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Method for extracting haw leaf general flavone material for injection using polyamide column

A technology of hawthorn leaves and total flavonoids is applied in the directions of plant raw materials, pharmaceutical formulations, medical preparations containing active ingredients, etc. Residue reduction and protection of natural properties

Inactive Publication Date: 2006-10-04
SHANGHAI YOUSEEN NEW MEDICINE EXPLOITION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been found through research that the tannin in the total flavonoids of hawthorn leaves purified by the method of extracting the total flavonoids of hawthorn leaves with macroporous adsorption resin is not easy to remove, which will affect the purity and curative effect of the extract, and is especially not suitable for use as injections; High-concentration pickling and alkali washing, so that some kind of acid-base reaction cannot be completely avoided
There are also many other patented technologies that use n-butanol to extract before applying various adsorption columns, but n-butanol is very expensive and pollutes heavily. It remains in the drug extract and cannot be completely removed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take dried hawthorn leaves, soak them with 10 times the amount of crude drug for three times at 60°C for 2 hours each time, combine the extracts, filter, and concentrate the filtrate under reduced pressure to contain crude drug 0.5g / ml, and add ethanol until the alcohol content reaches 60 %, let stand and refrigerate for 12 hours. Aspirate the supernatant, concentrate under reduced pressure until it has no alcohol taste, add deionized water to 0.2-0.5g / ml of crude drug, let stand, refrigerate for 12 hours, filter, and filter the filtrate on a polyamide chromatography column and wash with deionized water to The α-naphthol concentrated sulfuric acid reaction is negative, the water washing solution is discarded, and then 30% ethanol is used to elute until there is no hydrochloric acid-magnesium powder reaction. The ethanol eluate was collected, concentrated under reduced pressure to a thick paste, and dried under reduced pressure in a vacuum drying cabinet at 60° C. to a powde...

Embodiment 2

[0014] Take the dried hawthorn leaves and warm them with 15 times the amount of crude drug for three times at 70°C for 1.5 hours each time. Combine the infusions and filter. The filtrate is concentrated under reduced pressure to 0.7g / ml crude drug, and ethanol is added until the alcohol content reaches 70. %, let stand and refrigerate for 24 hours. Aspirate the supernatant, concentrate under reduced pressure until it has no alcohol taste, add deionized water to 0.3g / ml of crude drug, let stand, refrigerate for 24 hours, filter, and filter the filtrate on a polyamide chromatography column and wash with deionized water to α- The reaction of naphthol concentrated sulfuric acid is negative, the water washing solution is discarded, and then 30% ethanol is used to elute until the reaction of hydrochloric acid-magnesium powder is free. The ethanol eluate was collected, concentrated under reduced pressure to a thick paste, and dried under reduced pressure in a vacuum drying cabinet at 60°...

Embodiment 3

[0016] Take 10 kg of dried hawthorn leaves and soak them with 17 times the amount of crude drug at 90°C for three times for 2 hours each time. Combine the infusions and filter. The filtrate is concentrated under reduced pressure to 1.5g / ml crude drug, and ethanol is added until the alcohol content reaches 90%, let stand and refrigerate for 24 hours. Aspirate the supernatant and concentrate under reduced pressure until there is no alcohol, add deionized water to 40L of crude drug, let stand, refrigerate for 24 hours, filter, and filter the filtrate on a polyamide chromatography column, wash with deionized water to a concentration of α-naphthol The sulfuric acid reaction is negative, discard the washing liquid, and then eluted with 50% ethanol until the hydrochloric acid-magnesium powder reaction stops. The ethanol eluate was collected, concentrated under reduced pressure to a thick paste, and dried under reduced pressure in a vacuum drying cabinet at 60° C. to a powder, to obtain 1...

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Abstract

The invention provides a process for preparing extract of haw flavones for injection, which is characterized by simplified procedure, low chemical residual, light contamination and low cost. The extracted haw flavones can achieve a content meeting national standard for injections.

Description

Technical field [0001] The invention relates to a process technology for extracting total flavonoids from hawthorn leaves, especially a method using polyamide chromatography, which belongs to the technical field of traditional Chinese medicine pharmacy. technical background [0002] Hawthorn is the dried leaf of Crataegus pinnatifida Bge.Var.major N.E.Br., a rosaceous plant. It has the functions of digesting food, invigorating the stomach, promoting qi and removing blood stasis. Zhang Xichun said that it "good blood can be divided into the main medicine for removing blood stasis." Clinical use of hawthorn to treat coronary heart disease and prevent cardiovascular system diseases has achieved good results. In recent years, studies have found that hawthorn leaves also have a good effect on preventing and treating cardiovascular and cerebrovascular diseases, and can prevent coronary heart disease and prevent ischemia-reperfusion injury. The knowledge of hawthorn leaf extract is that...

Claims

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Application Information

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IPC IPC(8): A61K36/734A61K9/08A61P9/10A61P7/02
Inventor 玄振玉
Owner SHANGHAI YOUSEEN NEW MEDICINE EXPLOITION
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