Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Natural antibacterial agent-recombinant chicken-beta defensins Gal-9-Gal-8 double-molecule protein preparation method

A technology of gal-9-gal-8, a natural antibacterial agent, which can be used in medical preparations containing active ingredients, food preparation, peptide/protein ingredients, etc., and can solve problems such as antibiotic residues, human health and environmental threats

Inactive Publication Date: 2007-08-01
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the widespread use of antibiotics in feed, antibiotics remain in livestock and livestock products, and cause antibiotic resistance in animals, posing a great threat to human health and the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Natural antibacterial agent-recombinant chicken-beta defensins Gal-9-Gal-8 double-molecule protein preparation method
  • Natural antibacterial agent-recombinant chicken-beta defensins Gal-9-Gal-8 double-molecule protein preparation method
  • Natural antibacterial agent-recombinant chicken-beta defensins Gal-9-Gal-8 double-molecule protein preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The utility model is described in more detail below in conjunction with accompanying drawing example:

[0029] 1. Design specific primers according to the published chicken-β defensin Gal-8 and Gal-9 gene sequences, Gal-9: upstream primer: 5'-GGA TCC CCG GAA TTC ATG CAG ATC CTGCCT CTC-3', downstream Primer: 5'-TCA GGA ATA CCA TCG GCT CCG GCA GCAGAA-3. Gal-8: Upstream primer: 5′-gga tcc atg aag atc ctc tgc ctg ctc ttc-3′, downstream primer: 5′-gaa ttc ctg cgc cgg aat ctt ggc aca gc-3′.

[0030] 2. Using RT-PCR method to amplify the chicken β-defensin Gal-9 gene (about 200bp) from the chicken tongue tissue, and clone it into the PMD-T vector, which was determined to be the chicken-β-defensin Gal-9 gene by sequence determination . (The shaded part is the target gene sequence, the rest is the vector, the primers are in the box, and the start codon and stop codon are in italics respectively) as follows:

[0031]

[0032] AAGCTTGGGAATCTCTAAGGATCCCCGGGTACCGAGCTCGAATTCG...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing natural antibacterial agent- recombination chicken- beta defensins Gal-9-Gal-8 bi-molecule protein. The invention employs molecular biology technology and colones chicken- beta defensins Gal- 8 and Gal-9 gene inside chicken and expresses defensins Gal-9-Gal-8 bi-molecule protein outside chicken body by employing proper expression host. The recombined chicken- beta defensins Gal-9-Gal-8 bi-molecule protein is characterized in that: (1) it can inhibit and kill bacillus coli, salmonella and salmonella and so on, (2) it can resist avian infectious brunchitis and newcastle disease virus, (3) it can increase the antibody for avian infectious brunchitis in dimefox by 10-20%, antibody for newcastle disease virus by 10-20% and increase chicken splenocyte lymphocyte conversion rate by 60-70%.

Description

1. Technical field [0001] The invention relates to a preparation method of a biological material, in particular to a preparation method of a biological material with broad-spectrum antibacterial activity and immune promotion effect. 2. Background technology [0002] Due to the widespread use of antibiotics in feed, the antibiotics remain in the carcasses and livestock products, and cause antibiotic resistance in animals, posing a great threat to human health and the environment. Therefore there is an urgent need for a natural pollution-free feed additive to be used in feed to promote animal growth and reduce feeding costs. Recombinant chicken-β-defensin is a small molecular polypeptide with broad-spectrum antibacterial activity that naturally exists in chickens. It has broad-spectrum antibacterial activity and immune promoting effect in vivo and in vitro, which provides a theoretical basis for the research and application of this invention. 3. Contents of the invention ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K19/00C07K14/465A61K38/16A61P31/00A23L1/305A23L33/17
Inventor 马得莹
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com