Extracting method of polysaccharide and polyphenol plant genomes

Abstract

The invention belongs to the technical field of molecular biology and discloses an extracting method of polysaccharide and polyphenol plant genomes. The method includes: grinding plant materials, then adding a nucleic acid separation buffer solution and 2-mercaptoethanol, well mixing, and placing into water bath; centrifuging in a centrifuge, and then discarding supernate; adding a 1x PBS solution, well mixing on a grinding instrument in an oscillation manner, and discarding supernate after centrifuging; adding preheated 3x CTAB lysate into sediment, well mixing, and performing splitting decomposition under water bath; adding equal-volume phenol / chloroform / isoamyl alcohol mixed liquor, well mixing and centrifuging; taking supernate, adding equal-volume chloroform / isoamyl alcohol mixed liquor, well mixing and centrifuging; taking supernate, adding NaCl and icy isopropanol, well mixing, and precipitating for 1-3 hours; taking out and centrifuging, then washing sediment with ethanol, and adding TE for dissolving after air drying so as to complete the extracting process. The extracting method has the advantages that polysaccharides and polyphenols can be removed effectively, the influence of the polysaccharides and polyphenols on nucleic acid extraction is reduced, and DNA quality and concentration are increased.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and particularly relates to a method for extracting plant genomes. Background technique [0002] Genomic DNA extraction technology is a basic technology in genetics and molecular biology research. DNA extraction is an important step in molecular analysis and plays an important role in obtaining high-resolution results from gel-based systems. High-quality DNA is the basis for obtaining accurate test results. [0003] There are many DNA extraction methods, such as CTAB method, high salt and low pH method, SDS method, phenol method and the use of extraction kits. However, for most plants with thick leaves and juicy leaves, a large amount of polysaccharides and other secondary metabolites, it is difficult to extract high-quality DNA by conventional methods, so it is necessary to improve conventional isolation methods. [0004] CTAB is a detergent that can break cell walls and cell membran...

AI Technical Summary

Problems solved by technology

However, the concentration of genomes extracted by these methods is very low, and most of them are seriously polluted, which cannot meet the requirements of third-generation sequencing

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