Gender-specific microsatellite marker for Cynoglossus semilaevis and application of same in identification of superfemale Cynoglossus semilaevis

Abstract

The invention relates to a gender-specific microsatellite marker for Cynoglossus semilaevis and the application of same in the identification of superfemale Cynoglossus semilaevis. The Gender-specific microsatellite marker for the Cynoglossus semilaevis is characterized in that the nucleotide sequences of a microsatellite marker are respectively SEQ ID NO: 1 and SEQ ID NO: 2; and the upstream and downstream primer sequences of a primer used for amplifying the specific microsatellite marker are respectively SEQ ID NO: 3 and SEQ ID NO: 4. The primer is used for identifying individual female or male Cynoglossus semilaevis, the amplified product segment of the superfemale Cynoglossus semilaevis is 218 bp, and the amplified product of the female Cynoglossus semilaevis is two DNA segments, the sizes of which are respectively 216-220bp and 204-208 bp, and the amplified product of the male Cynoglossus semilaevis is one DNA segment, the size of which is 204-208 bp. The invention screens the specific microsatellite markers for W and Z chromosomes of the Cynoglossus semilaevis and designs the microsatellite primer. ZW female Cynoglossus semilaevis, WW superfemale Cynoglossus semilaevis and ZZ male Cynoglossus semilaevis can be fast identified by utilizing the microsatellite marker, thereby solving the problem of hard identification of the WW superfemale Cynoglossus semilaevis and the normal ZW male Cynoglossus semilaevis during the sex control and the unisexual seed cultivation of the Cynoglossus semilaevis.

Description

technical field [0001] The invention belongs to the technical field of fish sex identification and sex control in aquatic biology technology, and in particular relates to a sex-specific microsatellite marker of half-smooth tongue sole and its application in identification of super-female fish. Background technique [0002] Cynoglossus semilaevis (Cynoglossus semilaevis) is a large bottom fish with warm water in offshore waters. Its meat is tender and nutritious, and it is deeply loved by consumers. It is one of the leading marine fish species in my country. However, the growth rate of female and male individuals of semi-smooth tongue sole is very different, and the growth rate of females is 2-4 times faster than that of males. Due to the slow growth and small size of male individuals, the aquaculture output of tongue sole is reduced and the cost of breeding is increased, which seriously affects the promotion of tongue sole seedlings and the formation of aquaculture industry....

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a sex microsatellite marker of half-smooth tongue sole and its application in the identification of superfemale fish, that is, to obtain the molecular markers for the identification of half-smooth tongue sole WW superfemale and ZW female individual by screening sex-specific microsatellite markers. This marker can solve the problem of not being able to quickly identify ZW females and WW super females in sex control and all-female breeding studies of half-smooth tongue sole, so as to make up for the shortcomings of existing technologies

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