44results about How to "Strong detection specificity" patented technology

The invention relates to a high sensitive blood plasma homocysteine testing kit that includes sample tube with stabilizer, reference substance and quality control serum of different Hcy thickness, DL- homocystine-D8 internal standard solution, reducer and trichloroacetic acid albumen precipitation. The invention has strong specificity for sample testing, and high sensitivity. It could be accurately and rapidly used in Hcy research.

The invention discloses a fluorogenic quantitative PCR detection kit for bovine ACTB gene transcription level. The kit comprises 2XSYBR GREEN MIX, a primer mixed liquor, a standard ACTB genetic template and ultrapure water. The kit of the invention can be used to measure the ACTB gene transcription level accurately, and possesses high specificity (Figure 1). The results of an amplification curve show that ACTB fluorescence signal value accords with a standard "S" typed curve, and the results of a melting curve show that the fluorogenic quantitation possesses high detection specifity. [0]

A detection method for detecting HPLC-ESI-MS / MS of 19 species of quinolone drugs simultaneously belongs to the antibiotic drug analysis technology field. The detection method comprises three steps: sample treatment, liquid-phase separation and mass spectrometric detection, wherein the 19 species of quinolone antibiotics in a tissue fluid is subjected to positive ion MRM scan; norfloxacin-D5 is taken as the internal standard; C18 chromatographic columns are adopted for separation; water-methanol containing formic acid is taken as the mobile phase for gradient elution; and the eluent is subjected to mass spectrometric detection. The detection method is characterized in that the organic solvent extraction method is adopted for carrying out the sample pretreatment according to the practical situation of the sample and the different detection sensitivity requirements; norfloxacin-D5 is taken as the internal standard; the C18 chromatographic columns are adopted for separation; and the water-methanol containing formic acid is taken as the mobile phase for gradient elution. The pretreatment process of the method is rapid and convenient, the detection result is reliable and sensitive, and the method is suitable for the analysis and the detection of the quinolone drug residues in animal food.

The invention discloses a method for detecting benzoic acid content in Kushen compound salicylic acid powder. The method uses high performance liquid chromatography and comprises the steps of: S1, preparation of a reference solution and a test solution comprises (1) preparation of the reference solution by taking a proper amount of analytically pure benzoic acid, accurately weighing, adding absolute ethyl alcohol to produce a solution as the reference solution containing 1.2 mg per 1 ml; (2) preparation of the test solution comprises taking 0.5g of Kushen compound salicylic acid powder, accurately weighing, adding 50ml into a volumetric flask, adding 40ml of absolute ethyl alcohol, sealing with plug, conducting ultrasonic treatment for 3-7 min, cooling, diluting with absolute ethyl alcohol to the mark, shaking evenly, filtering, and taking the filtrate as the test solution; and S2, detection: taking precisely 10 mul of the test solution and 10 mul of the reference solution, injecting into the liquid chromatograph, and measuring by using 0.03-0.07mol / L potassium dihydrogen phosphate solution-methanol as the mobile phase. The detection method provided by the invention is simple and easy to operation; the extraction of benzoic acid in the Kushen compound salicylic acid powder is thorough; and the detection result is accurate and reliable.

The invention belongs to the technical field of chloride ion detection, and particularly relates to a method for detecting the content of chloride ions in body fluid through dendritic gold nanoparticles. The method comprises the following steps: preparing a dendritic gold nanoparticle solution, and preparing a paper-based gold nano material; mixing a sample to be detected with a nitric acid solution with the concentration of 8-16 mol / L, heating in a water bath environment with the temperature of 40-60 DEG C, adding the paper-based gold nano material, reacting for 30-40 minutes, and qualitatively detecting chloride ions when the paper-based gold nano material fades; and performing light intensity value analysis on the reacted paper-based gold nano-material through mage J software to obtain an optical signal of the paper-based gold nano-material so as to quantitatively detect the content of the chloride ions in the solution to be detected. The method for detecting the chlorine ion content in the body fluid by using the dendritic gold nanoparticles has the advantages of multi-color change, high detection sensitivity, simplicity in operation, high speed and the like.

The invention discloses a nano- gold test paper film. The nano-gold test paper film is formed by loading nano-gold particles on the surface of a PVDF film. The invention also discloses an applicationof the nano-gold test paper film in chloride ion detection based on the nano-gold test paper film. A method for detecting chloride ions comprises the following steps: S1, mixing a sample to be testedwith a nitric acid solution; S2, taking the nano-gold test paper film, adding the nano-gold test paper film to the mixed solution obtained in step S1, carrying out a reaction for 5-75 min, and qualitatively detecting chloride ions when the nano-gold test paper film fades; and S3, carrying out light intensity analysis on the reacted nano-gold test paper film by Image J software to obtain an opticalsignal in order to achieve quantitative detection of the concentration of chlorine ions in a solution to be tested. The nano-gold test paper film has the advantages of high detection sensitivity, high speed and simplicity operation when applied to the detection of chloride ions.

The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W / V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.