Lactobacillus plantarum HG20 strain and application thereof

A technology of Lactobacillus plantarum and bacterial agent, applied in the field of Lactobacillus plantarum HG20 strain, can solve the problems of infection and side effects of RA patients, and achieve the effects of alleviating inflammation, improving the level, and reducing the diameter of ankle joints

Active Publication Date: 2020-04-17
JILIN MINGZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can improve the symptoms of RA and inhibit bone destruction to a certain extent, long-term use has strong toxic and side effects, such as inhibiting the immune function of the body and inducing serious adverse reactions such as infection in RA patients.

Method used

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  • Lactobacillus plantarum HG20 strain and application thereof
  • Lactobacillus plantarum HG20 strain and application thereof
  • Lactobacillus plantarum HG20 strain and application thereof

Examples

Experimental program
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preparation example Construction

[0025] The preparation method of a kind of bacterial agent of the present invention comprises the following steps:

[0026] Plantarum HG20 strain was expanded to 2L, centrifuged at 6000r / min for 5min, resuspended in normal saline, centrifuged again at 6000r / min for 5min, resuspended in normal saline, washed, centrifuged again at 6000r / min for 5min, collected the sludge, and dissolved in In 1L of freeze-drying protection agent, freeze-dry to obtain Lactobacillus plantarum HG20 starter, the number of bacteria is 1.0×10 10 CFU / g.

[0027] Among them, the lyoprotectant is obtained by dissolving 40 g of glucose, 5 g of sodium ascorbate, 80 g of fructooligosaccharide, and 30 g of trehalose in tertiary water, and distilling the volume to 750 mL.

[0028] A plant lactobacillus (L. plantarum) HG20 strain of the present invention can be used in the preparation of products with the function of preventing or treating rheumatoid arthritis. Its products can be food, health products or med...

Embodiment 1

[0039] The preparation of embodiment 1 plant lactobacillus (L.plantarum) HG20 strain

[0040] 1. Isolation of Lactobacillus plantarum strains

[0041] 1. Sample material: surface of cucumber skin.

[0042] Take 5g of cucumber peel, add 45mL of normal saline (10-fold dilution) and mix well, take 0.5mL of diluent, and dilute to 10 times with normal saline again. -3 , take 100μL liquid (10 -1 、10 -2 、10 -3 ) in solid MRS 5.2 medium for cultivation (cultivation temperature 37°C, cultivation time 48-72h). Pick a single colony and inoculate it on a solid MRS medium plate to continue culturing (cultivation temperature 37°C, culture time 48h), and repeatedly streak culture and purify the solid MRS medium plate (cultivation temperature 37°C, culture time 48h), and obtain multiple strains Pure cultured strains.

[0043] 2. Inoculate the multiple strains of purely cultured strains obtained above into liquid MRS medium for culture (culture temperature 37°C, culture time 14-18h), then ...

Embodiment 2

[0068] Embodiment 2 Effect of plant lactobacillus (Lactobacillus plantarum) HG20 strain of the present invention on collagen type arthritis model rats

[0069] 1. Establishment and grouping of animal models

[0070] Calf cartilage type Ⅱ collagen plus complete Freund's adjuvant was used as a modeling agent after being fully emulsified. 20 mg of bovine type Ⅱ collagen was dissolved in 10 mL of acetic acid with a concentration of 0.1 mol / L. The adjuvant is fully emulsified. After disinfecting the left and right paws of the rats with alcohol, inject 100 μL / rat intradermally at the pads of the left and right paws. After 7 days, booster injection. And 30 male SD rats were randomly divided into 3 groups: control group, model group and Lactobacillus plantarum HG20 group, 10 in each group. Seven days after the booster injection, the control group and the model group were given intragastric administration of normal saline 2mL / d, and the Lactobacillus plantarum HG20 group was intraga...

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Abstract

The invention discloses a Lactobacillus plantarum HG20 strain and an application thereof, and belongs to the field of functional food microorganisms. The Lactobacillus plantarum HG20 strain is preserved in China Center for Type Culture Collection on November 21, 2019 with the preservation number of CCTCC NO:M2019964. A collagen type rheumatoid arthritis rat model is adopted, and is intragastrically administered with the Lactobacillus plantarum HG20 strain for 21 d, and the result shows that the Lactobacillus plantarum HG20 strain can reduce the diameter of the ankle joint of a rat, reduce theinflammatory response, reduce the levels of inflammatory factors IL-1 beta and TNF-alpha and improve the level of an anti-inflammatory factor IL-10; the strain reduces the levels of C reactive proteinand immunoglobulin IgG and IgM during the attack period of rheumatoid arthritis; and the anti-inflammatory effect can be effectively achieved, so the symptoms of redness, swelling and pain of jointsare improved.

Description

technical field [0001] The invention belongs to the technical field of functional food microorganisms, and in particular relates to a strain of Lactobacillus plantarum HG20 and its application. Background technique [0002] Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint pain, joint capsule swelling, and lesions. The occurrence and development of RA are related to the release of a large number of inflammatory cytokines and inflammatory mediators. The main pathological change is chronic synovitis, which gradually manifests as cartilage and bone destruction, eventually leading to joint deformity and even loss of joint function in patients. It is the cause of labor loss in my country. and one of the leading causes of disability. [0003] Clinically, there is currently no fundamental treatment for RA. The current drug treatment mainly focuses on relieving pain, controlling the development speed of arthritis and preventing damage to articular car...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P29/00A61P19/02A23L33/135C12R1/25
CPCA23V2002/00A61K35/747A23L33/135A61P19/02A61P29/00C12N1/20C12N1/205C12R2001/25A23V2200/30
Inventor 崔伟东梁铁
Owner JILIN MINGZHIYUAN BIOTECH
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