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A kind of divalent copper ion ratio fluorescent probe and its preparation method and application

A ratiometric fluorescent probe, divalent copper ion technology, used in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., to achieve the effect of strong detection specificity, excellent detection limit, and no need for equipment

Inactive Publication Date: 2016-08-24
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the detection limit of the ratiometric fluorescent probes of the prior art to the detection of divalent copper ions can only reach 4.1×10 7 M, still can not meet the high-precision detection

Method used

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  • A kind of divalent copper ion ratio fluorescent probe and its preparation method and application
  • A kind of divalent copper ion ratio fluorescent probe and its preparation method and application
  • A kind of divalent copper ion ratio fluorescent probe and its preparation method and application

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Embodiment 1

[0036] 1) Synthesis of cadmium telluride quantum dots: under an inert gas atmosphere (argon), tellurium powder and NaBH 4 Stir in water, then add CdCl at pH 10 2 React with the mixed solution of TGA to control the Cd in the reaction product 2+ :TGA:Te 2- The molar ratio is 1:2.5:0.5;

[0037] 2) Synthesis of amino-modified carbon quantum dots: Centrifuge the citric acid aqueous solution with a concentration of 0.04g / ml after high temperature treatment, take the supernatant, and mix the supernatant with ammonium hydroxide at a volume ratio of 1:48 And carry out high-temperature treatment, add acetone after cooling down to room temperature, and then centrifuge, take the supernatant and place it in a vacuum drying oven to volatilize the acetone;

[0038] 3) Doped SiO 2 The synthesis of the blue fluorescent carbon quantum dot of nanoparticle: after step 2) gain is mixed with the chitosan solution of 0.5% (W / V), join in the mixed solution of cyclohexane, Triton X-100, n-hexanol...

Embodiment 2

[0041] 1) Synthesis of cadmium telluride quantum dots: under an inert gas atmosphere (argon), tellurium powder and NaBH 4 Stir in water, then add CdCl at pH 10 2 React with the mixed solution of TGA to control the Cd in the reaction product 2+ :TGA:Te 2- The molar ratio is 1:2.5:0.5;

[0042] 2) Synthesis of amino-modified carbon quantum dots: Centrifuge the citric acid aqueous solution with a concentration of 0.04g / ml after high temperature treatment, take the supernatant, and mix the supernatant with ammonium hydroxide at a volume ratio of 1:36 And carry out high-temperature treatment, add acetone after cooling down to room temperature, and then centrifuge, take the supernatant and place it in a vacuum drying oven to volatilize the acetone;

[0043] 3) Doped SiO 2 The synthesis of the blue fluorescent carbon quantum dot of nanoparticle: after step 2) gain is mixed with the chitosan solution of 0.5% (W / V), join in the mixed solution of cyclohexane, Triton X-100, n-hexanol...

Embodiment 3

[0046] 1) Synthesis of cadmium telluride quantum dots: under an inert gas atmosphere (argon), tellurium powder and NaBH 4 Stir in water, then add CdCl at pH 10 2 React with the mixed solution of TGA to control the Cd in the reaction product 2+ :TGA:Te 2- The molar ratio is 1:2.5:0.5;

[0047] 2) Synthesis of amino-modified carbon quantum dots: centrifuge the citric acid aqueous solution with a concentration of 0.04g / ml after high temperature treatment, take the supernatant, and mix the supernatant with ammonium hydroxide at a volume ratio of 1:50 And carry out high-temperature treatment, add acetone after cooling down to room temperature, and then centrifuge, take the supernatant and place it in a vacuum drying oven to volatilize the acetone;

[0048] 3) Doped SiO 2 The synthesis of the blue fluorescent carbon quantum dot of nanoparticle: after step 2) gain is mixed with the chitosan solution of 0.5% (W / V), join in the mixed solution of cyclohexane, Triton X-100, n-hexanol...

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Abstract

The invention discloses a preparation method of a ratiometric fluorescent probe for bivalent copper ions. The preparation method comprises the following steps: 1) synthetizing cadmium telluride quantum dots: controlling the molar ratio of Cd2<+>:TGA:Te2<-> in a reaction product to be 1:2.5:0.5; 2) synthetizing amino-modified carbon quantum dots; 3) synthetizing blue-fluorescence carbon quantum dots doped with SiO2 nanoparticles: during synthesis, the volume ratio of the amino-modified carbon quantum dots obtained in the step 2), a chitosan solution of which the W / V is 0.5 percent, cyclohexane, Triton X-100, hexyl alcohol, ammonia water, TEOS and APTES is 5:1:750:177:180:5:6:3; 4) dispersing the cadmium telluride quantum dots obtained in the step 1) and the blue-fluorescence carbon quantum dots obtained in the step 3) in an MES buffer solution, adding a mixed solution of EDC and NHS, carrying out stirring, centrifugation and cleaning to obtain the probe. The probe prepared according to the method comprises nano particles containing the cadmium telluride quantum dots and the amino-modified carbon quantum dots, and has nucleus-satellite hybrid spherical structures. The invention further discloses application of the ratiometric fluorescent probe to detection of Cu2<+>. The ratiometric fluorescent probe has the advantages that the detection limit is very excellent, namely only 1.0*10<7>M; the preparation is simple; no expensive equipment is required.

Description

technical field [0001] The invention belongs to the field of detection of divalent copper ions, and in particular relates to a ratio fluorescent probe of divalent copper ions and its preparation method and application. Background technique [0002] Divalent copper ion is the third largest transition metal ion in the human body and is essential for many biological reactions. However, if the content of divalent copper ions is too low or too high, biological systems will be adversely affected. Therefore, it is of great significance to develop a specific and sensitive detection method for divalent copper ions. Conventional methods in the prior art have disadvantages such as complex operation, expensive equipment and a large number of test samples. [0003] With the deepening of research, the ratiometric fluorescence probe has been paid more and more attention. Zhu et al prepared ratiometric fluorescent probes based on carbon quantum dots (A.Zhu, Q.Qu, X.Shao, B.Kong and Y.Tia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88B82Y20/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/883G01N21/6428
Inventor 饶含兵王妍媖鲁智伟刘伟姚瑶葛宏伟何桦王显祥曾宪垠
Owner SICHUAN AGRI UNIV
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