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Preparation method of wood frog spawn extract with immunity enhancing function

A technology for enhancing immunity and Rana eggs, applied to medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., to achieve the effects of enhancing the body's immunity, regulating the body's immunity, and significantly improving the body's immunity

Inactive Publication Date: 2014-11-12
卫功庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lu Hongyan and others [61] Studies have shown that the enzymatic hydrolyzate of wood frog eggs can improve the symptoms of hyperlipidemia in ovariectomized rats, and can well inhibit liver cell damage. Therefore, wood frog eggs need to be developed in the prevention of cardiovascular and cerebrovascular diseases

Method used

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  • Preparation method of wood frog spawn extract with immunity enhancing function
  • Preparation method of wood frog spawn extract with immunity enhancing function
  • Preparation method of wood frog spawn extract with immunity enhancing function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 The production method of wood frog egg polysaccharide: Accurately weigh dry wood frog egg powder, add a certain amount of distilled water, put it in a shaker, soak it at 100r / min for 2 hours, take it out, and adjust the required pH value. Add the enzyme reagent, put it in the shaker, 100r / min, at 55 。 Enzyme hydrolyze at C, then heat in a boiling water bath for 10min to inactivate the enzyme activity, centrifuge at 5000r / min for 10min, remove the precipitate, take the supernatant, use the Sevage method to remove protein, pour it into a separatory funnel and shake it for 20-30min, statically After the layers were separated, the upper and lower layers were centrifuged at 9000r / min for 15min, and the supernatant was mixed, and 3 times the volume of absolute ethanol was added for alcohol precipitation, and left at low temperature overnight, and centrifuged at 9000r / min the next day. After 15 minutes, the precipitate was taken and dried to obtain the polysaccharide...

Embodiment 2

[0084] Embodiment 2 Rana egg polysaccharide capsule

[0085] Take the polysaccharide powder of Rana ovum of Example 1 (through a sieve of 80-200 mesh), put it into capsules, and each capsule contains a certain proportion of Rana ovum polysaccharide. Other requirements are in line with the relevant regulations on capsules in the Pharmacopoeia of the People's Republic of China (2010 Edition).

Embodiment 3

[0086] Embodiment 3 Rana egg polysaccharide tablet

[0087] Take a certain amount of polysaccharide powder (passed through a 80-200 mesh sieve) of Rana ova eggs in Example 1, 20 g of cyclodextrin, an appropriate amount of dry starch, and an appropriate amount of carboxymethyl cellulose, mix well, granulate and press into tablets, and the obtained tablet weighs 0.50 g. Other requirements are in line with the relevant regulations on tablets in the Pharmacopoeia of the People's Republic of China (2010 Edition).

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PUM

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Abstract

The invention relates to a preparation method of a wood frog spawn polysaccharide extract, and an application of the wood frog spawn polysaccharide extract in immunity enhancement. The method is characterized in that the method comprises the following steps: extracting wood frog spawn polysaccharides from wood frog spawn as a raw material through an enzymatic hydrolysis technology, filtering or centrifuging, carrying out alcohol precipitation on the above obtained filtered or centrifuged solution with anhydrous ethanol 3 times the volume of the filtered or centrifuged solution to obtain a precipitate which is the polysaccharide extract, freeze-drying or oven-drying to prepare an extract or dry powder; and the obtained ethanol liquid is recovered below 40DEG C. The wood frog spawn polysaccharides have mouse lymphocyte conversion promotion effects on in vitro mouse spleens to different degrees, a good dose-effect relationship exists, and the polysaccharide extract with the concentration of 0.001[mu]g / mL still has a strong lymphocyte conversion promotion effect. In mouse in vivo experiments, a wood frog spawn polysaccharide sample has an obvious lymphocyte conversion promotion effect. In the field of the influences on cytokines in mouse serum, the influences of the wood frog spawn polysaccharides on the outputs of TNF-alpha and IL-1beta in the serum substantially change. The wood frog spawn polysaccharide extract can be used to prepare immune medicines, and can also be used as a food additive. The extraction method and the extract solvent are not limited to that mentioned in the experiments and the embodiments.

Description

technical field [0001] The invention relates to a wood frog egg extract, which belongs to the field of traditional Chinese medicine technology and medical application, and relates to a preparation method of the wood frog egg extract and its effect on immune function. Background technique [0002] Chinese Rana [1] (R. t. chensinensis), according to taxonomy, belongs to Amphibians, Anura, Ranaidae, and Rana. It is commonly known as frog and Hash toad in the local area. We observe the Chinese forest frog from the morphology. It has a flat head, dark brown and black body color, and rough body surface. The general body length is 60-80mm, and individual individuals There is a difference. Another feature is that "the back folds are folded on the forehead". It can be observed that there are many black spots on the back folds, and this feature is also found on the back. In addition, the observation of the tympanic membrane reveals that there are large triangular black spots, and bl...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61K31/715A61P37/04
Inventor 卫功庆陈大勇蔡凤坤杨靖李硕李慧萍张连学
Owner 卫功庆
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