary drug is 31.25 [mu]g / mL to 4 mg / mL. Pig lymphocyte transformation tests show that the baikal skullcap polysaccharides can promote lymphocyte transformation, can increase contents of the total protein (TP), serum albumin (ALB), serumglobulin (GLOB), lymphocytes and monocytes in serum of rats with low immunity so as to restore body conditions of the rats, thus proving that the baikal skullcap polysaccharides have effect of significantly enhancing animal body immune functions and can be used in a veterinary drug enhancing animal body immune functions.

The invention discloses traditional Chinese medicine liquor for treating tumors and belongs to the field of traditional Chinese medicine. The traditional Chinese medicine liquor mainly comprises more than 60 kinds of traditional Chinese medicines including cortex juglandis mandshuricae, fructus fici, livistona chinensis (Jacq.) R.Br., trapa natans L. (quadrangular), rabdosia rubescens, spreading hedyotis herb, rhizoma smilacis glabrae, barbed skullcap herb, Indian mockstrawberrg herb and the like, and is prepared by soaking the traditional Chinese medicines in pure-grain Baijiu (55-60% vol) according to a specific ratio, a specific sequence and specific time and then filtering. The traditional Chinese medicine liquor has the advantages that the traditional Chinese medicine liquor is used for treating various tumors (cancers) and applied to other metastatic cancer treatment and is capable of strengthening the body resistance, removing evils, softening hardness to dissipate stagnation, resisting cancers, detoxifying, reinforcing the vital essence, strengthening the primordial qi, activating blood circulation to remove stagnated blood, nourishing blood, achieving hemogenesis, invigorating the stomach, tonifying the spleen, clearing blood, cooling blood and clearing heat-fire; phagocytic ability of macrophagocytes can be improved, cancer cells can be killed directly or indirectly to promote lymphocyte transformation and induce interferon, canceration inducing factors are decreased, and accordingly cancer prevention, resistance to the cancers, cancer inhibition and cancer cell apoptosis are achieved; various cancers and forming thereof as well as a series of diseases caused by the various cancers can be prevented.

The invention discloses compound astragalus polysaccharide injection used for pigs and a preparation method thereof. Every 100L of compound astragalus polysaccharide injection used for the pigs contains the following raw materials, such as 0.5-5kg of astragalus polysaccharide, 1-10kg of lincomycin, 5-30kg of analginum, 1-10kg of inosine, 3-6L of 2-hydroxyl ethylamine and the like, wherein the astragalus polysaccharide can accelerate the lymphocyte to be converted and increased in value and accelerate the phagocytic function of macrophage; the lincomycin has strong antibiosis efficacy; the astragalus polysaccharide and the lincomycin are organically combined to perform an antiviral function, widen an antibacterial spectrum and enhance a targeted action on bacteria; the analginum has various functions of resisting bacterium, diminishing inflammation, carrying out antipyretic action, relieving pain and the like; and the inosine is favorable for restoring the function of a damaged hepaticcell. Above medicines have a reasonable formula, can simultaneously carry out symptomatic treatment and etiological treatment by aiming at diseases, has quick curative effect on mixed infection of pig virus and bacteria and treats both principal and secondary aspect of the disease, the medicine resistance of the pathogenic microorganism is lowered, the clinic symptom of a diseased pig is alleviated, and therefore the pig is quickly restored.

The invention discloses a medium freeze-drying powder production process for lymphocyte culture, which comprises following steps: (1) preparing medium stock solution; (2) filtering medium stock solution, freezing, drying and obtaining the freeze-drying powder; (3) adding mixing gas containing nitrogen and oxygen into the freeze-drying powder, and obtaining the product. According to the process provided by the invention, a mixing gas containing nitrogen and oxygen at a certain ratio is added in the freeze-drying stage after the secondary drying ends, thereby activity and stability of the medium freeze-drying powder are unexpectedly improved. After a period of time,the freeze-drying powder compound solution is used for blood lymphocyte culture and chromosome karyotype analysis, and the two key active indexes such as lymphocyte conversion rate and mitotic phase index remain stable and are substantially higher than those of the traditional liquid medium. The lymphocyte medium freeze-drying powder prepared by the process provided by the invention has the advantages of long preservation time, stable effect, convenient storage, carrying and transport, thereby the commercialization of the lymphocyte medium is realized.

The invention discloses an application of a rodgersia sambucifolia extract in preparation of a veterinary drug. The rodgersia sambucifolia extract is used for preparing a drug additive of the veterinary drug for enhancing the immune function of animal bodies, the rodgersia sambucifolia extract is a crude flavonoid extract extracted from a rodgersia sambucifolia plant, the crude flavonoid extract of rodgersia sambucifolia is applied to the drug additive of the veterinary drug, and the drug concentration in the veterinary drug is 1.25-5 mg / mL. The obtained crude flavonoid extract of rodgersia sambucifolia has good biological activity and can promote transformation of lymphocytes in swine lymphocyte transformation experiments, increase the content of WBCs (white blood cells), Gran (neutrophilicgranulocyte) and Lym (lymphocyte) in serum of rats with low immunogenicity and recover the physical condition of rats with immunosuppression. It is indicated that the crude flavonoid extract of rodgersia sambucifolia has the effect of significantly enhancing the immune function of animal bodies and can be used as a raw material of the veterinary drug for enhancing the immune function of animal bodies.

The invention belongs to the technical field of biological products for animals, and particularly relates to diluent for a live vaccine for hog cholera. 5g to 10g of agaricus blazei polysaccharide, 1g to 5g of herba andrographitis, 0.1g to 2g of ginsenoside Rh2 and 2g to 6g of curcumin are contained in every 1,000ml of a phosphate buffer solution, the pH (potential of Hydrogen) of which is equal to 7, of the diluent. The diluent for the live vaccine for the hog cholera, which is provided by the invention, is not only convenient to produce, low in cost and convenient and safe to use, but also can be used for improving the conversion ratio of a lymphocyte, decreasing a difference among vaccines of different batches and improving the immunization overall effect of the vaccines.

The invention relates to a preparation method of a wood frog spawn polysaccharide extract, and an application of the wood frog spawn polysaccharide extract in immunity enhancement. The method is characterized in that the method comprises the following steps: extracting wood frog spawn polysaccharides from wood frog spawn as a raw material through an enzymatic hydrolysis technology, filtering or centrifuging, carrying out alcohol precipitation on the above obtained filtered or centrifuged solution with anhydrous ethanol 3 times the volume of the filtered or centrifuged solution to obtain a precipitate which is the polysaccharide extract, freeze-drying or oven-drying to prepare an extract or dry powder; and the obtained ethanol liquid is recovered below 40DEG C. The wood frog spawn polysaccharides have mouse lymphocyte conversion promotion effects on in vitro mouse spleens to different degrees, a good dose-effect relationship exists, and the polysaccharide extract with the concentration of 0.001[mu]g / mL still has a strong lymphocyte conversion promotion effect. In mouse in vivo experiments, a wood frog spawn polysaccharide sample has an obvious lymphocyte conversion promotion effect. In the field of the influences on cytokines in mouse serum, the influences of the wood frog spawn polysaccharides on the outputs of TNF-alpha and IL-1beta in the serum substantially change. The wood frog spawn polysaccharide extract can be used to prepare immune medicines, and can also be used as a food additive. The extraction method and the extract solvent are not limited to that mentioned in the experiments and the embodiments.

The invention relates to a method for evaluating Dorking resistance, which comprises the following steps of: measuring the following indexes: lymphocyte conversion rate; the number of monoclonal antibody-labeled CD4+, CD8+ and T cells and a ratio of the CD4+ to the CD8+; IgG and IgM; an immunocyte index, namely absolute weight of thymus, spleen, and bursa of Fabricius, and the chicken body weight; a blood index, namely red blood cell, and total number of white blood cells, T cells and B cells in per milliliter of blood; and a cell factor index, namely interferon at 7 growth stages every two weeks from 0 to 12 weeks; and performing comprehensive evaluation on the six indexes, and using a comprehensive score as a basis for judging the degree of sample resistance. Compared with the prior art, the method for evaluating the Dorking resistance has the advantages of comprehensiveness and accuracy of Dorking resistance evaluation and wide application prospects.

The invention discloses a preparation method of functional yam-flavored wine and belongs to the technical field of preparation of functional wine. The preparation method comprises steps as follows: preparation of a yam saccharification liquid, preparation of glutinous rice saccharification mash, dry yeast activation, mixed fermentation, clarification, ageing and sterilization. The yam-flavored healthcare wine has stable quality, mellow taste and good healthcare effects. Yam has the functions of reducing blood glucose, resisting oxidation, delaying senescence and resisting tumor and mutation and can improve the intestinal function. Yam polysaccharide can enhance the capability of lymphocyte proliferation, has a function of promoting antibody generation and can obviously increase the macrophage phagocytosis percentage and phagocytic index under the condition of low immune function caused by cyclophosphamide, promote formation of mature hemolysin and hemolysis plaque and conversion of lymphocyte and remarkably increase the peripheral T-lymphocyte ratio.

The invention belongs to the technical field of cell culture mediums, and discloses a human peripheral blood lymphocyte culture medium. The human peripheral blood lymphocyte culture medium mainly comprises the following components: RPMI 1640 culture medium powder, sodium bicarbonate, HEPES free acid, gentamycin sulphate, heparin sodium, mercaptoethanol, glutamine, calcium ions, iron ions, titaniumions and phytohemagglutinin. The human peripheral blood lymphocyte culture medium can contain or be free of serum or protein components, and the culture medium mainly uses certain specific metal ionsto promote cell proliferation. According to the culture medium, the composition is clear, the performance is stable, the cost is low, proliferation and division of lymphoblasts in human whole blood can be effectively promoted, and the high lymphocyte transformation rate and a high lymphocyte division index are achieved.

The invention relates to an anti-tumor traditional Chinese medicinal composition. The anti-tumor traditional Chinese medicinal composition is prepared from the following raw medicinal materials by weight: 1-7 g of radix aconiti kusnezoffii, 1-10 g of radix aconiti lateralis, 1-10 g of rhizoma pinelliae, 1-10 g of rhizoma arisaematis, 1-10 g of thallus laminariae et eckloniae, 1-10 g of borneol, 1-10 g of cortex cinnamomi, 5-25 g of radix glycyrrhizae, 0.5-5 g of calomelas, 2-15 g of centipedes, 2-15 g of spiders, 1-10 g of mylabris, 2-20 g of radix ginseng rubra, 2-15 g of colla cornus cervi,3-15 g of radix notoginseng, 3-15 g of scorpio, and 2-15 g of squama manitis. The anti-tumor traditional Chinese medicinal composition provided by the invention is used for dredging the channels, stimulating the vitality of macrophages, improving the transformation rate of lymphocytes, inhibiting the growth of cancer and tumor cells, damaging the fibrous protein agglutination around the tumor andin the foci, preventing implantation of cancer cells, and preventing the growth and metastasis of tumor.

The invention discloses a composition for enhancing the immunity and protecting gastric mucosa and a use method, and belongs to the field of composition chewable tablets. The composition comprises, byweight, 140-160 parts of hericium erinaceus extracts, 70-90 parts of fructus lycii extracts, 70-90 parts of poria cocos extracts, 70-90 parts of Chinese date extracts, 240-260 parts of starch, 70-90parts of beta- cyclodextrine, 8-9 parts of magnesium stearate, 4-6 parts of silicon dioxide, 5-7 parts of maize germ oil, 240-280 parts of fruit-vegetable powder and 1-2 parts of pseudo-ginseng powder. The mixture A is put in a tabletting machine for tabletting forming, each tablet of composition has the weight of 1 g, and a user takes the composition twice every day and takes two tablets each time by chewing. The composition enhances the phagocytic function of macrophages, promotes the lymphocyte transformation, excites the immune, resists ageing, replenishes blood and protects the liver.

The invention discloses health-care jelly powder for children. The health-care jelly powder for children is characterized by being mainly prepared from the following components: nut powder, carrot powder, seaweed powder or seaweed micro-slices, carrageenan, konjaku flour, sodium citrate, citric acid, potassium chloride and white sugar. In the health-care jelly powder for children, fatty acids contained in the nut powder are generally unsaturated fatty acids which are very helpful to the intelligence development of children, and the nut powder is rich in vitamin D which has the most important physiological function of promoting calcium absorption and utilization of children; the carrot powder has the effects of benefiting the liver and improving eyesight, benefiting the diaphragm and relaxing the intestines, promoting metabolism, enhancing immunity and the like, and the vitamin A in the carrot powder is capable of improving the eyesight of children, preventing night blindness, promoting skeleton growth, enabling teeth to be solid and promoting children to grow; and the seaweed powder or seaweed micro-slices is or are rich in choline, calcium and iron which are capable of enhancing memory, promoting skeleton growth, promoting tooth growth and enabling the teeth to be healthy, and polysaccharides contained in the seaweed powder or seaweed micro-slices have functions of obviously enhancing cell immunity and humoral immunity and are capable of promoting lymphocyte transformation and improving body immunity.

The invention relates to the technical field of medical detection, and particularly relates to a method for detecting lymphocyte transformation by utilizing a medicament cytochalasins B. According to the invention, the principle that peripheral blood lymphocytes can be transformed into lymphoblasts which are divisive after being stimulated by phytohemagglutinin in vitro and the principle that the cytochalasins B can retard cell division but can not retard cell nucleus division are utilized, 2 Gyg ray irradiation is carried out on the peripheral blood of a human body for further realizing immunodepression, and PHA (phytohemagglutinin) stimulation is carried out and the cytochalasins B is added for treatment, so that the difference between the dikaryon rate of radiated lymphocytes and the dikayron rate of non-irradiated lymphocytes is detected. The result shows that transformed lymphocytes can form dikaryocytes, the dikaryon rate of the radiated lymphocytes can be obviously reduced, the immunity of radiated T lymphocytes can be obviously inhibited, and the dikaryon rate can better reflect the immune function of the lymphocytes. The method is intuitive, simple in operation, rapid, economical, and accurate in result during detection of indexes.

The invention discloses a compound chitosan oligosaccharide meal capable of conditioning symptom of cancer and a preparation method of the compound chitosan oligosaccharide meal. The meal comprises the following raw materials in percentage by mass of 2-4% of chitosan oligosaccharide powder, 43%-53% of compound fungus polysaccharide, 30%-40% of a snow lotus herb culture, and 3%-25% of an astragalusextract. The preparation method of the meal comprises the following steps of separately preparing the chitosan oligosaccharide powder, the compound fungus polysaccharide, the snow lotus herb cultureand the astragalus extract, performing weighing in mass ratio, and performing mixing. The raw materials of the meal are not treated by a conventional crushing and boiling method, but are completely treated by a modern traditional Chinese medicine fermentation extraction technique, hazardous substances in the raw materials are degraded, and active components in traditional Chinese medicines are efficiently obtained, and various active polysaccharide is applied. The active polysaccharide can stimulate immunocompetence, can strengthen the effect of reticuloendothelium for phagocytose of tumor cells, can promote lymphocyte transformation, can activate T cells and B cells, and can promote antibody formation, so that the meal has the activity of resisting tumors to a certain extent.