31 results about "Z chromosome" patented technology

A DNA editing agent is disclosed which comprises a first nucleic acid sequence for eliciting in an inducible manner a lethal phenotype of male chick embryos in an egg of a bird and a second nucleic acid sequence for directing said nucleic acid sequence for effecting said lethal phenotype to a Z chromosome of a cell of the bird. Use of the DNA editing agent is also disclosed.

The invention discloses a target sequence suitable for transgene fixed point insertion of silkworm W-chromosome and a locus and application thereof. The nucleotide sequence of the target sequence is shown in SEQ ID NO.19 and is a specific sequence in silkworm W-chromosome, and Z-chromosome and autosome do not comprise the sequence; the target sequence inserted by a foreign gene can be expressed normally; therefore, the target sequence can be used as the fixed point insertion locus of the foreign gene in the silkworm W-chromosome so as to prepare W-chromosome chain transgenic silkworms, and has significance to identification of male and female silkworms.

The invention provides a cynoglossus semilaevis sex chromosome-linked DNA segment and application thereof. A sequence of a DNA segment on a Z chromosome is SEQ ID NO:1; a sequence of a DNA segment on a W chromosome is SEQ ID NO:2. The DNA segment is used for designing a molecular marker for identifying the genetic sex of cynoglossus semilaevis. Two Z-W chromosome homologous and different DNA segments are screened from cynoglossus semilaevis whole genome information, a primer is designed for identifying the genetic sex of the cynoglossus semilaevis, and the genetic sex of the cynoglossus semilaevis can be distinguished quickly, accurately and effectively. By adopting a method, specific purpose stripes can be amplified in male and female individuals and can be distinguished by agarose electrophoresis, the time for accurately identifying the genetic sex of the cynoglossus semilaevis is shortened, the method is suitable for identifying the genetic sex of the cynoglossus semilaevis in a simple environment of a farm, and detection time and cost are reduced.

The invention first provides a Cynoglossus semilaevis gender specific microsatellite marker, and comprises a microsatellite marker located in the Z chromosome, and the nucleotide sequence is SEQ ID NO:1. The invention also provides primers designed based on the above microsatellite marker, and the sequences of the upstream primer and the downstream primer are SEQ ID NO:2 and SEQ ID NO:3 respectively. The invention screens a Cynoglossus semilaevis gender specific microsatellite marker, and the Cynoglossus semilaevis gender specific microsatellite marker is subjected to SCAR conversion. Primers marked by SCAR are designed for Cynoglossus semilaevis heredity gender identification. Female, male and superfemale individuals of Cynoglossus semilaevis can be distinguished rapidly, accurately and effectively. Because the marker has characteristics that objective straps can be amplified in females and males and the objective straps can be distinguished with agarose electrophoresis, the time for accurate identification of Cynoglossus semilaevis heredity genders is shortened obviously, the on-site batch identification of Cynoglossus semilaevis heredity genders in a culturing farm of Cynoglossus semilaevis can be carried out, the detection cost is saved, and the work efficiency and accuracy of on-site detection of Cynoglossus semilaevis heredity genders in a culturing farm are raised.

The invention discloses a novel application for PRLR and a method thereof. In the novel application, copy number variation between a candidate gene of a feathering growth gene PRLR and a reference gene PCCA is authenticated by adopting a fluorescent quantitation PCR method according to a linkage relationship between a 176,324bp tandem repeat sequence existing in a Z chromosome and chicken fast andslow feather sites; the gene type can be judged simply and visually according to the difference between Ct values after finishing of the reaction, so that chicken fast and slow feather gene type, slow feather homozygosity / heterozygosity and male and female of chickens can be simultaneously distinguished; meanwhile the experiment is easy and convenient to operate, so that the breeding time and cost of the fast and slow feather pure chicken can be greatly shortened and lowered.

The present invention provides a screening method, a kit and applications of a cynoglossus semilaevis gunther sex conversion genetic control site. The screening method comprises: carrying out full-genome molecular marking on genetic female cynoglossus semilaevis gunther to obtain a plurality of SNP loci; and carrying out full-genome association analysis on the plurality of the SNP loci by adopting whether the transgenic female fish has sex conversion as the trait to obtain the cynoglossus semilaevis gunther sex conversion genetic control site. According to the present invention, from the perspective of the genetic control for controlling the sex conversion of the female fish, the full-genome analysis is performed by using the correlation between the unique SNP loci of the transgenic female fish and the sex conversion so as to find the molecular switch for controlling the sex conversion of the genetic female fish, wherein the molecular switch is the 6676874 site nucleotide on the Z chromosome; and by using the site to control the copulation method of the male cynoglossus semilaevis gunther and the female cynoglossus semilaevis gunther, the proportion of the female cynoglossus semilaevis gunther being not subjected to sex conversion in the artificial breeding cynoglossus semilaevis gunther progeny is increased so as to improve the breeding production benefits.

The invention discloses a photoelectric detection method for poultry gender. The photoelectric detection method comprises the following steps: horizontally fixing a to-be-detected egg collected by mating of a female duck with W chromosome containing GFP or a female duck with Z chromosome containing GFP and a male duck without GFP, carrying out standing for a few seconds until the interior of a blastoderm is completely static, and allowing the blastoderm to be able to freely rotate at the top part; and selecting 20 to 50 sites on an eggshell in a longitudinal axis plane perpendicular to the egg, and carrying out fluorescence measurement, wherein a correlation between a fluorescence measurement position and fluorescence intensity comprises two unique forms. The photoelectric detection methodprovided by the invention has the following beneficial effects: identification of a female embryo egg can be carried out under the condition of no damage to a breeding egg, is fast and effective andhas an accuracy rate reaching 100%.