Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting benzoic acid content in Kushen compound salicylic acid powder

A detection method and technology of benzoic acid, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to reflect the content of each component, difficult to effectively control the content of drugs, etc., and achieve easy control, good durability, and strong detection specificity. Effect

Inactive Publication Date: 2015-11-04
CHENGDU JIUZHITANG JINDING PHARMA
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The content determination of the current international drug standards of the State Drug Administration, the Chinese patent medicine Zuguangsan standard uses the volumetric titration method to measure the total acid content of benzoic acid, salicylic acid and boric acid in Zuguangsan, which cannot reflect the content of each component and is difficult to effectively control drug content
At present, there is no patent document in China to report the content of benzoic acid in Zuguangsan by high performance liquid chromatography. Only Yu Xiaoping published an article about the determination of benzoic acid in Zuguangsan in "Journal of Pharmaceutical Analysis" in 2005. This method adopts methanol-0.1mol / L sodium dihydrogen phosphate solution as the mobile phase, the detection wavelength is 240nm, and water is added to extract benzoic acid. It is well known that the sufficient light dispersion is not completely dissolved in water, and when the sufficient light dispersion is dissolved, most of the sufficient light dispersion components It sinks to the bottom of the water, and benzoic acid is slightly soluble in water, so this method has certain limitations in detecting the content of benzoic acid in foot light scattering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting benzoic acid content in Kushen compound salicylic acid powder
  • Method for detecting benzoic acid content in Kushen compound salicylic acid powder
  • Method for detecting benzoic acid content in Kushen compound salicylic acid powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] [embodiment 1] a kind of detection method of benzoic acid content in Zuguangsan, adopts high performance liquid chromatography detection, comprises the following steps:

[0025] S1. Preparation of reference substance solution and test solution:

[0026] (1) Preparation of reference substance solution: get an appropriate amount of analytically pure benzoic acid, accurately weighed, add dehydrated alcohol to make a solution containing 1.2mg in every 1ml, as reference substance solution;

[0027] (2) Preparation of the test solution: take 0.5g of Zuguangsan, accurately weigh it, put it in a 50ml measuring bottle, add 40ml of absolute ethanol, seal it tightly, process it ultrasonically for 3min, let it cool, and dilute to the mark with absolute ethanol , shake well, filter, and take the continued filtrate as the test solution;

[0028] S2. Accurately measure 10 μl each of the test solution and the reference solution, inject it into a liquid chromatograph, and measure it. D...

Embodiment 2

[0030] [embodiment 2] a kind of detection method of benzoic acid content in Zuguangsan, adopts high performance liquid chromatography detection, comprises the following steps:

[0031] S1. Preparation of reference substance solution and test solution:

[0032] (1) Preparation of reference substance solution: get an appropriate amount of analytically pure benzoic acid, accurately weighed, add dehydrated alcohol to make a solution containing 1.2mg in every 1ml, as reference substance solution;

[0033] (2) Preparation of the test solution: take 0.5g of Zuguangsan, accurately weigh it, put it in a 50ml measuring bottle, add 40ml of absolute ethanol, seal it tightly, treat it with ultrasound for 4min, let it cool, and dilute to the mark with absolute ethanol , shake well, filter, and take the continued filtrate as the test solution;

[0034] S2. Accurately measure 10 μl each of the test solution and the reference solution, inject it into a liquid chromatograph, and measure it. Du...

Embodiment 3

[0036] [embodiment 3] a kind of detection method of benzoic acid content in Zuguangsan, adopts high performance liquid chromatography detection, comprises the following steps:

[0037] S1. Preparation of reference substance solution and test solution:

[0038] (1) Preparation of reference substance solution: get an appropriate amount of analytically pure benzoic acid, accurately weighed, add dehydrated alcohol to make a solution containing 1.2mg in every 1ml, as reference substance solution;

[0039] (2) Preparation of the test solution: take 0.5g of Zuguangsan, accurately weigh it, put it in a 50ml measuring bottle, add 40ml of absolute ethanol, seal it tightly, ultrasonically treat it for 5min, let it cool, and dilute to the mark with absolute ethanol , shake well, filter, and take the continued filtrate as the test solution;

[0040] S2. Accurately measure 10 μl each of the test solution and the reference solution, inject it into a liquid chromatograph, and measure it. Dur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting benzoic acid content in Kushen compound salicylic acid powder. The method uses high performance liquid chromatography and comprises the steps of: S1, preparation of a reference solution and a test solution comprises (1) preparation of the reference solution by taking a proper amount of analytically pure benzoic acid, accurately weighing, adding absolute ethyl alcohol to produce a solution as the reference solution containing 1.2 mg per 1 ml; (2) preparation of the test solution comprises taking 0.5g of Kushen compound salicylic acid powder, accurately weighing, adding 50ml into a volumetric flask, adding 40ml of absolute ethyl alcohol, sealing with plug, conducting ultrasonic treatment for 3-7 min, cooling, diluting with absolute ethyl alcohol to the mark, shaking evenly, filtering, and taking the filtrate as the test solution; and S2, detection: taking precisely 10 mul of the test solution and 10 mul of the reference solution, injecting into the liquid chromatograph, and measuring by using 0.03-0.07mol / L potassium dihydrogen phosphate solution-methanol as the mobile phase. The detection method provided by the invention is simple and easy to operation; the extraction of benzoic acid in the Kushen compound salicylic acid powder is thorough; and the detection result is accurate and reliable.

Description

technical field [0001] The invention relates to a method for detecting the content of benzoic acid in Zuguangsan, belonging to the field of drug detection. Background technique [0002] Zuguangsan is a light yellow-brown to light red-brown powder, and its main components are salicylic acid, benzoic acid, boric acid, and flavescens. The auxiliary material is talcum powder. Zuguangsan has the effects of clearing away heat and dampness, killing insects and astringing sweat. It is mainly used for keratotic tinea pedis and fetid sweat caused by betting on damp heat. [0003] Benzoic acid is a scaly or needle-like crystal with the smell of benzene or formaldehyde. It sublimates rapidly at 100°C, and its vapor is highly irritating, which can easily cause coughing after inhalation. Benzoic acid is slightly soluble in water and easily soluble in organic solvents such as ethanol and ether. Benzoic acid is a weak acid, stronger than fatty acids. Their chemical properties are simila...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 张宁李欧曾嵘陈曦蒋丽郑飞
Owner CHENGDU JIUZHITANG JINDING PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com