220results about How to "Simplified extraction steps" patented technology

The invention discloses a printed and handwritten mixed text line extraction system, which comprises text block area preprocessing and text line extraction. Through the text block area preprocessing process firstly, a document image containing a printed or handwritten or mixed text is subjected to skew correction, and a text block area is acquired through layout analysis; the text block area is then subjected to connected domain clustering, the belonging text line label is distributed, touching character pixels are subjected to clustering segmentation and the belonging text line label is redistributed; and finally, through the text line label, extraction of a document image text line is realized. The defects of missing extracted stroke information or redundant stroke information introduction caused by inclined touching of the text line in a handwritten text or a printed and handwritten mixed text in the prior art can be overcome, the pixel controllability during the text line extraction process is improved, and the purpose of quickly and efficiently extracting the text line is thus achieved.

The invention discloses a lane line extraction method, apparatus and system, relates to the technical field of traffic, and mainly aims to improve lane line extraction precision and efficiency. The method comprises the steps of collecting a measureable real scene road image; by analyzing the measureable real scene road image, constructing a grid dynamic window-based lane identification region of interest, and performing lane line edge detection on the lane identification region of interest; and extracting lane lines according to lane line information obtained by detection and a lane line extraction model subjected to line fitting correction in a preset lane. The lane line extraction method, apparatus and system is suitable for measureable real scene road image-based lane line extraction.

The present invention is one Streptomyces strain and the process of utilizing the Streptomyces strain in converting ferulic acid to produce high concentration vanillin. The Streptomyces strain is Streptomyces sp.V-1 in the preservation number of CCTCC M 206065. In the GY converting culture medium with the Streptomyces strain, ferulic acid may be converted to produce high concentration vanillin fermenting liquid. Adding macroporous adsorption resin DM11 into the converting culture medium can increase the concentration of vanillin fermenting liquid greatly. The present invention has the advantages of less environmental pollution, high product concentration, less side products, short production period, low cost, etc.

The invention discloses a method for extracting the width of river channels within a basin by using a digital elevation model. The method comprises the following main steps: the digital elevation model (DEM) data of the basin are extracted; the slope grids, river channel grids and confluence accumulative grids of the basin are calculated according to the digital elevation model (DEM) data of the basin; near-river channel slope grids are extracted by using the slope grids and the river channel grids; near-river channel confluence accumulative grids are extracted by using the confluence accumulative grids and the river channel grids; moving average calculation is performed on the basis of the near-river channel slope grids, so that moving averaged near-river channel slope grids can be obtained; and the width of the river channels within the basin is extracted on the basis of the near-river channel confluence accumulative grids and the moving averaged near-river channel slope grids. The method of the invention has the advantages of stable and reliable data sources, high calculation efficiency, objective and reasonable results and the like. With the method adopted, the rapid calculation and extraction of the width of the river channels within the basin can be benefitted.

The invention discloses a method for simultaneously extracting and separating solanesol and nicotine from a tobacco. The method comprises the following steps of: (1) extracting the solanesol and the nicotine at the same time; (2) extracting and separating; (3) separating and purifying the solanesol; (4) separating and purifying the nicotine. Because the nicotine is separated and extracted and the solanesol is saponified and extracted at the same time, the extraction steps are simplified greatly, and the consumption of time and solvents is saved. Compared with the prior art, the method disclosed by the invention is efficient, ensures that more products are extracted and less waste liquid is discharged, is simple in step and is an environmentally-friendly production technology.

A device and method for extracting micro-plastic in settlings are provided. The device includes a filter drum, a liquid storage tank, a motor, a pedestal, a screen sieve and a transmission shaft. The filter drum is mounted in the liquid storage tank. The upper port of the filter drum is provided with the screen sieve. The liquid storage tank and the motor are mounted on the pedestal. The bottom of the liquid storage tank is provided with a drainage hole communicated with the filter drum. The motor is connected to one end of the transmission shaft through a rotating shaft, and the other end of the transmission shaft passes through the drainage hole and is connected to the bottom of the filter drum. The device and the method are advantageous in that (1) dispersion effects of muddy settlings and elution effects of a sample are obviously improved; (2) the recovery rate of the sample is increased, (3) the extraction efficiency is greatly increased, (4) extraction time is shortened and (5) labor is saved.

The invention discloses a production method of white zein. According to the method, corn protein powder serves as raw materials, starch removing treatment is carried out by means of adding of alpha-amylase and glucoamylase, ethanol is added to carry out degreasing and preliminary decoloration treatment, filteration is achieved, filter residues are added to an ethanol water solution to extract, a zein extracting solution is obtained through centrifugal separation, the zein extracting solution is added to a decolorising agent to carry out secondary decoloration treatment, and at last white zein end products are obtained through concentration and drying. According to the production method of the white zein, decontamination pretreatment is carried out for the raw materials, and the concentration and the drying are directly carried out after the decoloration treatment for the extracting solution, so the production method has the advantages of being high in yield, high in purity, low in production cost, and efficient in decoloration, the application range of zein powder is significantly expanded, and market developing of the zein powder is benefited.

The invention provides a method for separating and extracting Sirolimus from fermentation liquid. The extraction process comprises the following steps of: preparing Sirolimus fermentation mycelia; performing extraction and decoloration; performing high-speed countercurrent chromatography separation; and drying. In the method, the multi-chromatography steps in the traditional extraction process are replaced by countercurrent chromatography separation technology; extraction is combined with countercurrent; and a Rapamycin product with purity of near 90 percent can be obtained by one-step countercurrent separation. Compared with the relatively complex traditional Rapamycin extraction process, the fermentation mycelia crude extraction and high-speed countercurrent chromatography method has the advantages of simple separation route, contribution to amplification of the process and suitability for quickly separating and preparing high-additional-value Rapamycin monomers from the fermentation liquid in large scale. The method for separating and extracting the Sirolimus from the fermentation liquid has the advantages of high yield, simple extraction steps, solvent saving and the like, and can be applied to industrial actual amplified production.

The present invention discloses a strain of Bacillus methylotrophicus VJ4-1, and a method for producing natural vanillin by ferulic acid biotransformation with the Bacillus methylotrophicus VJ4-1, wherein a name of the strain is Bacillus methylotrophicus VJ4-1, the strain is preserved in the China center for type culture collection (CCTCC) in Wuhan University on January 16, 2012, and a preservation number is CCTCC NO:M2012004. The method of the present invention has advantages of high yield, simple process, mild reaction conditions, less environmental pollution, high product concentration, simple extraction step, clean production, environmentally friendly products, safety, reliability, wide main raw material source, low cost, and natural property, and has an industrial production prospect.

The invention discloses a mountain torrent rapid early warning and forecasting method based on multi-dimensional rainfall characteristic space optimal decision. The method comprises: firstly, calculating initial soil water saturation S of a successive historical rainfall flood process based on a three-layer evapotranspiration principle; secondly, counting the rainfall P of the successive historical rainfall flood process; thirdly, quantifying a rainfall space distribution characteristic K of a successive historical rainfall flood process by combining drainage basin DEM data; calculating a landform unit line of the drainage basin based on the DEM data; taking P, K and S as mutually orthogonal coordinate axes to construct a multi-dimensional rainfall characteristic space coordinate system, establishing a rainfall data point set, dividing the corresponding rainfall data point set into two types of an ultra-alarm subset A and a non-ultra-alarm subset B according to whether the flood process is over-alarm or not, and solving an optimal classification decision equation between the two types of subsets by combining an SVM principle; and if early warning is needed, calculating the runoff process at the outlet of the drainage basin by taking the rainfall as the input of a landform unit line, and carrying out real-time flood forecasting.

The invention discloses a method for extracting DNA (Deoxyribonucleic Acid) of a rice leaf blade or a rice seed rapidly and efficiently by using a 96 deep-hole plate and a mixer mill, relating to the field of biology. The method is a rapid and effective plant DNA extraction method, the whole extraction course is free from single-shotgun operation but undergoes stranded-shotgun operation completely, and in addition, serial numbers do not need to be written, so that a rapid high-flux extraction method is realized. The method can be applied to rice molecular breeding, purity identification, seed authenticity detection, gene cloning and the like.

The invention discloses a system for inducing foreign genes to express in gram-negative bacteria and application of the system. The invention provides a method for expressing the foreign genes in the gram-negative bacteria. According to the method, a regulating and control element comprising a repressor protein expression box and an RNA (ribonucleic acid) polymerase expression box is used for regulating and controlling the expression of a foreign gene expression box in the gram-negative bacteria. Experiments prove that the system for inducing the foreign genes to effectively express in the gram-negative bacteria provided by the invention consists of two parts including specific polymerase and a corresponding promoter; the polymerase, the promoter and driven foreign genes are induced to express in the gram-negative bacteria; the efficient mass expression of the foreign genes can be realized; the important significance of simplifying the extraction step, improving the protein expression quantity and reducing the production cost are realized in industrial production; wide application prospects are realized; wide commercial utilization values are realized.

The invention provides a method for measuring sixteen polycyclic aromatic hydrocarbons in cigarette liquid of an electronic cigarette. The method is characterized in that a small amount of water is added to a cigarette liquid sample of the electronic cigarette, a mixture vibrates evenly, so that the mixture is fully dispersed in a kieselguhr solid phase supporting liquid-liquid extraction column, cyclohexane is used for extraction, and after extraction liquid is concentrated, the sixteen polycyclic aromatic hydrocarbons in the cigarette liquid of the electronic cigarette are measured through a GC-MS. Compared with a commonly-used polycyclic aromatic hydrocarbon analysis method, the method for measuring the sixteen polycyclic aromatic hydrocarbons has the advantages that sample pretreatment is greatly simplified, the use amount of organic solvents is saved, experiment repeatability is good, the experiment recovery rate is high, and the method is suitable for treating samples automatically on a large scale.

The invention provides a method for extracting Sargassum furiforme polysaccharides, which has the advantages of simple operation steps, low energy and material consumption, higher polysaccharide content and higher purity. The method comprises the following steps: (1) drying Sargassum furiforme, pulverizing the Sargassum furiforme into powder, and passing the powder through a 40-200-mesh screen; (2) thoroughly mixing the Sargassum furiforme alga powder obtained in the step (1) with 95% ethanol, and carrying out reflux degreasing; (3) recycling the degreased alga powder in the step (2), adding distilled water, filtering through bolting silk several times, collecting the filtrates, merging the filtrates, and concentrating under reduced pressure to obtain a concentrated solution; (4) adding 95% ethanol into the concentrated solution in the step (3), carrying out alcohol precipitation, centrifugating, collecting the precipitate, adding distilled water, and stirring until the solid is completely dissolved; and (5) carrying out freeze vacuum drying on the polysaccharide solution in the step (4) to obtain the polysaccharide product.

The invention discloses Bacillus pumilis and its application of biotransformation of iso-eugenol into natural vanillin. The name of bacterial stripe is Bacillus pumilus S-1, preserved in China Center for Type Culture Collection on 3rd, January, 2006 and its serial number is CCTCC M 205165. The procedure of transforming iso-eugenol into natural vanillin includes: (1)cultivation, (2)preparation of seed culture fluid, (3)culture of transforming cell, (4) transformation.The invention exists in field of biology and technology. It can improve the transformating rate of vanillin with high concentration, solving problems of low concentration and low yield of vanillin by biological method.

The invention relates to the technical field of biologics, in particular to a method for extracting a toxin protein which causes vascular permeability increase from white cyanea tentacles. The method comprises the following steps: unfreezing fresh white cyanea tentacles which are frozen at a super-low temperature at a low temperature, washing by a small quantity of buffer solution, collecting the buffer solution, centrifuging to obtain a supernatant and using the supernatant as cyanea crude toxin, and sequentially separating by an ion chromatographic column and a gel chromatographic column to obtain the toxin protein CnP45 which causes vascular permeability increase, wherein the molecular weight of the toxin protein is 45kD. The extracting process of the jellyfish toxin is simplified, the interference of impurity proteins is reduced, and an important method instruction is provided for the further separation and purification of jellyfish toxin ingredients.

The invention discloses a nucleic acid extraction kit comprising a nucleic acid binding solution and a magnetic adsorbent. The nucleic acid binding solution comprises polyethylene glycol and sodium chloride, and the magnetic adsorbent is magnetic Fe3O4 nano particles with the surfaces covered with polydopamine coatings. The magnetic adsorbent provided by the invention has the superparamagetic nanoparticles, the polydopamine coatings on the surfaces of the nano particles are rich in amino groups and hydroxyl groups, under the salt solution environment provided by the nucleic acid binding solution, nucleic acids can be efficiently adsorbed, the nucleic acid adsorbing efficiency and quality of the existing magnetic nano particles are improved, and the nucleic acids can be extracted efficiently and rapidly; and when the nucleic acid extraction kit is applied to extraction of human whole blood, blood treatment is not needed, and the nucleic acid extraction kit is suitable for gene diagnosis and treatment of clinical diseases. The nucleic acid extraction method provided by the invention uses the nucleic acid extraction kit, the quality and efficiency of extracting of the nucleic acids are improved, and the nucleic acid extraction method is suitable for application and promotion of clinical diagnosis and basic scientific research.

The invention provides a preparation method of magnetic micro-beads for extracting nucleic acid and relates to the preparation method of the magnetic micro-beads, aiming at solving the problems of existing biological magnetic beads that the grain diameter is large, the surface energy is small and the adsorption efficiency of the nucleic acid is low. The method 1 comprises the following steps: 1, preparing Fe3O4 / SiO2 magnetic micro-beads; 2, washing the magnetic micro-beads; 3, preparing magnetic micro-beads bonded with epoxy groups on the surfaces; 4, preparing magnetic micro-beads bonded with glycol groups on the surfaces. The method 2 comprises the following steps: 1, preparing the Fe3O4 / SiO2 magnetic micro-beads; 2, washing the magnetic micro-beads; 3, preparing the magnetic micro-beads bonded with the epoxy groups on the surfaces; 4, preparing magnetic micro-beads coated with iminodiacetic acid on the surfaces. The grain diameter of the magnetic beads prepared by the method provided by the invention is small and is nano-grade; the surface energy of the magnetic beads is improved and suspension time of the magnetic beads in an extracting solution is prolonged; the magnetic beads can be in more efficient contact with target extract, and the extracting efficiency of the target extract is improved. The preparation method is used for preparing the magnetic micro-beads for extracting the nucleic acid.

The invention provides a layout characteristic parameter extraction method for graphic data of a grid point edge region. The method comprises the steps of performing grid point division on original design layout data for at least four times, and during the grid point division, expanding all edges of a grid point from inside to outside of a graph to form an annular region; distinguishing a graph in the grid point from an edge graph of the grid point; calculating the weight line width, perimeter and density of the graph in the grid point to obtain calculation results and influence factor localization; calculating the weight line width, perimeter and density of the graph in the grid point edge region to obtain calculation results and influence factor localization; and calculating geometric characteristic parameter values of all grid points.

The invention relates to the technical field of detection of baijiu ingredients, in particular to a method for determining the squalene content of baijiu. According to the method, conditions such as the extraction stage number, the dilution of a liquor sample, types of organic solvents, chromatographic column selection and the like are optimized, and the method combining multistage-LIME (liquid-liquid micro-extraction) and GC-MS (gas chromatography-mass spectrometry) for determining the squalene content of baijiu is established. The method has the advantages of simplicity, convenience, accuracy, sensitivity and rapidness, the limit of detection of the squalene is 0.17 mu g / L, the limit of quantitation is 0.55 mu g / L, the determined recovery rate of the squalene in 11 types of baijiu ranges from 85.12% to 113.64%, the relative standard deviation is smaller than 13.80%, and quantitative analysis of trace triterpene with biological activity and with the content of ng / L or mu g / L level in baijiu can be realized.

The invention relates to a method for extracting flavonoid compounds in mung bean hull, comprising the following specific steps: firstly grinding one part of mung bean hull, then performing extrusionand swelling treatment to the grinded mung bean hull, using organic solution to extract from the grinded puffed material, filtrating to collect extract, performing lyophilization concentration treatment to the extract and obtaining raw flavonoid compounds of mung bean hull. The invention combines the double screw extrusion and swelling technology and organic solvent extraction process to extract flavonoid compounds of mung bean hull, shortens the extraction time, improves the extraction rate, simplifies the operation and is applicable to industrialized production.

The invention provides an extraction method of natural plant polysaccharide and belongs to the field of natural product preparation. The extraction method comprises the steps of inoculating enzyme-producing bacteria capable of secreting cell wall degrading enzyme to an enzyme-producing fermentation culture medium, then adding natural plant powder for incubating and fermenting, mixing an obtained fermentation liquid with an alcohol solution, and conducting precipitation. The extraction method greatly simplifies the extraction steps of the natural plant polysaccharide, reduces the extraction cost, improves the efficiency of extracting the natural plant polysaccharide, avoids the use of toxic and harmful reagents including ether, chloroform and the like, and is beneficial to mass applicationof the natural plant polysaccharide in animal husbandry.

The invention relates to a method for extracting useful substances from shrimp shells. The method comprises the following steps: crushing the shrimp shells, mixing the crushed shrimp shells and water,performing heating to 28-35 DEG C, adjusting the pH to 6.8-7.5, preferably, 6.8-7, adding an alkaline protease, performing mixing, performing heating to 42-48 DEG C, performing constant-temperature enzymolysis for 50-70 min, and performing sieving to obtain an enzymatic hydrolysate and solid residues; performing centrifugal separation treatment on the enzymatic hydrolysate to obtain a shrimp protein deposit containing astaxanthin; mixing the shrimp protein deposit and water, performing heating while stirring, adjusting the pH to 6.8-7.0, performing heating to 58-60 DEG C, adding vegetable oil, and performing emulsification for 50-70 min under stirring to obtain an emulsion; and performing centrifugation on the emulsion, and performing layering to obtain oil containing the astaxanthin at the upper layer, water in the middle layer, and a shrimp protein at the lower layer. The method provided by the invention adopts waste biomass obtained after shrimps are processed as a raw material, and can simultaneously extract four high-valued substances, thereby not only improving the utilization rate of raw materials, but also shortening the production cycle; and no organic solvent is added, so that the method is clean, green and environmentally friendly.

The invention discloses a chicken whole blood simple, convenient and quick DNA extraction method which comprises the following steps of after adding chicken whole blood in an anticoagulant, adding a mixture in lysate for mixing, putting a mixture in a water solution boiler with the temperature being 56-60 DEG C for 20-30min, adding a separating medium in a product, putting a mixture in the water solution boiler with the temperature being 70-80 DEG C for 20-30min for centrifugation, taking a supernate, transferring the supernate into another EP pipe, adding a precipitated solution, performing centrifugation, reserving precipitates, adding a washing solution, and performing uniform mixing and centrifugation; and carefully discarding the supernate, collecting the precipitates, drying the precipitates at the room temperature, and adding a TB buffer liquid to obtain a chicken DNA extracting solution. By adopting the chicken whole blood simple, convenient and quick DNA extraction method provided by the invention, the purity and the integrity of extracted DNA can both reach test requirements, and the concentration and the purity are superior to that of DNA extracted by an existing kit. Bycombining with application on a chicken molecular breeding aspect, proved that the method is an environment-friendly DNA extraction method with low cost and simple operation.

The invention discloses a method for searching a similar watershed based on a spatial dimension division theory. The method comprises the following steps: firstly, dividing a drainage basin into a plurality of orthogonal grid units based on DEM data to obtain an elevation in each grid unit, thereby obtaining an elevation grid Raster_Ele of the drainage basin, and calculating to obtain an elevationfractal dimension value Fra_Ele; secondly, calculating the gradient in each grid unit to obtain a gradient grid Raster_Slope of the drainage basin, and calculating to obtain a gradient fractional dimension value Fra_Slope; next, calculating the slope direction in each grid unit to obtain a slope direction grid Raster_Slope Dir of the drainage basin, and calculating to obtain a slope direction sub-dimension value Fra_Slope Dir; and finally, constructing a multi-dimensional feature space, generalizing a drainage basin into data points, labeling the data points in the multi-dimensional feature space, and classifying the data points, so that the drainage basin divided into one class is regarded as a similar drainage basin.

The invention discloses a metagenome extraction method for specifically reducing or removing a host genomic DNA based on a CRISPR-Cas system composition. The invention aims to provide a technique forreducing a host genomic DNA background in metagenome sequencing and improving effective sequencing data of other microorganisms. The CRSIPR-Cas system in the method comprises a plurality of crRNA or crRNA / tracrRNA derivatives, as well as a biotin-labeled Cas protein or variant protein with endonuclease activity deficiency. The sequence of the crRNA or crRNA / tracrRNA derivatives is complemented with a repetitive sequence Alu element region (target sequence) in the host genomic DNA, so that the binding of the Cas protein or the variant thereof with endonuclease activity deficiency is guided to be bonded on the host genomic DNA, the CRISPR-Cas system and the bonded host DNA are captured by adding magnetic beads coated with streptavidin, and thus the concentration of the host genomic DNA in asample solution is reduced and the abundance of nucleic acids of other non-host genomic DNA is improved.

The invention discloses a method for extracting high-activity immunoglobulin in blood, belonging to the range of food and feed additive and bio-chemical detection reagent preparation technology. Said invention is aimed at overcoming the defect of the existent technique and provides the method adopting low-temp. organic solvent and combining it with ultrafiltration process. Said method includes the following steps: firstly, using low-temp. organic solvent to extract immunoglobulin in the blood, then ultrafiltering and purifying immunoglobulin, finally freze-drying or low-temp. spray-drying so as to obtain the invented product. Its immunoglobulin content can be up to above 65%. Said invention can simplify production steps of low-temp. ethyl alcohol precipitation method, can reduce denaturing rate of immunoglobulin, and can recover albumin and ethyl alcohol and can fully recover and utilize wastes.

The invention relates to a method for measuring residual quantity of thidiazuron and diuron as well as metabolites thereof in cotton, which comprises the following concrete steps: weighing 20.0g of sample; putting the sample into a conical flask with a plug; adding 50mL of acetonitrile and 10mL of distilled water; oscillating and extracting for 60min on an oscillator; adding 6.0-10g of sodium chloride into the filtered solution; oscillating for 2-5min; standing for layering; collecting the organic phase at the upper layer; dehydrating by anhydrous sodium sulfate; carrying out decompression concentration in a water bath at 40 DEG C for nearly drying; blow-drying by nitrogen gas; adding 10-20mL of ethyl acetate; then pre-leaching by adopting a C18 column and 5mL of ethyl acetate; sampling; leaching by 10mL of ethyl acetate; collecting the leacheate; carrying out decompression concentration in the water bath at 40 DEG C for nearly drying; blow-drying by nitrogen gas; metering the volume by 2mL of methanol; filtering by a filter membrane; and measuring by adopting a DAD detector. The measuring method of the invention simplifies the extracting steps, reduces the consumption of solventssuch as toluene dichloride and the like, and reduces the interference of impurities in the sample by using solid phase extraction and purification so that the result is more accurate, sensitive and reliable.

The invention discloses a method of preparing a grosvenor momordica fruit extract through continuous countercurrent extraction. The method includes directly feeding grosvenor momordica fruit into a continuous countercurrent extraction device after the grosvenor momordica fruit is crushed, extracting the grosvenor momordica fruit, and performing filtration to remove impurities, decoloring, adsorption purification, and concentration spray drying to obtain the extract. The content of mogroside V in the extract is not less than 40%. According to the method, only pure water or water-containing ethanol is adopted as a purification solvent in the whole process so that the method is safe and nontoxic. The method is simplified and capable of continuous operation so that a large amount of labor and resources can be saved. Preparing equipment and site requirements are low. The method is suitable for industrial large-scale production.

The invention belongs to the technical field of fungal hypha total DNA extraction, and particularly relates to a fungal hypha total DNA extracting solution and a method for extracting fungal hypha total DNA. The fungal hypha total DNA extracting solution comprises an extracting solution I and an extracting solution II, wherein the extracting solution I comprises a buffer salt, a nonionic surfactant and a neutral salt water solution, and the pH value of the extracting solution I is 7.5-8.5; and the extracting solution II comprises a buffer salt and a cationic surfactant, and the pH value is 7.5-8.5. The fungal hypha total DNA extracting solution simplifies the total DNA extraction steps, is suitable for extracting trace amounts of total DNA, provides an effective experimental means for the scarce experimental materials, avoids the use of the toxic organic solvents, can simplify the operation steps and shorten the time, and avoids the possibility of high tendency to cause frost damage to skin in the liquid nitrogen grinding process.