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Method for constructing nanocellulose paper-based biosensor

A technology of nanocellulose and biosensors, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of rough surface, poor detection efficiency and accuracy, and large internal pores, so as to achieve smooth surface, increase added value, and high The effect of light transmittance

Active Publication Date: 2018-12-04
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problem of poor detection efficiency and accuracy caused by the large internal pores and rough surface of existing paper-based biosensors, and provides a method for constructing nanocellulose paper-based biosensors

Method used

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  • Method for constructing nanocellulose paper-based biosensor
  • Method for constructing nanocellulose paper-based biosensor
  • Method for constructing nanocellulose paper-based biosensor

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specific Embodiment approach 1

[0050] Specific embodiment one: The construction method of nanocellulose paper-based biosensor in this embodiment includes the following steps:

[0051] One, the preparation of nanocellulose:

[0052] The cellulose raw material is sequentially subjected to extraction treatment and delignification treatment to obtain hemicellulose, and then mechanical pretreatment, chemical mechanical mixing pretreatment or biomechanical mixing pretreatment is performed to obtain hemicellulose-containing nanocellulose aqueous solution;

[0053] Described mechanical pretreatment, concrete operation steps are as follows:

[0054] ① Adding deionized water to the helium cellulose until the mass fraction of the helium cellulose is 0.1% to 0.3%;

[0055] ② Then use a 600bar high-pressure homogeneous mechanical treatment for 30-40 minutes to obtain a nano-cellulose aqueous dispersion containing hemicellulose, wherein the hemicellulose accounts for 20%-30% of the total cellulose mass;

[0056] The sp...

specific Embodiment approach 2

[0075] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the specific steps of the extraction process described in step one are:

[0076] Crush the cellulose raw material into 90-120 mesh powder, and then carry out extraction treatment with benzyl alcohol for 10-12 hours. The benzyl alcohol is a mixture of toluene and absolute ethanol in a volume ratio of 2:1. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0077] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the specific steps of delignification treatment described in step one are:

[0078] ① Immerse the extracted cellulose powder in a sodium chlorite solution with a mass concentration of 1% to 1.2%, adjust the pH value of the solution to 4 to 5 with glacial acetic acid, and then magnetically place it in a constant temperature water bath at 75 to 80°C. Heat and stir for 1 to 1.5 hours;

[0079] ②Immerse the cellulose powder obtained in step ① into a sodium chlorite solution with a mass concentration of 1% to 1.2%, adjust the pH value of the solution to 4 to 5 with glacial acetic acid, and then magnetically heat it in a constant temperature water bath at 75 to 80°C Stir for 1~1.5h;

[0080] ③ Repeat step ② for 5-6 times to basically remove the lignin, then filter and wash the obtained liquid with a Buchner funnel until the filtrate is neutral, and finally obtain hedophilic cell...

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Abstract

The invention relates to a method for constructing a biosensor, specifically to a method for constructing a nanocellulose paper-based biosensor. The objective of the invention to overcome the problemsof low detection efficiency and poor detection accuracy of conventional paper-based biosensors caused by great internal porosity and rough surfaces in the prior art. The method comprises the following steps: 1, peparation of nanocellulose: sequentially subjecting a cellulose raw material to extraction and delignification so as to obtain total cellulose, and then carrying out mechanical pretreatment, chemical-mechanical mixed pretreatment or biomechanical mixed pretreatment to obtain aqueous hemicellulose-containing nanocellulose liquid; 2, preparation of nanopaper; and 3, construction of thenanopaper-based biosensor. Detection results show that the nanopaper prepared in the invention has no obvious voids and micron-order roughness, wherein the porosity of the nanopaper is more than 1%, and the roughness of the nanopaper is less than 1 [mu]m. The method provided by the invention is applied to the field of biosensors.

Description

technical field [0001] The invention relates to a construction method of a biosensor. Background technique [0002] The 21st century is the century of information science and life science. Biosensor is a high-tech product of life science and information technology integration and multidisciplinary interpenetration. Biosensing technology is a kind of bioanalysis technology with strong specificity and high sensitivity, which can realize the detection and analysis of biochemical substance reagents. [0003] The development of biosensors (Biosensor) has experienced three generations of enzyme electrodes, mediator enzyme electrodes, and electrochemical biosensors. It has shown unique advantages in the fields of food safety, environmental monitoring, and health diagnosis. However, traditional biosensors are based on plastic, glass, etc., which have disadvantages such as non-renewable, difficult to degrade, and inconvenient to carry. [0004] For this reason, various biosensors b...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3278
Inventor 李永峰董晓英
Owner SHANDONG AGRICULTURAL UNIVERSITY
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