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Method for preparing immunoactive peptide by taking red pine seed meal as raw material

A technology for immune active peptides and Korean pine seeds, which is applied in the field of preparing immune active peptides with Korean pine bean meal as raw material, can solve problems such as unseen natural high-quality Korean pine seed meal, etc., and achieves high product utilization value and technological route. Simple, value-added effects

Active Publication Date: 2014-02-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, existing reports have shown that immunoactive peptides with different biological functions, such as antithrombotic invertase inhibitory peptides, casein phosphopeptides, antibacterial peptides, etc., can stimulate the proliferation of lymphocytes in the body and enhance the phagocytosis of macrophages , but in the existing research, there is no research on immunoactive peptides using natural high-quality Korean pine seed meal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] With protein content of 32%, fat content of 15%, starch content of 26%, water content of 2%, ash content of 7% of the Korean pine seed meal as raw material, the use of deionized water at a temperature of 18 ℃, according to the red The ratio of pine nut meal and water is 5: 100g / mL for mixing, after soaking for 12 hours, place it in an ultrasonic processor, adjust the pH of the Korean pine nut meal soaking liquid to 6.0, set the working parameters of the ultrasonic processor, the power is 100W, the temperature The temperature is 45°C, and the treatment time is 33 minutes. After ultrasonic treatment, the solidified red pine nut oil layer is peeled off to obtain the ultrasonic treatment solution. When the temperature of the feed liquid in the tank is 89°C, after maintaining the temperature for 10 minutes, start the cooler to lower the temperature of the feed liquid in the reaction tank to 35°C, and then use 8.0mol / L sodium hydroxide solution to adjust the feed liquid in the...

Embodiment 2

[0033] With protein content of 35%, fat content of 25%, starch content of 25%, water content of 3%, ash content of 5.5% of the Korean pine seed meal as raw material, the use of deionized water at a temperature of 19 ℃, according to the The ratio of pine nut meal and water is 8: 100g / mL to mix, after soaking for 19h, place in the ultrasonic processor, adjust the pH of the Korean pine nut meal soaking liquid to 7.5, set the working parameters of the ultrasonic processor, the power is 300W, the temperature The temperature is 35°C, and the treatment time is 25 minutes. After the ultrasonic treatment, the solidified red pine nut oil layer is peeled off to obtain the ultrasonic treatment solution. When the temperature of the feed liquid in the tank is 95°C, after maintaining the temperature for 11 minutes, start the cooler to reduce the temperature of the feed liquid in the reaction tank to 45°C, and then use 9.0mol / L sodium hydroxide solution to adjust the feed liquid in the reactio...

Embodiment 3

[0035] With protein content of 40%, fat content of 19%, starch content of 34%, water content of 4%, ash content of 6% of the Korean pine seed meal as raw material, the use of deionized water at a temperature of 20 ℃, according to the red The ratio of pine nut meal and water is 7: 100g / mL to mix, after soaking for 14h, place in the ultrasonic processor, adjust the pH of the Korean pine nut meal soaking liquid to 6.5, set the working parameters of the ultrasonic processor, the power is 250W, the temperature The temperature is 39°C, and the treatment time is 35min. After the ultrasonic treatment, the solidified red pine nut oil layer is peeled off to obtain the ultrasonic treatment solution. When the temperature of the feed liquid in the tank is 85°C, keep the temperature for 14 minutes, start the cooler, reduce the temperature of the feed liquid in the reaction tank to 37°C, and adjust the feed liquid in the reaction tank with 8.5mol / L sodium hydroxide solution The pH of the liq...

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Abstract

The invention discloses a method for preparing immunoactive peptide by taking red pine seed meal as a raw material, belonging to the technical field of agricultural product deep processing and comprehensive utilization of byproducts thereof. The red pine seed meal immunoactive peptide with molecular weight of 3,000-10,000 Da, protein content of 92-96 percent, moisture of 2-4 percent, 0.5-1.5 percent of ash content, 0.40-0.60 percent of lymphocyte conversion value and 45-65 percent of NK (Natural Killer) cell activity is obtained through raw material pretreatment, complex enzyme controllable enzymolysis, membrane separation, vacuum concentration and vacuum freeze drying processes, and immunoactive evaluation is performed by using lymphocyte conversion and NK cell activity measurement. The selected red pine seed meal is originated from the byproducts produced after oil expression of the red pine seed meal by a cold expression method, and is not in contact with any organic extraction solvent, the red pine seed meal immunoactive peptide is conveniently and effectively separated, and the original quality and functions of the red pine seeds of Changbai mountains are maintained.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products and comprehensive utilization of by-products, and relates to a method for preparing immunoactive peptides by using Korean pine seed meal as a raw material. The immunoactive peptide of Korean pine seed meal with water content of 2.0%-4.0%, ash content of 0.5%-1.5%, lymphocyte transformation value of 0.40-0.60, and NK cell activity of 45%-65%. Background technique [0002] Our country has a long history of producing Korean pine nuts, and it is the main exporting country of Korean pine nuts. Changbai Mountain is the main production area of ​​Korean pine nuts. It has unique advantages in Korean pine nut resources. In the pollution-free natural environment, most of them belong to organic or green food, and the quality is also famous for a long time. However, the production of Korean pine nuts in my country is still in the stage of collecting wild resources, and the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 林松毅刘轩廷刘博龙贾亚平王爽刘颢然姜源
Owner JILIN UNIV
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