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Cynoglossus semilaevis sex reversal genetic control locus, kit containing same and application of locus

A technology of semi-smooth tongue sole and position control, which is applied in the directions of application, climate change adaptation, microbial determination/inspection, etc., can solve the problems of reducing the reversal rate of seed sex, increasing the breeding cost of semi-smooth tongue sole, etc., and achieves the effect of improving the efficiency of aquaculture production.

Active Publication Date: 2019-06-04
CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

The study found that the proportion of genetic females with sex reversal in the cultured semi-smooth tongue sole population is relatively high, and the growth speed and individual size of pseudo-male fish with sex reversal are similar to those of genetic males, which increases the breeding cost of semi-smooth tongue sole. Therefore, how to improve The female rate of seedlings and how to reduce the sexual reversal rate of seedlings have become the focus of current research

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  • Cynoglossus semilaevis sex reversal genetic control locus, kit containing same and application of locus
  • Cynoglossus semilaevis sex reversal genetic control locus, kit containing same and application of locus
  • Cynoglossus semilaevis sex reversal genetic control locus, kit containing same and application of locus

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[0030] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below in conjunction with examples.

[0031] Previous studies by inventors Jiang and Li found that the mutation of Cyn_Z_6676874 (A>T) was significantly associated with sex reversal in half-smooth tongue sole. When the genotype is Z A When W, no reversal occurs, when the genotype is Z T At W, the individual is reversed, and this locus explains 82.7% of the variance of the entire phenotype. But there are still 17.3% of the remaining variation. Therefore, further research on the genetic control of sex reversal is still needed to find new control loci and apply them to the artificial breeding of tongue sole to improve the economic benefits of tongue sole.

[0032] The inventor selected 182 Zs based on the Cyn_Z_6676874 site T The W-type genetic female fish w...

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Abstract

The invention provides a cynoglossus semilaevis sex reversal genetic control locus, a kit containing the same and an application of the locus. The cynoglossus semilaevis sex reversal genetic control locus includes one or more following nucleotide loci on a Z chromosome: nucleotide loci at 8564889th, 8565011st, 8565016th, 8565018th, 8565031st, 8565187th, 8565217th and 8565235th and nucleotide locibetween 8565220th and 8565221st on the Z chromosome. From the perspective of genetic control of female fish sex reversal, a new molecular switch for controlling sex reversal of genetic female fishes is found out. Any one or more newly discovered genetic loci can control the mating mode of male and female cynoglossus semilaevis, and the proportion of female fishes that do not undergo sexual reversal in the offspring of artificially bred cynoglossus semilaevis is increased, and thus the production efficiency of breeding is improved.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a sex reversal genetic control site in tongue sole, a kit containing it and an application thereof. Background technique [0002] Semi-smooth tongue sole (Cynoglossus semilaevis) belongs to Osteichthyes, Pleuronectiformes, Cynoglossidae, Cynoglossus. The sex chromosomes of half-smooth tongue sole are ZW / ZZ female heterogamy, the growth speed of female and male fish is quite different, the growth speed of female fish is 2-4 times that of male fish, and the male fish cannot reach the commercial specification after two years of cultivation , and there is a one-way reversal phenomenon of genetic female fish to phenotypic male fish at the seedling stage. The study found that the proportion of genetic females with sex reversal in the cultured semi-smooth tongue sole population is relatively high, and the growth speed and individual size of pseudo-male fish with sex reversal are similar to t...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11A01K61/10
CPCY02A40/81
Inventor 李恒德蒋丽崔瑜王伟峰王悦玲
Owner CHINESE ACAD OF FISHERY SCI
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