Primer pair, probe and kit for detecting human VDR, GC, LRP5 and SLC30A8 gene polymorphism

Abstract

The invention discloses a primer pair, a probe and a kit for detecting human VDR, GC, LRP5 and SLC30A8 gene polymorphism, and in particular a primer pair, a probe and a kit for detecting the gene polymorphism with the adoption of a multiple-asymmetrical fluorescent PCR probe melting curve method, wherein nucleotide sequences of upstream primers and downstream primers, which are used for amplifyinghuman VDR, GC, LRP5 and SLC30A8 genes, are sequentially shown SEQ ID NO:1-SEQ ID NO:8. The detection kit provided by the invention, which is free from complex experimental operations, is rapid and convenient; through complete closed-tube operations, cross pollution is prevented; and the kit is low in cost, high in sensitivity and strong in specificity, and the kit is applicable to large-scale promotion and application.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a primer pair, a probe and a detection kit for detecting human VDR, GC, LRP5 and SLC30A8 gene polymorphisms by the melting curve method of multiple asymmetric fluorescent PCR probes. Background technique [0002] With the improvement of people's living standards, parents often blindly supplement their children with various "supplements" for the healthy growth of their children. As a result, not only did not achieve any expected effect, but it may also cause the ratio of nutrients in the child's body to be out of balance, resulting in the loss of certain nutrients. Excess or deficiency, which brings negative results, such as precocious puberty and so on. In fact, what should be supplemented for children should be nutrients that are easily lacking due to various reasons. This requires parents to understand from the source which nutrients their children will lack and w...

AI Technical Summary

Problems solved by technology

Different methods have their own limitations. For example, RDB is a heterogeneous mutation detection method, which requires subsequent processing of PCR products. There are many operating steps, which are easy to cause pollution, and the interpretation of results is easily affected by subjective factors; the cost of gene chips Expensive and difficult to popularize; the detection rate of SSCP is easily affected by electrophoresis conditions (such as pH value, ionic strength and temperature, etc.), among which the influence of temperature is the most sensitive, and the electrophoresis temperature needs to be strictly controlled; fluorescent quantitative PCR requires fluorescently labeled specific probes , a probe can only detect one type of gene mutation, so the cost of detection reagents is high, the types of mutations detected are relatively complex, and the operation is relatively complicated; HRM cannot easily use multiple modes to simultaneously type several different fragments at the same time, mutations, etc.

Images