36 results about "Phagocytic index" patented technology

The invention discloses a health food for improving immunity and a preparation method thereof. The health food is prepared from the following raw materials in part by weight: 1 to 3 parts of dendrobium officinale, 2 to 6 parts of American ginseng and 6 to 18 parts of Chinese wolfberry. Pharmacological experiments prove that: the health food has an obvious effect of promoting an immune system, canobviously improve a phagocytic index of macrophage of immunosuppressed mice and increase coefficients of immune organs in nonspecifc immune experiments, can improve the conversion rate of lymphocytes, improve the level of immunoglobulin IgG, IgA and IgM in serum of the immunosuppressed mice and assist in conditioning immunodeficiency disease, and diseases such as weakened immune system, low resistance and the like.

The invention discloses a traditional Chinese medicine formula and a preparation method of the traditional Chinese medicine. According to the formula, the traditional Chinese medicine is prepared from sealwort, ginseng (artificial planted) and poria cocos in a weight ratio of (50-100): (5-40): (10-50). The traditional Chinese medicine is prepared by the following steps of drying; crushing; extracting; drying and the like and has the functions of enhancing immunity and relieving fatigue (boosting qi and nourishing yin). The traditional Chinese medicine has the effects of raising the half value (HC50) of hemolysin of mice serum to different extents and increasing the phagocytic index (a) of mice, which means that the traditional Chinese medicine can be used for enhancing the humoral immune function and mononuclear-macrophage function and exerting a function of boosting immunity. The traditional Chinese medicine can be prepared into various dosages such as tabletted candies, tea bags, instant tea, troches, capsules, powder, pills, granules, oral liquids, injections and the like by using a modern preparation technology, and has good development and application prospects in preparation of drugs, health care foods or foods.

The invention relates to protein peptide powder for enhancing immunity and a preparation method and application thereof. The protein peptide powder is prepared from the following raw materials by weight: 30%-45% of marine fish skin collagen oligopeptide powder, 30%-50% of soybean oligopeptide powder, 10%-20% of Isomaltooligosaccharide, 8%-13% of polydextrose and 0%-0.5% of a sweetener. The protein peptide powder can increase macrophage phagocytic rate and phagocytic index and serum hemolysin content, enhances lymphocyte proliferation and NK cell activity, promotes antibody formation cell number increase, improves the body's immunity, and enhances body immunity.

The invention discloses a composite feed for improving immunity of herring. The feed comprises the following components by weight portion: 3-6 parts of a fish meal, 15-25 parts of a soybean meal, 2-5 parts of a meat and bone meal, 4-6 parts of wheat bran, 4-8 parts of rice chaff and 5-10 parts of compound vitamin; and the feed also comprises 5-15 parts of Chitosan Oligosaccharide and 10-20 parts of konjac mannan oligosaccharide. The feed provided by the invention has effects of enhancing body function and immunity of herring, and the effects are reflected in the significantly increased immune indexes including superoxide dismutase, phagocytic percentage and phagocytic index of herring; and the feed provided by the invention has extensive component sources and low price, and is suitable for large-scale cultivation.

The invention discloses a method for identifying and researching the function and capability of cells for phagocytizing exogenous foreign bodies. The method comprises the following steps: preparing an infection source; performing mixed culture; directly observing the whole process that the researched cells phagocytize the infection source and cysts under a fluorescence microscope; and analyzing changes of phagocytic capability of the researched cells under different conditions so as to obtain phagocytic indexes. The method is characterized in that in the step of preparing the infection source, escherichia coli for expressing green fluorescent protein is taken as the infection source. In the method, the escherichia coli capable of expressing the green fluorescent protein is taken as the infection source so as to research the phagocytosis of various cells; and the method is intuitive, accurate, fast and convenient and can be also used for distinguishing the types of cells with a phagocytic function.

The present invention discloses a Dendrobium composition and preparation method with enhanced immune function.Dendrobium compositions are composed of Dendrobium, astragalus, jujube, wolfberry, longan meat, Poria, yellow essence, and hybrid maltose, which can exert the overall adjustment advantage of compound traditional Chinese medicine. It can regulate the body's immune function and small toxic side effects.Experiments have confirmed that it can promote the proliferation and transformation of mice lymphocytes and the devouring ability of macrophages, which can improve the activity of serum hemolytin levels, carbon outlines, and NK cells.In human health.

An application of lachnum water-soluble melanin SLIM404 in raising immunity. The invention belongs to the field of immunity in medicine and provides lachnum melanin SLIM404 and specifically relates to an application of the melanin in raising immunity and in preparing medicines for raising immunity. The melanin is intracellular melanin of lachnum YM404 and is a water-soluble lachnum melanin obtained after sulfur trioxide-pyridine modification. The melanin has an effect of raising immunologic function, and raises immunity by promoting thymus organ development, raising phagocytosis percentage and phagocytic index of macrophage, enhancing B cell-mediated humoral immunity, promoting a T cell-mediated immune response, increasing activity of an in-vivo antioxidant enzymatic system and removing excess free radicals and lipid peroxide in the immunocompromised body and the like. A medicine prepared from the melanin has various routes of administration, is safe and nontoxic, is easy to prepare and is suitable for large-scale popularization and application.

The invention provides a composition. The composition is prepared from the following components in parts by weight of 1 to 8 parts of herba cistanche, 1 to 8 parts of radix astragali seu hedysari, 1 to 8 parts of fructus ligustri lucidi and 1 to 8 parts of fructus lycii. The medicinal materials of the composition supplement each other and can be taken as a health-care product for a long time. Pharmacodynamic experiments prove that the composition can significantly enhance the delayed allergic reaction and carbon clearance phagocytic index of mice and enhance the phagocytic function of peritoneal macrophages, and has the effect of significantly enhancing immunity.

The invention discloses a preparation method of functional yam-flavored wine and belongs to the technical field of preparation of functional wine. The preparation method comprises steps as follows: preparation of a yam saccharification liquid, preparation of glutinous rice saccharification mash, dry yeast activation, mixed fermentation, clarification, ageing and sterilization. The yam-flavored healthcare wine has stable quality, mellow taste and good healthcare effects. Yam has the functions of reducing blood glucose, resisting oxidation, delaying senescence and resisting tumor and mutation and can improve the intestinal function. Yam polysaccharide can enhance the capability of lymphocyte proliferation, has a function of promoting antibody generation and can obviously increase the macrophage phagocytosis percentage and phagocytic index under the condition of low immune function caused by cyclophosphamide, promote formation of mature hemolysin and hemolysis plaque and conversion of lymphocyte and remarkably increase the peripheral T-lymphocyte ratio.

The invention provides a probiotic composition suitable for intestines and stomach, and relates to the technical field of probiotic compositions. The probiotic composition suitable for intestines and stomach comprises a probiotic group, an unsaturated fatty acid group, a plant polysaccharide group, protease, lipase and a vitamin group. The unsaturated fatty acid group composed of linoleic acid, linolenic acid and arachidonic acid cooperates with the probiotic group to achieve the effects of promoting blood circulation and reducing the content of cholesterol in blood; the plant polysaccharide group composed of rhamnose, fucose, mannose and uronic acid cooperates with the probiotic group to improve the phagocytic index of macrophages of a body, and moreover, production of antibodies can be stimulated, so that the immune function of the human body can be enhanced; and the vitamin group consisting of a vitamin K1 and a vitamin U, and the protease and the lipase cooperate with the probiotic group, so that the composition can resist gastric ulcer, protect and repair gastric mucosa tissues, keep gastric cells active and vigorous, and reduce the probability of lesion.

The invention relates to a method for preparing hyriopsis cumingii polysaccharide, which comprises following steps: crushing hyriopsis cumingii, extracting with hot water, filtering with 60-100 orders, condensing filter liquor, then, adding ethanol to deposit, re-dissolving deposition with hot water, adjusting the pH value to be 7-10, adding remixed proteinase 0.3%-0.6% according to a protein weight gage in re-dissolved materials, carrying out enzymatic hydrolysis for 4-8 hours under the temperature of 30-60DEG C, wherein components of the remixed remixed proteinase comprise: alkaline protease (Alcalase) and compound protease (Protamex) which are mixed equivalently, killing enzyme for the materials after the enzymatic hydrolysis under the temperature of 80-90DEG C, using the ethanol to deposit for the second time, drying the deposition in the vacuum, crushing over 60 orders, and obtaining products of the hyriopsis cumingii polysaccharide. Experiments show that the hyriopsis cumingii polysaccharide which is prepared by the method of the invention has the ability to induce the mouse spleen lymphocytes to proliferate, the hyriopsis cumingii polysaccharide can increase the volume value of mice serum hemolysin and can increase the phagocytic rate and the phagocytic index of chicken-red cells phagocytosis in mouse peritoneal, and the hyriopsis cumingii polysaccharide has a pluralityof biological activity and has the application prospect as medicinal resources and healthy food.

The invention discloses medicine composition for improving the immune function of a human body and a preparation method of the medicine composition. The medicine composition is prepared from components of raw materials in parts by weight as follows: 8-80 parts of ficus hirta, 5-60 parts of radix angelicae sinensis and 1-30 parts of ganoderma lucidum. Experiments indicate that the medicine composition has the function of improving the immune function of the human body, the influence of the medicine composition on carbon particle clearance indexes, phagocytic indexes, thymus indexes, spleen indexes and other indexes of immunosuppressed mice is higher than that in two-two compatibility group, and results indicate that the components have a synergistic function. Besides, the medicine composition can remarkably reduce the TC and TG level in serum of the mice and has a blood fat reduction function.

The invention discloses a preparation method and application of liver cancer immunogen Z1, belonging to the technical field of biological engineering. The preparation method provided by the invention comprises the following steps of: inoculating H22 liver cancer cells in a culture medium, simultaneously adding lentinan to co-culture, taking out after culturing, repetitively freezing, thawing and crushing the cells, centrifugally separating to obtain supernate, adding ammonium sulphate into the supernate to salt out, and separating and collecting precipitate after salting out so as to finally obtain the liver cancer immunogen Z1. If a mouse is inoculated with the immunogen prepared by the preparation method provided by the invention, the survival rate of the mouse can be effectively prolonged; compared with a control group, the survival rate of the mouse inoculated with the immunogen prepared by the invention can be increased by 100%; the thymus index and the spleen index are up to 2.68 and 4.56 respectively; the phagocytic rate and the phagocytic index of macrophage are up to 52% and 3 respectively; and the serum antibody titer of the mouse is up to 25600x.

The invention discloses a strain of animal bifidobacteria NSY0201 and its application in preparing health products or medicines for enhancing body immunity. The classification of the animal Bifidobacterium NSY0201 is named Animal Bifidobacterium Bifidobacterium animalis , preserved in China Center for Type Culture Collection, the preservation number is CTCC M 2021629, its nucleotide sequence is shown in SEQ ID No.1, and has the function of high-yield extracellular polysaccharide. The animal Bifidobacterium NSY0201 and its freeze-dried powder can enhance the body's immune function by inhibiting DNFB-induced DTH, increasing the serum hemolysin level, phagocytosis index and NK cell activity, and accelerating the proliferation of lymphocytes in mice. and disease resistance, and it is safe to use, can be used to prepare health products or medicines, and has good application prospects.

The invention relates to a feed additive. The feed additive is prepared from a volatile oil mixture and a carrier, wherein the mass ratio of the volatile oil mixture to the carrier is 1 to (4-8), the volatile oil mixture is prepared by mixing turmeric volatile oil, fiveleaf gynostemma herb volatile oil and atractylodes rhizome volatile oil, and the carrier is beta-cyclodextrin. The invention further relates to a preparation method and application of the feed additive in a feed for aquatic animals. Compared with the prior art, if the feed additive disclosed by the invention is applied to the feed for the aquatic animals, the antioxidant ability of the aquatic animals can be notably improved, the total antioxidant ability of serum and the activity of superoxide dismutase can be improved, the antioxidant ability of organisms can be improved, the oxidative damage of the organisms can be avoided, the immunity of the aquatic animals can be improved, the lysozyme vitality and complement vitality in the serum of the aquatic animals can be improved, the leucocyte phagocytic index in the serum can be increased, the health of the organisms can be improved, the growth rate and the survival rate of the aquatic animals can be increased, the growth is promoted, and a theoretical basis is laid for the development of the green raising of the aquatic animals.

The invention relates to the technical field of health products, in particular to a compound for improving immunity and a preparation method thereof. The compound is prepared by combining apple stem cell freeze-dried powder with paecilomyces hepiali fungus powder, and can be used for remarkably improving immunity of a human body. Tests show that after gavage in a mouse with the compound, the monocyte-macrophage phagocytic index of the mouse can be increased from 1.19 plus or minus 0.17 to 1.43 plus or minus 0.23, a significant difference is realized, and the NK cell activity of the mouse is improved from 26.84% plus or minus 13.52% to 28.96% plus or minus 13.65%. After the compound is taken by 300 low-immunity people, survey results show that 99% of the people consider the compound to be an efficacious medicine, and the percentage is remarkably higher than that obtained from a control group.

The invention discloses a method for detecting the activity of abdominal cavity phagocytic cells by a flow cytometry, which aims at providing a method for fast, accurately, simply and easily detecting the activity of the phagocytic cells through the quantitative analysis on the number of phagocytic yeasts of the phagocytic cells on the basis of eliminating the influence of unendocytosed fluorescence yeast cells on the analysis results. The method comprises the following steps: preparing beer yeast cell freeze-dried powder; using FITC to mark the beer yeast cells; carrying out incubation on the suspension liquid of the fluorescence marked beer yeast cells and the blood serum of the mice at the room temperature; adding the suspension liquid of the abdominal cavity phagocytic cells into a hole of a culture plate; adding the fluorescence marked beer yeast cells after the incubation with the blood serum of the mice and the identical suspension liquid of the abdominal cavity phagocytic cells into the rest holes in the cell culture plate; carrying out wall adherence incubation on the cell culture plate; and using the flow cytometry to obtain data and carrying out analysis on the data. The method of the invention accurately measures the phagocytic rate and the phagocytic index, and has good repetitiveness.

The invention relates to a drug combination with an antineoplastic function, which comprises artificial bear gall, artificial musk, astragalus hoangtchy, boswellia carteri, myrrh, rhizoma paridis and radix pseudoginseng and can obviously control the growth of transplantation tumor MGC-803 of S180 tumor-bearing mice and BALB / C mice. Composition of stomach is filled in a rat and cyclophosphamidum is injected into an abdominal cavity so as to prolong the survival time of S180 mice with ascites tumor. When used with the cyclophosphamide, the composition of stomach has a synergy trend for the survival time of S180 mice with ascites, a certain improvement for immune function reduction caused by the injection of cyclophosphamidum and a certain enhancement for phagocytic clearance capacity of carbon granules, and increases pyagocytic index (K) and contents of serum hemolysin. The drug combination can be used for the treatment of primary gastric cancer, block blood poisoning of traditional Chinese medicine and righteousness weak disorder, and can be prepared as drugs or health food with antineoplastic drugs, food and health products.

The invention discloses a preparation method and application of liver cancer immunogen Z1, belonging to the technical field of bioengineering. The method provided by the present invention is to inoculate H22 liver cancer cells in the culture medium and add lentinan for co-cultivation, take out after the culture, repeatedly freeze-thaw and break the cells, centrifuge to obtain the supernatant, add ammonium sulfate to the supernatant for salting After the salting out, the precipitate was separated and collected, and finally the liver cancer immunogen Z1 was obtained. Utilizing the immunogen prepared by the preparation method provided by the present invention to inoculate mice can effectively prolong the survival rate of mice. Compared with the control group, the survival rate of mice inoculated with the immunogen prepared by the present invention can be increased by 100%. The index and spleen index reached 2.68 and 4.56 respectively, the phagocytosis rate and phagocytosis index of macrophages reached 52% and 3 respectively, and the mouse serum antibody titer reached 25600×.