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Method for extracting Sargassum furiforme polysaccharides

A hijiki polysaccharide and hijiki technology, applied in the biological field, can solve the problems of low polysaccharide content and purity, high energy consumption, complicated operation, etc., and achieve the effect of stable process, simple extraction steps and high purity

Active Publication Date: 2016-05-25
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction techniques of fucoidan polysaccharides mainly include enzymatic hydrolysis, alkaline hydrolysis, acid hydrolysis, water extraction, and ultrasonic-assisted extraction. More energy consumption materials, polysaccharide content and purity are not high

Method used

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  • Method for extracting Sargassum furiforme polysaccharides
  • Method for extracting Sargassum furiforme polysaccharides
  • Method for extracting Sargassum furiforme polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Dry Hijiki at 50°C, pulverize into powder, and pass through a 100-mesh sieve;

[0034] (2) 15g of the hijiki algae powder obtained in step (1) is fully mixed with 95% ethanol, and the reflux degreasing temperature is 70°C, hijiki algae powder: 95% ethanol=1: 10 (m:v ), time 2h / time, repeated 5 times.

[0035] (3) Recover the algae powder after degreasing in step (2), according to algae powder: distilled water=30: 1 (m: v) solid-liquid ratio, add distilled water, after fully stirring and mixing and soaking for 12h, filter with two layers of 200-mesh silk sieves , collect the filtrate for the first time, add distilled water in the above ratio to the filter residue and continue to stir and mix thoroughly, then collect the filtrate every 2h, repeat 3 times. The combined filtrates were concentrated under reduced pressure at 50°C to 200 mL.

[0036] (4) For the concentrated solution in step (3), add 95% ethanol according to concentrated solution: 95% ethanol = 1:4 (v: v...

Embodiment 2

[0040] (1) Dry hijiki at 50°C, pulverize into powder, and pass through a 40-mesh sieve;

[0041] (2) 15g of the hijiki algae powder obtained in step (1) is fully mixed with 95% ethanol, and the reflux degreasing temperature is 70°C, hijiki algae powder: 95% ethanol=1: 10 (m:v ), time 2h / time, repeated 3 times.

[0042] (3) Recover the algae powder after degreasing in the step (2), according to distilled water: algae powder=10:1 (m:v) solid-liquid ratio, add distilled water, fully stir and mix and soak after 12h, filter with two layers of 200 mesh silk sieves , collect the filtrate for the first time, add the same proportion of distilled water to the filter residue and continue to stir and mix thoroughly, then collect the filtrate every 2h, repeat 3 times. The combined filtrates were concentrated under reduced pressure at 50°C to 200 mL.

[0043] (4) For the concentrated solution in step (3), add 95% ethanol according to concentrated solution: 95% ethanol = 1:1 (v:v), and aft...

Embodiment 3

[0047](1) Dry Hijiki at 50°C, pulverize into powder, and pass through a 200-mesh sieve;

[0048] (2) 15g of the hijiki algae powder obtained in step (1) is fully mixed with 95% ethanol, and the reflux degreasing temperature is 70°C, hijiki algae powder: 95% ethanol=1: 10 (m:v ), time 2h / time, repeat once.

[0049] (3) Recover the algae powder after degreasing in step (2), according to distilled water: algae powder=50:1 (m:v) solid-liquid ratio, add distilled water, after fully stirring and mixing and soaking for 12h, filter with two layers of 200-mesh silk sieves , collect the filtrate for the first time, add the same proportion of distilled water to the filter residue and continue to mix thoroughly with the mixer, then collect the filtrate every 2h, repeat 1 time. The combined filtrates were concentrated under reduced pressure at 50°C to 200 mL.

[0050] (4) For the concentrated solution in step (3), add 95% ethanol according to concentrated solution: 95% ethanol = 1:3 (v: ...

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Abstract

The invention provides a method for extracting Sargassum furiforme polysaccharides, which has the advantages of simple operation steps, low energy and material consumption, higher polysaccharide content and higher purity. The method comprises the following steps: (1) drying Sargassum furiforme, pulverizing the Sargassum furiforme into powder, and passing the powder through a 40-200-mesh screen; (2) thoroughly mixing the Sargassum furiforme alga powder obtained in the step (1) with 95% ethanol, and carrying out reflux degreasing; (3) recycling the degreased alga powder in the step (2), adding distilled water, filtering through bolting silk several times, collecting the filtrates, merging the filtrates, and concentrating under reduced pressure to obtain a concentrated solution; (4) adding 95% ethanol into the concentrated solution in the step (3), carrying out alcohol precipitation, centrifugating, collecting the precipitate, adding distilled water, and stirring until the solid is completely dissolved; and (5) carrying out freeze vacuum drying on the polysaccharide solution in the step (4) to obtain the polysaccharide product.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting hijiki polysaccharides. Background technique [0002] Sargassum fusiforme (Harv.) Setch is a plant of the genus Sargassum in Phaeophyta, also known as goat's milk, sea barley, etc. It is widely distributed, and it is distributed along the coasts of Japan and North Korea, and it is more distributed in the coastal areas of Zhejiang. In Japan, hijiki is called "longevity vegetable"; in my country, hijiki has a long history of being used as medicine, and "Shen Nong's Materia Medica" and "Compendium of Materia Medica" record its edible and medicinal value. Hijiki itself is rich in nutrients, of which carbohydrates account for a large proportion, endowing it with more biological functions and higher development value. Hijiki is widely used in dietary therapy such as lowering blood fat, antithrombotic, antitumor, eliminating brain fatigue, enhancing bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04
CPCC08B37/0003C08B37/0063
Inventor 吴明江何丹张旭陈培超李伟赵萍
Owner WENZHOU UNIVERSITY
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