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Anti-H-FABP antibody labeled by colloidal selenium, detection card, and preparation method thereof

A colloidal selenium and antibody technology, which is applied in the field of biological immunochromatography detection, can solve the problem of high cost, and achieve the effect of low cost, short detection time and cost economy

Inactive Publication Date: 2019-01-29
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of colloidal selenium-labeled anti-H-FABP antibody, thus solve the high cost of the existing colloidal gold-labeled anti-H-FABP antibody, which is not applicable to the characteristics of the home self-examination of H-FABP

Method used

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  • Anti-H-FABP antibody labeled by colloidal selenium, detection card, and preparation method thereof
  • Anti-H-FABP antibody labeled by colloidal selenium, detection card, and preparation method thereof
  • Anti-H-FABP antibody labeled by colloidal selenium, detection card, and preparation method thereof

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Effect test

Embodiment 1

[0042] The colloidal selenium-labeled anti-H-FABP antibody of this embodiment was prepared using the following steps:

[0043] 1) Put 16mL of ultrapure water into a 50mL small beaker, add 1mL of PEG solution with a concentration of 1mol / L, stir at room temperature for 20min to disperse evenly, and obtain a dilute PEG solution;

[0044] 2) Mix 1 mL of SDS solution with a concentration of 0.1 mol / L and PEG dilute solution, stir at room temperature for 20 minutes to disperse evenly, and obtain a template solution;

[0045] 3) Mix 1 mL of selenous acid solution with a concentration of 0.04 mol / L and the template solution, stir at room temperature for 20 minutes to disperse evenly, and obtain the selenous acid template solution;

[0046] 4) Mix 1 mL of vitamin C with a concentration of 0.32 mol / L and a selenous acid template solution, stir at room temperature for 20 minutes to disperse evenly, and obtain colloidal selenium;

[0047] 5) Take 1mL of colloidal selenium, add 8μL of po...

Embodiment 2

[0057] The colloidal selenium-labeled anti-H-FABP antibody of this embodiment was prepared using the following steps:

[0058] 1) Put 16mL of ultrapure water into a 50mL small beaker, add 1mL of PEG solution with a concentration of 1mol / L, stir at room temperature for 20min to disperse evenly, and obtain a dilute PEG solution;

[0059] 2) Mix 1 mL of SDS solution with a concentration of 0.2 mol / L and PEG dilute solution, stir at room temperature for 20 minutes to disperse evenly, and obtain a template solution;

[0060] 3) Mix 1 mL of selenous acid solution with a concentration of 0.04 mol / L and the template solution, stir at room temperature for 20 minutes to disperse evenly, and obtain the selenous acid template solution;

[0061] 4) Mix 1 mL of vitamin C with a concentration of 0.32 mol / L and a selenous acid template solution, stir at room temperature for 20 minutes to disperse evenly, and obtain colloidal selenium;

[0062] 5) Take 1 mL of colloidal selenium, add 1 mol / L ...

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Abstract

The invention relates to an anti-H-FABP antibody labeled by colloidal selenium, a detection card, and a preparation method thereof. The anti-H-FABP antibody contains colloidal selenium particles and the anti-H-FABP antibody combined therewith. In the invention, the anti-H-FABP antibody labeled by colloidal selenium is used as a detection antibody on the H-FABP detection card, wherein the antibodyis low in cost; a test proves that the detection card is strong in detection specificity and short in detection time, so that the detection card can be extensively used for home self-detection of theH-FABP for myocardial infarction patients in early stage.

Description

technical field [0001] The invention belongs to the technical field of biological immunochromatography detection methods, and in particular relates to a colloidal selenium-labeled anti-H-FABP antibody, a detection card and a preparation method thereof. Background technique [0002] Acute myocardial infarction (AMI) is myocardial necrosis caused by unstable ischemic syndrome. Myocardial necrosis is an irreversible damage that occurs in 85 percent of hearts within two hours of a heart attack, and delayed medical treatment increases the likelihood of death. Then, it is of great clinical value to make an early and rapid diagnosis of acute myocardial infarction. At present, the clinical diagnosis of AMI mainly relies on the detection of cardiac injury markers, serum biochemical indicators, electrocardiogram, imaging (magnetic resonance, ultrasound, etc.) and clinical symptoms. However, most of these inspection methods require expensive detection equipment, complicated operation...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577C07K16/18
CPCG01N33/6893C07K16/18G01N33/577G01N2800/324
Inventor 马远方王志增陶宁亚郭亚飞周倩文王耀辉张军刘广超李淑莲
Owner HENAN UNIVERSITY
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