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Novel application for PRLR gene and method thereof

A new application, genetic technology, applied in the field of molecular identification of fast and slow feathers of chickens, can solve the problems that hatching chickens cannot directly distinguish between male and female, and the test operation is cumbersome, so as to reduce the number of roosters, shorten the cultivation time, and save cultivation cost effect

Active Publication Date: 2018-04-20
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these two methods can distinguish fast feather and slow feather genotypes, they still cannot directly realize the distinction between male and female chicks; these two methods require enzyme digestion electrophoresis or electrophoresis to determine the genotype after PCR amplification. The operation is relatively cumbersome

Method used

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  • Novel application for PRLR gene and method thereof
  • Novel application for PRLR gene and method thereof
  • Novel application for PRLR gene and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Obtain chicken blood for DNA extraction

[0028] Chicken blood (5 μL) or tissue (30 mg) was taken, and genomic DNA was extracted using the Blood / Tissue / Cell Genome Extraction Kit (DP304) of Tiangen Biochemical Technology Co., Ltd.

[0029] 2. Design primers according to chicken PRLR and PCCA genes for fluorescent quantitative PCR amplification

[0030] Select the candidate gene PRLR gene of feather speed gene, design primer PCR amplification positive object: 5'-CCGTGGACAACATTCAATATCACT-3', reverse primer is 5'-GGATCCGAGCTGTTACTTCCAA-3'; internal reference gene PCCA gene PCR amplification forward primer: 5 '-CAGACACACAGAGCCCATCTCT-3', reverse primer: 5'-TGGAGCAGTGGTGGCTGTT-3', the primer was synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd. and diluted to a working concentration of 10pmol / L.

[0031] The PCR reaction system is 10 μL: 5 μL 2x SuperReal PreMix (SYBR Green, TAKARA), 3.2 μL ddH 2 O, 0.4 μL of upstream and downstream primers, 1 μL of DNA template. ...

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Abstract

The invention discloses a novel application for PRLR and a method thereof. In the novel application, copy number variation between a candidate gene of a feathering growth gene PRLR and a reference gene PCCA is authenticated by adopting a fluorescent quantitation PCR method according to a linkage relationship between a 176,324bp tandem repeat sequence existing in a Z chromosome and chicken fast andslow feather sites; the gene type can be judged simply and visually according to the difference between Ct values after finishing of the reaction, so that chicken fast and slow feather gene type, slow feather homozygosity / heterozygosity and male and female of chickens can be simultaneously distinguished; meanwhile the experiment is easy and convenient to operate, so that the breeding time and cost of the fast and slow feather pure chicken can be greatly shortened and lowered.

Description

technical field [0001] The invention belongs to the technical field of animal breeding, and in particular relates to a molecular identification method and application of fast and slow feathers of chickens. Background technique [0002] Male and female identification is an important technology in modern chicken production. It is widely used in the production of commercial layer chickens, broiler chickens and breeders to eliminate male chicks to save feeding costs, separate sex management and provide single-sex breeder chickens to protect breeding results. In the production of local chicken breeds, there is a big difference in the growth and development speed of males and females, and group feeding management is more conducive to improving economic benefits. [0003] Anal turning identification and self-sexing are two commonly used methods for male and female identification. Anal turning identification is based on the difference between male and female degenerated copulatory ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11C40B40/08
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2545/101C12Q2531/113C12Q2563/107
Inventor 张龚炜左福元张文秀吴雨徽刘安芳
Owner SOUTHWEST UNIVERSITY
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