The invention relates to a method for studying the
structural diversity of a daqu bacterial
community, i.e. the denaturing gradient
gel electrophoresis (DGGE) technology, which belongs to the technical field of
microbial ecology and mainly comprises the following steps that: 1), daqu genomic deoxyribonucleic acid (
DNA) is directly extracted; 2), bacterial universal primers are selected, and specific
DNA fragments in the bacterial
ribosome DNA are amplified; 3),
polymerase chain reaction (PCR) products are separated through DGGE; 4), corresponding strips of microbes in the DGGE
fingerprint map are recovered in a gel
cutting way; 5), PCR is carried out again, products are connected to a T carrier, the blue and white spot screening is carried out, and positive clone
verification is carried out; and 6), the species information of the corresponding microbes of the DGGE strips is obtained through the sequence test. The method does not rely on the
molecular biology technology of the traditional culture method, has the characteristics of sensitivity, convenience and accuracy and solves the difficult problems to study some microbes incapable of being cultured or difficult to culture, and the method provides the technical basis for the
verification of the daqu bacterial
community structure and the discovery of new
wine brewing microbes or functional microbes.