Method for identifying sex of schistosoma japonicum cercariae with multiplex PCR method

Abstract

The invention discloses a method for identifying sex of schistosoma japonicum cercariae with a multiplex PCR method and belongs to the technical field of biological detection. A pair of primers is designed for bisexual Z chromosome of schistosoma japonicum, a pair of primers is designed from a characteristic amplification area of a female sequence in W chromosome, and a multiplex PCR system is constructed and can identify male and female cercariae simultaneously. The whole process of identifying the sex of cercariae takes about 4 h with the multiplex PCR method, and the cercaria sex identification time is substantially shortened as compared with existing reports. On one hand, time for establishing a schistosoma japonicum infected animal model is saved, and on the other hand, a required experiment animal model can be established more accurately according to the sex of the cercariae.

Description

technical field [0001] The invention relates to a method for identifying the sex of schistosoma japonicum cercariae by using multiple PCR method, belonging to the technical field of biological detection. Background technique [0002] Schistosoma japonicum is the only trematode with bisexual chromosomes, with 8 pairs of chromosomes, of which the female chromosome is ZW and the male chromosome is ZZ. Although there is obvious dimorphism in the adult stage, females and males can be distinguished by naked eyes, but it is difficult to distinguish males and females in the infection stage, that is, the cercariae stage. Accurate and efficient identification of the sex of schistosomiasis cercariae will help to make a correct evaluation of the genetics, epidemiology and pathological research results of schistosomiasis, and it will be helpful for the research and development of anti-schistosomiasis drugs, accurate evaluation of actual infection and control of schistosomiasis. period i...

AI Technical Summary

Problems solved by technology

However, this method only designs primers for the female-specific amplification region, and cannot accurately identify the sex of cercariae.

For example, for samples without amplified bands, it is impossible to determine that the cercariae escaped from the oncomelania are male cercariae, and the possibility of extracting DNA from the sample must also be ruled out.

For samples with specific amplification bands, it is also impossible to confirm that the sample is a single female cercariae, and the mixed infection of female cercariae and male cercariae must also be ruled out

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