Omega-aminotransferase mutant gene and application thereof
A transaminase and mutant technology, applied in the field of molecular biology, can solve the problems of substrate and product inhibition, enzyme substrate specificity, enantioselectivity and/or low conversion rate, etc., to achieve the effect of improving efficiency
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Embodiment 1
[0026] In 2 mL of 20 mM pH 8.0 phosphate buffer, add 0.25 mg ω-TA, 5 μmol trifluoroacetone, 15 μmol L-aspartic acid and 0.1 μmol PLP, react at 25 °C for 1 h, and the conversion rate is 97.0 % of trifluoropropylamine.
Embodiment 2
[0028] In 2 mL of 20 mM pH 7.5 phosphate buffer, add 0.5 mg ω-TA, 10 μmol trifluoroacetone, 30 μmol L-alanine and 0.2 μmol PLP, react at 25 °C for 2 hours, and the conversion rate is 93.0% of trifluoropropylamine.
Embodiment 3
[0030] In 10 mL of 20 mM pH 8.0 phosphate buffer, add 2 mg ω-TA, 50 μmol trifluoroacetone, 150 μmol L-aspartic acid and 1 μmol PLP, react at 25 °C for 3 h, and the conversion rate is 92.0 % of trifluoropropylamine.
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