Preparation method of hyriopsis cumingii polysaccharide
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A technology of spinnaker mussel and polysaccharide is applied in the biological field to achieve the effects of stable process, high extraction rate and enhanced immunity
Active Publication Date: 2011-06-22
ZHEJIANG GONGSHANG UNIVERSITY
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It has been reported that the crude polysaccharide components obtained from Trichogonaceae have the effect of enhancing the immune function of mice, using crude polysaccharides to extract ZBP-I and its application in the preparation of tumor drugs (Patent No. CN1544478A), Pearl Mussel Homogeneous polysaccharides ZBP-I and ZBP-II and their application in the preparation of hepatoprotective health care products and hepatoprotective drugs (patent number CN1935847A), using the concentration of 5% NaOH, the technology of extracting polysaccharides from S. , 2006, 28(5): 746-748), but the process of preparing polysaccharides from S. sinensis by enzymatic extraction has not been reported yet.
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Embodiment 1 3
[0014] Example 1 Extraction and preparation technology of polysaccharide
[0015] The jellyfish meat is mechanically crushed, extracted with hot water and boiled according to the raw material: water ratio of 1:4-6, the temperature is controlled at 90-100°C, and the time is 10-20 minutes; the filter is filtered with 60-80 mesh fine cloth, concentrated to one-eighth of the volume of the filtrate, then add 90-95% ethanol for precipitation, measure the alcohol content to be 60°-70°, place it for 10 hours, centrifuge at 3500rpm, recover ethanol from the supernatant, add 80-100% ethanol to the precipitation Redissolve in hot water at ℃, adjust the pH to about 7-10, add 0.3-0.6% compound protease (based on protein content), the composition of the compound protease is: alkaline protease (Alcalase), compound protease (Protamex), The two enzymes are mixed in equal amounts and hydrolyzed in a water bath at 30-60° C. for 4-8 hours. After enzymolysis, inactivate the enzyme at 80-90°C for ...
Embodiment 2 3
[0016] Example 2 The functional test of the refined polysaccharide of Trigonophylla japonicus enhancing immunity
[0017] (1) Effects of refined polysaccharides from S. sinensis on ConA-induced proliferation of mouse spleen lymphocytes
[0018] 1 Experimental materials:
[0019] The experimental ICR mice were provided by Shanghai Slack Experimental Animal Co., Ltd., the experimental animal license number is SCXK (Shanghai) 2007-0005, clean grade, female, weighing 20±2g. The feed was provided by Zhejiang Experimental Animal Center, and the standard GB14924-2001 was implemented. Detect the environmental conditions, the temperature is 20-23°C, and the relative humidity is 50-70%. The animals were acclimatized in the animal room environment for 3 days before the experiment.
[0020] 2-dose design:
[0021] The experiment set up 3 dosage groups and distilled water control group. The three doses weighed 0.6, 1.2 and 3.6g of the refined polysaccharide samples of Triangle sailfis...
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Abstract
The invention relates to a method for preparing hyriopsis cumingii polysaccharide, which comprises following steps: crushing hyriopsis cumingii, extracting with hot water, filtering with 60-100 orders, condensing filter liquor, then, adding ethanol to deposit, re-dissolving deposition with hot water, adjusting the pH value to be 7-10, adding remixed proteinase 0.3%-0.6% according to a protein weight gage in re-dissolved materials, carrying out enzymatic hydrolysis for 4-8 hours under the temperature of 30-60DEG C, wherein components of the remixed remixed proteinase comprise: alkaline protease (Alcalase) and compound protease (Protamex) which are mixed equivalently, killing enzyme for the materials after the enzymatic hydrolysis under the temperature of 80-90DEG C, using the ethanol to deposit for the second time, drying the deposition in the vacuum, crushing over 60 orders, and obtaining products of the hyriopsis cumingii polysaccharide. Experiments show that the hyriopsis cumingii polysaccharide which is prepared by the method of the invention has the ability to induce the mouse spleen lymphocytes to proliferate, the hyriopsis cumingii polysaccharide can increase the volume value of mice serum hemolysin and can increase the phagocytic rate and the phagocytic index of chicken-red cells phagocytosis in mouse peritoneal, and the hyriopsis cumingii polysaccharide has a pluralityof biological activity and has the application prospect as medicinal resources and healthy food.
Description
technical field [0001] The invention belongs to the field of biotechnology, relates to medicine and health food and its application, and specifically relates to a preparation method and application of the polysaccharide from the triangle sail, especially an enzymatic extraction and preparation process of the polysaccharide from the triangle sail. Background technique [0002] Hyriopsis cumingii (hyriopsis cumingii), commonly known as river mussels, pearl mussels, and triangle mussels, belongs to freshwater bivalve molluscs. Jiangxi and other provinces. The large-scale artificial breeding of freshwater pearls in my country has a history of more than 30 years. After the pearls are obtained from the culture of mussels, the mussel meat is used as leftovers. Except for a small amount of fresh food, most of them are simply processed and dried and crushed as feed. The waste is serious, and the economic use value is extremely low. If it is not handled in time, it will also cause pol...
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